LOCUS pET21a_Human\ann 6330 bp DNA circular 4-SEP-2025 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-16133312| COMMENT VNTDBDATE|-16133312| COMMENT LSOWNER| COMMENT VNTNAME|pET21a_Human annexin V| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(LOGPEN 0 6 10053171) 8 1 4.33078 1.9 COMMENT 0.082322 1) COMMENT #145=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #72# 268673068 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (1961)" "@N (@S)" 5 13.7355 COMMENT -5.22699 0 -10 5.34495 0.974079 #144#) COMMENT #146=(CScratch COMMENT (CWidget 3 (1 #0# 3) 1 2 0 0 #80# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.83526 1.9 COMMENT 0.082322 1) COMMENT #147=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #72# 268673104 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (2461)" "@N (@S)" 5 11.202 COMMENT -8.71165 0 -10 5.3949 0.974079 #146#) COMMENT #148=(CScratch COMMENT (CWidget 4 (1 #0# 4) 1 2 0 0 #80# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 1.91763 1.9 COMMENT 0.082322 1) COMMENT #149=(CLabel (CWidget 0 (0 0) 1 2 0 0 #72# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (4396)" "@N (@S)" 5 -14.09 COMMENT -3.48466 0 -10 5.46983 0.974079 #148#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #150=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -393 100) COMMENT (CObjectList) (CObjectList)) COMMENT #151=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #152=(AnlzFontColorItem 5116 5134 37173247) COMMENT #153=(AnlzFontColorItem 6201 6329 37173247)) 1 COMMENT (CObList #154=(AnlzFontBackColorItem 5204 6166 40304639))) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_feature 5117..5135 /vntifkey="21" /label=T7\promoter misc_feature 6202..6330 /vntifkey="21" /label=T7\terminator misc_feature 5205..6167 /vntifkey="21" /label=Human\annexin\V BASE COUNT 1510 a 1634 c 1688 g 1498 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatggca caggtacttc 5221 gcggcaccgt gacggatttt ccgggcttcg atgaacgcgc agatgcggag accctgcgta 5281 aggcgatgaa agggctgggt actgatgagg agtctatcct gacgcttctt accagccgca 5341 gtaacgcgca acgtcaggaa atcagtgcgg cgttcaagac cttgtttggg cgtgatctgc 5401 tggatgatct taaaagcgag ctgacgggaa aattcgaaaa gctgatcgtt gctctgatga 5461 aaccgagtcg tctgtacgat gcgtatgagt taaagcacgc gctgaagggt gctggcacca 5521 atgaaaaagt gcttacggaa attattgcat cacgtacccc ggaagagctg cgtgccatca 5581 aacaagtgta cgaagaagaa tatggctcaa gtctggaaga cgacgtggtc ggtgatacct 5641 ctggttatta tcaacgcatg ttggttgttt tacttcaggc gaaccgcgac ccggacgctg 5701 gtatcgacga ggcgcaggtg gaacaggatg cccaggcctt atttcaagcg ggcgaactga 5761 aatggggcac ggatgaagaa aaatttatta ctattttcgg aacccgctcc gtgagtcacc 5821 ttcgtaaggt gtttgacaag tacatgacga tcagcggttt ccagatcgaa gaaaccatcg 5881 atcgcgaaac gagtggcaac ttagaacaat tgttactggc cgtagtgaaa tcaatccgta 5941 gtattccggc ctatcttgcg gaaacccttt actacgcaat gaaaggagcg ggcacggatg 6001 atcacaccct tatccgtgtg atggttagtc gttcagagat tgacttgttt aacattcgca 6061 aagagttccg caagaacttc gcgacaagtc tgtattccat gatcaaaggt gataccagtg 6121 gcgattacaa aaaagccctg ctgctgctgt gtggcgaaga tgactaactc gagcaccacc 6181 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg 6241 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 6301 ttttgctgaa aggaggaact atatccggat //