LOCUS pEThis-SMT-ppk 7757 bp DNA circular 25-JUL-2022 SOURCE ORGANISM COMMENT http://www.informaxinc.com http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14288436| COMMENT VNTDBDATE|-14495826| COMMENT LSOWNER| COMMENT VNTNAME|pEThis-SMT-ppk| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CStringList) 46314232 799356382 (CObList))) FEATURES Location/Qualifiers CDS 599..1456 /vntifkey="4" /label=Ap CDS complement(3651..4607) /vntifkey="4" /label=lacI misc_feature 5125..5141 /vntifkey="21" /label=T7P misc_feature 7686..7732 /vntifkey="21" /label=T7T CDS 5249..5541 /vntifkey="4" /label=SMT3 sig_peptide 5228..5245 /vntifkey="94" /label=6*His misc_feature 5350..5350 /vntifkey="21" /label=Bgl-SDM exon 5098..5105 /vntifkey="61" /label=bgl-blunt BASE COUNT 1888 a 1993 c 1997 g 1879 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata 5161 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgagagg 5221 atcgcatcac catcaccatc accatggaat gtcggactca gaagtcaatc aagaagctaa 5281 gccagaggtc aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg 5341 atcttcagaa atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc 5401 gttcgctaaa agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag 5461 aattcaagct gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc 5521 tcacagagaa cagattggtg gggtaatggg tcaggaaaag ctatacatcg aaaaagagct 5581 cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct 5641 gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt tctataaagt 5701 ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct ccaactctca 5761 ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc aggaattcga 5821 cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc tgattaatga 5881 acgccagctc tccgtcaatc aacaaaactg gctgcgtcat tattttaagc agtatctgcg 5941 tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt tcctgaaaga 6001 tgattacacc tatctggcgg tggaaattat ccgtggcgat accatccgtt acgcgctgct 6061 ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg 6121 acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg atattttcaa 6181 aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc gcgatgccga 6241 atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt ccagtctcaa 6301 gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct 6361 ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg tccccggcgg 6421 tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag ccaatctggt 6481 gaacaaacca ctgccgcgtt tacgccatat ttggtttgat aaagcccagt tccgcaatgg 6541 ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca cctttgagca 6601 tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga ttaaaattaa 6661 catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg ccgcacataa 6721 cggtaagaaa gtcaccgtgg tggttgagtt acaggcgcgt ttcgacgaag aagccaacat 6781 tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg cgccggggct 6841 gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag tggtgcgtta 6901 tgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata ctgactattc 6961 gttgctgacc gccgatgcgc gcatcaccaa cgaagtacgg cgggtattta actttattga 7021 aaacccatac cgtccggtga catttgatta tttaatggta tcgccgcaaa actcccgccg 7081 cctattgtat gaaatggtgg accgcgagat cgccaacgcg cagcaagggc tgcccagtgg 7141 tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc tgtatgcggc 7201 ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc tgatccccaa 7261 tctggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt accttgaaca 7321 tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt cttccgccga 7381 ctggatgacg cgcaatattg attatcgtat tgaagtggcg acgccgctgc tcgatccgcg 7441 cctgaagcag cgggtactgg acatcatcga catattgttc agcgatacgg tcaaagcacg 7501 ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc gcaaagtacg 7561 ggcgcagttg gcgatttatg actacatcaa atcactcgaa caacctgaat aactcgagtg 7621 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca 7681 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg 7741 aggaactata tccggat //