LOCUS pET21a-BAP 6783 bp DNA circular 17-JAN-2022 DEFINITION Complementary copy of pET-21a. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14213889| COMMENT VNTDBDATE|-14223925| COMMENT LSOWNER| COMMENT VNTNAME|pET21a-BAP| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pET21a-BAP" 0 6783 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 1 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "EcoRI" "HindIII" "NcoI" "NdeI" COMMENT "PstI" "SmaI" "XhoI" "XmaI") (CStringList "atg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 1 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6783 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 715 0 1961 0 2461 0 4396 COMMENT 0 0 "") #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 6622 0 0 "") COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 6000 0 6331 0 0 "") COMMENT #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 6096 0 0 "") COMMENT #6=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1145 0 0 "") COMMENT #7=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #8=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") COMMENT #9=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 5204 0 0 "") COMMENT #10=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6622 0 0 "") COMMENT #11=(CRSite (CStringList) "atg" "atg" 1 0 115 7 0 234 0 408 0 489 0 536 COMMENT 0 548 0 569 0 599 0 696 0 794 0 797 0 820 0 891 0 936 0 941 0 958 0 COMMENT 977 0 1055 0 1065 0 1101 0 1136 0 1148 0 1223 0 1347 0 1400 0 1404 COMMENT 0 1556 0 2023 0 2188 0 2212 0 2276 0 2408 0 2457 0 2486 0 2538 0 COMMENT 2643 0 2730 0 2782 0 2808 0 2842 0 2871 0 2880 0 2889 0 2904 0 2926 COMMENT 0 2929 0 2935 0 2978 0 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COMMENT 0 6659 0 6708 0 6717 0 6734 0 0 "") COMMENT #14=(CRSite (CStringList) "tta" "tta" 1 0 84 32 0 54 0 176 0 189 0 197 0 COMMENT 226 0 303 0 368 0 401 0 418 0 429 0 441 0 452 0 459 0 512 0 627 0 COMMENT 727 0 807 0 833 0 930 0 956 0 1001 0 1172 0 1188 0 1211 0 1281 0 COMMENT 1371 0 1446 0 1474 0 1489 0 1499 0 1513 0 1518 0 1570 0 1796 0 1856 COMMENT 0 1893 0 1919 0 2137 0 2240 0 2291 0 2319 0 2424 0 2497 0 2609 0 COMMENT 2779 0 2811 0 2920 0 2943 0 3031 0 3131 0 3628 0 3633 0 3692 0 3872 COMMENT 0 4123 0 4163 0 4306 0 4357 0 4408 0 4480 0 4718 0 4829 0 4845 0 COMMENT 5116 0 5180 0 5186 0 5235 0 5241 0 5248 0 5317 0 5343 0 5380 0 5404 COMMENT 0 5443 0 5459 0 5485 0 5502 0 5509 0 5842 0 5857 0 5875 0 5969 0 COMMENT 6473 0 0 "") COMMENT #15=(CRSite (CStringList) "tga" "tga" 1 0 137 66 0 223 0 232 0 260 0 282 COMMENT 0 364 0 409 0 414 0 549 0 563 0 584 0 600 0 684 0 700 0 763 0 795 0 COMMENT 798 0 844 0 892 0 901 0 942 0 978 0 982 0 1008 0 1078 0 1098 0 1102 COMMENT 0 1126 0 1288 0 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"2632..2654") COMMENT #23=(CFSignal (CObList) "ROP" 21 0 1 2648 2839 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2648..2839)") COMMENT #24=(CFSignal (CObList) "ORF frame 3" 4 0 1 3648 4607 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3648..4607)") COMMENT #25=(CFSignal (CObList) "lacI" 21 0 1 3648 4739 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3648..4739)") COMMENT #26=(CFSignal (CObList) "ORF frame 2" 4 0 1 4480 4980 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4480..4980)") COMMENT #27=(CFSignal (CObList) "pBRrevBam_primer" 21 0 0 5014 5033 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5014..5033") COMMENT #28=(CFSignal (CObList) "T7_promoter" 30 0 0 5117 5135 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5117..5135") COMMENT #29=(CFSignal (CObList) "lacO" 21 0 0 5135 5162 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5135..5162") COMMENT #30=(CFSignal (CObList) "T7_transl_en_RBS" 21 0 0 5180 5196 0 COMMENT (CStringList) 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0 (0 0) 1 2 3 0 180) COMMENT " /organism=\"pET-21 a (+)\"" 1) COMMENT #175=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /mol_type=\"other DNA\"" 1))) COMMENT "Source_1"))) "Source (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #176=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #177=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174640 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #178=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #179=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 715 " 1) COMMENT #180=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 1961 " 1) COMMENT #181=(CLinePar COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191) COMMENT " N3: 2461 " 1) COMMENT #182=(CLinePar COMMENT (CParagraph 0 (1 #0# 4) 1 2 2 0 191) COMMENT " N4: 4396 " 1))))) "ApaLI: 4 sites") COMMENT 1 5 "GTGCAC" "CACGTG") COMMENT #183=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174384 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #184=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #185=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 6622 " 1))))) "AvaI: 1 site") COMMENT 1 5 "CYCGRG" "GRGCYC") COMMENT #186=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174416 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #187=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97175056 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #188=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #189=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 6000 " 1) COMMENT #190=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 6331 " 1))))) "EcoRI: 2 sites") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #191=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174736 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" COMMENT "TTCGAA") COMMENT #192=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174480 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #193=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #194=(CLinePar COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191) COMMENT " N1: 6096 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #195=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174928 