LOCUS pcDNA3.1(+)-koza 7359 bp DNA circular SYN 21-OCT-2021 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14068047| COMMENT VNTDBDATE|-14096835| COMMENT LSOWNER| COMMENT VNTNAME|pcDNA3.1(+)-kozak-IDUA-6His| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|Invitrogen| COMMENT VNTOAUTHORTEL|(800)-357-3114| COMMENT VNTOAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTOAUTHORAD1|Invitrogen Corporation| COMMENT VNTOAUTHORAD2|1620 Faraday Avenue| COMMENT VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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-10.454 0 -10 4.69556 0.949103 #226#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #228=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 757089353 100) COMMENT (CObjectList) (CObjectList)) COMMENT #229=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -393 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #230=(AnlzFontColorItem 924 2932 33554432)) 0 COMMENT (CObList #231=(AnlzFontBackColorItem 922 2886 40304639) COMMENT #232=(AnlzFontBackColorItem 2905 2907 40304639) COMMENT #233=(AnlzFontBackColorItem 2887 2904 43633766))) (CObList) 48163828 COMMENT (CStringList) 401 18066864 (CObList))) FEATURES Location/Qualifiers source 1..7359 /invitrogen="124" /vntifkey="98" CDS complement(6363..7223) /invitrogen="1020000" /vntifkey="4" /label=Amp(R) polyA_site 2959..3183 /invitrogen="1870000" /vntifkey="26" /label=BGH\pA primer complement(2953..2970) /invitrogen="1910000" /vntifkey="27" /label=BGH\reverse\primer promoter complement(7224..7322) /invitrogen="2330000" /vntifkey="30" /label=bla\promoter primer 769..789 /invitrogen="1930000" /vntifkey="27" /label=CMV\forward\primer promoter 232..819 /invitrogen="2180000" /vntifkey="29" /label=CMV\promoter rep_origin 3229..3657 /invitrogen="2500000" /vntifkey="33" /label=f1\origin misc_feature 895..2941 /invitrogen="1630000" /vntifkey="21" /label=MCS CDS 4067..4861 /invitrogen="1260000" /vntifkey="4" /label=Neo(R) rep_origin complement(5548..6218) /invitrogen="2540000" /vntifkey="33" /label=pUC\origin promoter 3662..4031 /invitrogen="2290000" /vntifkey="29" /label=SV40\early\promoter polyA_signal 5035..5165 /invitrogen="1840000" /vntifkey="25" /label=SV40\pA primer 863..882 /invitrogen="2100000" /vntifkey="27" /label=T7\primer promoter 863..879 /invitrogen="2370000" /vntifkey="30" /label=T7\promoter misc_feature 923..928 /vntifkey="21" /label=kozak misc_feature 929..2887 /vntifkey="21" /label=IDUA misc_feature 2888..2905 /vntifkey="21" /label=6His BASE COUNT 1666 a 1988 c 1853 g 1852 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgccaccat gcgtcccctt cgaccacgag cagccctcct 961 tgcactgctt gccagtctgt tggctgcccc ccctgttgcc cctgccgagg ccccacattt 1021 ggttcaagtc gacgcagcac gggcactctg gcctcttcgt cggttttggc ggtctacagg 1081 gttttgccca ccccttcccc actcccaagc tgatcagtat gttctgtcct gggatcagca 1141 actgaatctg gcatatgttg gtgccgttcc ccaccggggc atcaagcaag taagaacaca 1201 ttggttgctg gagttggtca ccacccgtgg gtcaaccggt cgagggcttt catataactt 1261 cacacacttg gacggctatc ttgacttgct cagagaaaac caactgctcc ctggcttcga 1321 gctcatgggc tcagcctctg gccatttcac cgattttgaa gacaagcaac aggtattcga 1381 atggaaagac ttggtctcct cactggctag aaggtatata ggccgttatg gtttggccca 1441 tgtttccaaa tggaatttcg aaacctggaa tgagcctgac caccatgact tcgataatgt 1501 atccatgacc atgcaaggtt tcttgaacta ctatgacgca tgctccgagg gtcttcgagc 1561 cgctagccca gcactgcgac ttgggggccc tggggactct ttccatactc ccccacgatc 1621 acccctgtca tggggtcttc ttcgtcattg tcatgacggt acaaactttt tcaccggcga 1681 agccggggtc cgattggact acattagctt gcatcggaag ggtgcaagaa gcagcatctc 1741 catacttgaa caagagaagg tcgttgctca acaaatacga caattgtttc caaagtttgc 1801 tgatacccct atttataacg acgaagcaga ccccttggtg ggttggtccc ttccacaacc 1861 ctggcgcgcc gacgtgacct acgccgctat ggtcgtcaaa gttattgcac agcaccaaaa 1921 tctgttgctg gctaatacta cttcagcatt cccatacgct ttgctctcta acgacaacgc 1981 ttttctgagc taccatccac atcctttcgc tcagcgaacc ctgaccgctc gctttcaagt 2041 gaacaacaca cggcctcctc atgtgcagct cctccggaaa cccgttctga ccgctatggg 2101 cctgctcgca ctcctggatg aagagcagct ttgggctgaa gtgtcccaag ctggaactgt 2161 ccttgattct aatcatactg taggtgtcct ggcttcagcc caccggcccc aaggcccagc 2221 tgacgcttgg agggcagcag ttttgatata cgctagcgat gacaccaggg cccaccctaa 2281 ccggtctgtg gccgttactt tgcggctgag aggtgttcct cctggtcctg gtcttgtata 2341 cgtcacccgg tacttggata acggtctgtg ttcacccgat ggtgaatgga gaaggttggg 2401 cagacctgtt tttcccactg ccgagcagtt tcgcagaatg agagcagcag aagatcctgt 2461 agccgctgca cccagacctc ttccagcagg gggcaggctg acacttcgac cagctctgcg 2521 attgcccagt ctcctcttgg tacacgtatg tgctaggcca gaaaaaccac caggacaggt 2581 taccagactc cgcgctctgc cccttacaca gggccaactt gtactggtgt ggtccgatga 2641 acatgtcggt tcaaagtgtt tgtggactta cgagattcag ttcagtcagg atggaaaagc 2701 ctatactcca gtaagtcgta aaccaagtac ttttaacctc tttgtattta gccctgacac 2761 tggggcagtg agcggtagtt acagggtgcg agctttggac tattgggcac gaccaggccc 2821 tttcagtgat ccagtgccat atctcgaagt gcccgtccca agagggccac catcccccgg 2881 caatccccac caccaccacc accactgagc ggccgctcga gtctagaggg cccgtttaaa 2941 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 3001 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 3061 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 3121 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 3181 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 3241 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 3301 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 3361 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 3421 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 3481 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 3541 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 3601 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 3661 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 3721 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 3781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 3841 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 3901 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 3961 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 4021 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 4081 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4141 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4201 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 4261 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4321 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4381 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4441 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4501 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4561 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 4621 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4681 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4741 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4801 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4861 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 4921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 4981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 5041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 5101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 5161 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 5341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 5401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 5461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 5521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 5581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 5641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 5941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 6001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 6061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 6121 aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa 6181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 6301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 6361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 6421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 7021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 7081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 7141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 7201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 7261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 7321 gttccgcgca catttccccg aaaagtgcca cctgacgtc //