LOCUS CS769-Fina 5326 bp DNA circular SYN 13-JUN-2014 DEFINITION pGEX-4T1. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|700656484| COMMENT VNTDBDATE|700656484| COMMENT LSOWNER| COMMENT VNTNAME|CS769-Final-02-pGEX-4T-1-RNaseA| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers source 1..5326 /organism="pGEX-4T1" /mol_type="other DNA" /vntifkey="98" promoter 184..212 /vntifkey="30" /label=tac_promoter misc_feature 224..246 /vntifkey="21" /label=M13_pUC_rev_primer misc_feature 258..935 /vntifkey="21" /label=GST misc_feature 869..891 /vntifkey="21" /label=pGEX_5_primer misc_feature complement(1376..1398) /vntifkey="21" /label=pGEX_3_primer promoter 1664..1692 /vntifkey="30" /label=AmpR_promoter CDS 1734..2594 /gene="Ampicillin" /vntifkey="4" /label=Ampicillin /note="ORF frame 3" gene 1734..2594 /gene="Ampicillin" /vntifkey="60" rep_origin 2749..3368 /vntifkey="33" /label=pBR322_origin misc_feature 3666..4757 /vntifkey="21" /label=lacI CDS 3798..4757 /vntifkey="4" /label=ORF\frame\3 promoter 4806..4835 /vntifkey="30" /label=lac_promoter misc_feature 4849..4871 /vntifkey="21" /label=M13_pUC_rev_primer misc_feature 4870..4888 /vntifkey="21" /label=M13_reverse_primer misc_feature complement(4900..4916) /vntifkey="21" /label=M13_forward20_primer misc_feature complement(4909..4931) /vntifkey="21" /label=M13_pUC_fwd_primer misc_feature 4897..5052 /vntifkey="21" /label=lacZ_a exon 936..1310 /vntifkey="61" /label=RNaseA BASE COUNT 1325 a 1307 c 1378 g 1316 t ORIGIN 1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccaagga aactgcagca gccaagtttg 961 agcggcagca catggactcc agcacttccg ctgccagcag ctccaactac tgtaaccaga 1021 tgatgaagag ccggaacctg accaaagatc gatgcaagcc agtgaacacc tttgtgcacg 1081 agtccctggc tgatgtccag gccgtgtgct cccagaaaaa tgttgcctgc aagaatgggc 1141 agaccaattg ctaccagagc tactccacca tgagcatcac cgactgccgt gagaccggca 1201 gctccaagta ccccaactgt gcctacaaga ccacccaggc gaataaacac atcattgtgg 1261 cttgtgaggg aaacccgtac gtgccagtcc actttgatgc ttcagtgtag ctcgagcggc 1321 cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 1381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 1441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 1501 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat 1561 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 1621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 1681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 1741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 1801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 1861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 1921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 1981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 2041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 2101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 2161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 2221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 2281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 2341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 2401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 2461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 2521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 2581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 2641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 2701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2881 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 3421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 3481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 3541 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata aattccgaca 3601 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat 3661 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 3721 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 3781 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 3841 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 3901 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 3961 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 4021 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 4081 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 4141 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 4201 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 4261 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 4321 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 4381 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 4441 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 4501 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 4561 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 4621 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 4681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 4741 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 4801 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 4861 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg 4921 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 4981 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 5041 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg 5101 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca 5161 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt 5221 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg 5281 gctacaggaa ggccagacgc gaattatttt tgatggcgtt ggaatt //