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #196=(CGroupPar COMMENT (CParagraph 0 (10 #9# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #197=(CLinePar COMMENT (CParagraph 0 (1 #9# 1) 1 2 2 0 191) COMMENT " N1: 5204 " 1))))) "NdeI: 1 site") COMMENT 2 4 "CATATG" "GTATAC") COMMENT #198=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174768 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #199=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #200=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 1145 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #201=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97175088 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" COMMENT "GGGCCC") COMMENT #202=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97186000 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #203=(CGroupPar COMMENT (CParagraph 0 (10 #10# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #204=(CLinePar COMMENT (CParagraph 0 (1 #10# 1) 1 2 2 0 191) COMMENT " N1: 6622 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC") COMMENT #205=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97174832 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" COMMENT "GGGCCC"))) "Restriction/Methylation Map") COMMENT #206=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #207=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #208=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #209=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42882872 (4 #52# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #210=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #211=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4906 - 5142" COMMENT 1))) "4906(d1) (79 codons)") COMMENT #212=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996920 (4 #55# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #213=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #214=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6208 - 6363" COMMENT 1))) "6208(d1) (52 codons)"))) COMMENT "Phase #1 (2 frames)") COMMENT #215=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #216=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41989976 (4 #36# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #217=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #218=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 599 - 1456" COMMENT 1))) "599(d2) (286 codons)") COMMENT #219=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42709464 (4 #42# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #220=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #221=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3614 - 3862" COMMENT 1))) "3614(d2) (83 codons)") COMMENT #222=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42113632 (4 #44# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #223=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #224=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4769 - 5029" COMMENT 1))) "4769(d2) (87 codons)"))) COMMENT "Phase #2 (3 frames)") COMMENT #225=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #226=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41991712 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #227=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #228=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2871 - 3236" COMMENT 1))) "2871(d3) (122 codons)") COMMENT #229=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42708680 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #230=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #231=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3462 - 3611" COMMENT 1))) "3462(d3) (50 codons)") COMMENT #232=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996416 (4 #54# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #233=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #234=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5205 - 6617" COMMENT 1))) "5205(d3) (471 codons)"))) COMMENT "Phase #3 (3 frames)"))) "Direct strand") COMMENT #235=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #236=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 -1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #237=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997312 (4 #45# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #238=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #239=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 889 - 1056" COMMENT 1))) "889(c1) (56 codons)") COMMENT #240=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997088 (4 #46# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #241=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #242=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1066 - 1329" COMMENT 1))) "1066(c1) (88 codons)") COMMENT #243=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997760 (4 #47# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #244=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #245=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2275 - 2454" COMMENT 1))) "2275(c1) (60 codons)") COMMENT #246=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997648 (4 #48# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #247=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #248=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2539 - 2745" COMMENT 1))) "2539(c1) (69 codons)") COMMENT #249=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996472 (4 #49# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #250=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #251=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3034 - 3231" COMMENT 1))) "3034(c1) (66 codons)") COMMENT #252=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997592 (4 #50# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #253=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #254=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3631 - 