LOCUS Exported 8055 bp DNA circular SYN 10-SEP-2025 DEFINITION Exported. ACCESSION V010426 VERSION . KEYWORDS pLVX-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8055) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 8055) TITLE Direct Submission REFERENCE 3 (bases 1 to 8055) TITLE Direct Submission REFERENCE 4 (bases 1 to 8055) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 10, 2025 from SnapGene 6.0.2 https://www.snapgene.com COMMENT SGRef: number: 1; type: "Journal Article" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8055 /mol_type="other DNA" /organism="synthetic DNA construct" source join(6799..8055,1..6798) /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 46..279 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 464..508 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 657..698 /label=Protein Tat /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 770..887 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 944..1247 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1248..1451 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 1545..1610 /label=MCS /note="multiple cloning site" promoter 1633..2132 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 2153..2749 /label=PuroR /note="puromycin N-acetyltransferase" misc_feature 2766..3354 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 3561..4194 /label=5' LTR /note="5' long terminal repeat (LTR) from HIV-1" primer_bind complement(4323..4339) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4347..4363) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4371..4401) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4416..4437) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4725..5313) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5487..6344) /label=AmpR /note="beta-lactamase" promoter complement(6345..6449) /label=AmpR promoter polyA_signal 6497..6631 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" LTR 6799..7432 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 7479..7604 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" ORIGIN 1 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct 61 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca 121 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga 181 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat 241 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg 301 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga 361 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag 421 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt 481 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg 541 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc 601 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca 661 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag 721 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc tttaaaagaa 781 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca 841 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca 901 gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca taacttacgg 961 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1021 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1081 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1141 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1201 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1261 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1321 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 1381 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 1441 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 1501 acctccatag aagacaccga ctctactaga ggatcgctag cgctaccgga ctcagatctc 1561 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc cgcgactcta 1621 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 1681 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 1741 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 1801 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 1861 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 1921 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 1981 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 2041 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 2101 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga 2161 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 2221 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 2281 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 2341 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 2401 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 2461 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 2521 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 2581 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 2641 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 2701 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct 2761 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 2821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 2881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 2941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 3001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 3061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 3121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 3181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 3241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 3301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 3361 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 3421 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 3481 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 3541 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 3601 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 3661 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 3721 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 3781 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 3841 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 3901 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 3961 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 4021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 4081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 4141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 4201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 4261 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca 4321 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 4381 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 4441 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 4501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 4561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 4621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 4681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 4741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 4801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 4861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 4921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 4981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 5041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 5101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 5161 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 5221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 5281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 5341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 5401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 5461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 5521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 5581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 5641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 5701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 5761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 5821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 5881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 5941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 6001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 6061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 6121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 6181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 6241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 6301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 6361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 6421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 6481 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 6541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 6601 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 6661 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 6721 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 6781 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg 6841 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc 6901 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag 6961 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc 7021 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat 7081 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt 7141 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg 7201 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc 7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 7441 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 7501 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 7561 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 7621 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 7681 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 7741 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 7801 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 7861 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 7921 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat 7981 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 8041 agtagcaccc accaa //