3891" COMMENT 1))) "3631(c1) (87 codons)") COMMENT #255=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997816 (4 #51# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #256=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #257=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4483 - 4980" COMMENT 1))) "4483(c1) (166 codons)"))) COMMENT "Phase #1 (7 frames)") COMMENT #258=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 -2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #259=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997200 (4 #41# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #260=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #261=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2946 - 3161" COMMENT 1))) "2946(c2) (72 codons)") COMMENT #262=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997424 (4 #43# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #263=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #264=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3651 - 4607" COMMENT 1))) "3651(c2) (319 codons)") COMMENT #265=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996248 (4 #57# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #266=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #267=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4980 - 5204" COMMENT 1))) "4980(c2) (75 codons)") COMMENT #268=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996080 (4 #58# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #269=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #270=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5601 - 5756" COMMENT 1))) "5601(c2) (52 codons)"))) COMMENT "Phase #2 (4 frames)") COMMENT #271=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 -3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #272=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997480 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #273=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #274=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2651 - 2872" COMMENT 1))) "2651(c3) (74 codons)") COMMENT #275=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996304 (4 #39# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #276=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #277=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3239 - 3592" COMMENT 1))) "3239(c3) (118 codons)") COMMENT #278=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41997704 (4 #53# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #279=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #280=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4178 - 4345" COMMENT 1))) "4178(c3) (56 codons)") COMMENT #281=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41996864 (4 #56# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #282=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #283=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6023 - 6190" COMMENT 1))) "6023(c3) (56 codons)"))) COMMENT "Phase #3 (4 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misc_feature complement(2648..2839) /vntifkey="21" /label=ROP gene complement(3314..3589) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3648..4607) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(3648..4739) /vntifkey="21" /label=lacI CDS complement(4480..4980) /vntifkey="4" /label=ORF\frame\2 misc_feature 5014..5033 /vntifkey="21" /label=pBRrevBam_primer gene complement(4822..5085) /gene="tet (300 - 563)" /vntifkey="60" promoter 5117..5135 /vntifkey="30" /label=T7_promoter misc_feature 5135..5162 /vntifkey="21" /label=lacO misc_feature 5180..5196 /vntifkey="21" /label=T7_transl_en_RBS misc_feature 5204..5207 /vntifkey="21" /label=Xpress_fwd_primer misc_feature 5205..5207 /vntifkey="21" /label=T7_leader misc_feature complement(6697..6715) /vntifkey="21" /label=T7_Terminal_primer terminator 6655..6783 /vntifkey="43" /label=T7_terminator source complement(1..6783) /organism="pET-21 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 5205..6620 /vntifkey="21" /label=BAP BASE COUNT 1627 a 1790 c 1807 g 1559 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa caaagcacta 5221 ttgcactggc actcttaccg ttactgttta cccctgtgac aaaagcccgg acaccagaaa 5281 tgcctgttct ggaaaaccgg gctgctcagg gcgatattac tgcacccggc ggtgctcgcc 5341 gtttaacggg tgatcagact gccgctctgc gtgattctct tagcgataaa cctgcaaaaa 5401 atattatttt gctgattggc gatgggatgg gggactcgga aattactgcc gcacgtaatt 5461 atgccgaagg tgcgggcggc ttttttaaag gtatagatgc cttaccgctt accgggcaat 5521 acactcacta tgcgctgaat aaaaaaaccg gcaaaccgga ctacgtcacc gactcggctg 5581 catcagcaac cgcctggtca accggtgtca aaacctataa cggcgcgctg ggcgtcgata 5641 ttcacgaaaa agatcaccca acgattctgg aaatggcaaa agccgcaggt ctggcgaccg 5701 gtaacgtttc taccgcagag ttgcaggatg ccacgcccgc tgcgctggtg gcacatgtga 5761 cctcgcgcaa atgctacggt ccgagcgcga ccagtgaaaa atgtccgggt aacgctctgg 5821 aaaaaggcgg aaaaggatcg attaccgaac agctgcttaa cgctcgtgcc gacgttacgc 5881 ttggcggcgg cgcaaaaacc tttgctgaaa cggcaaccgc tggtgaatgg cagggaaaaa 5941 cgctgcgtga acaggcacag gcgcgtggtt atcagttggt gagcgatgct gcctcactga 6001 attcggtgac ggaagcgaat cagcaaaaac ccctgcttgg cctgtttgct gacggcaata 6061 tgccagtgcg ctggctagga ccgaaagcaa cgtaccatgg caatatcgat aagcccgcag 6121 tcacctgtac gccaaatccg caacgtaatg acagtgtacc aaccctggcg cagatgaccg 6181 acaaagccat tgaattgttg agtaaaaatg agaaaggctt tttcctgcaa gttgaaggtg 6241 cgtcaatcga taaacaggat catgctgcga atccttgtgg gcaaattggc gagacggtcg 6301 atctcgatga agccgtacaa cgggcgctgg aattcgctaa aaaggagggt aacacgctgg 6361 tcatagtcac cgctgatcac gcccacgcca gccagattgt tgcgccggat accaaagctc 6421 cgggcctcac ccaggcgcta aataccaaag atggcgcagt gatggtgatg agttacggga 6481 actccgaaga ggattcacaa gaacataccg gcagtcagtt gcgtattgcg gcgtatggcc 6541 cgcatgccgc caatgttgtt ggactgaccg accagaccga tctcttctac accatgaaag 6601 ccgctctggg gctgaaataa ctcgagcacc accaccacca ccactgagat ccggctgcta 6661 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac 6721 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg 6781 gat //