LOCUS pET-His6-BST-BAP 6827 bp DNA circular 10-JAN-2022 SOURCE ORGANISM COMMENT http://www.informaxinc.com http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14213904| COMMENT VNTDBDATE|-14213904| COMMENT LSOWNER| COMMENT VNTNAME|pET-His6-BST-BAP(C-His6)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|JYLIM| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5197..5200" 1))) "TATA"))) COMMENT "TATA Signal (1 total)"))) "Feature Map") COMMENT #118=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #119=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100352832 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #120=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 714 " 1) COMMENT #122=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 1960 " 1) COMMENT #123=(CLinePar COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191) COMMENT " N3: 2460 " 1) COMMENT #124=(CLinePar COMMENT (CParagraph 0 (1 #0# 4) 1 2 2 0 191) COMMENT " N4: 4395 " 1))))) "ApaLI: 4 sites") COMMENT 1 5 "GTGCAC" "CACGTG") COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353152 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "AvaI: 0 sites") 1 5 "CYCGRG" COMMENT "GRGCYC") COMMENT #126=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353184 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #127=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353440 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #128=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #129=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 5891 " 1) COMMENT #130=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 6159 " 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (1 #3# 3) 1 2 2 0 191) COMMENT " N3: 6300 " 1))))) "ClaI: 3 sites") COMMENT 2 4 "ATCGAT" "TAGCTA") COMMENT #132=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353728 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #133=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #134=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 6052 " 1) COMMENT #135=(CLinePar COMMENT (CParagraph 0 (1 #4# 2) 1 2 2 0 191) COMMENT " N2: 6383 " 1))))) "EcoRI: 2 sites") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #136=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353568 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" COMMENT "TTCGAA") COMMENT #137=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100352768 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #138=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #139=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 6148 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #140=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100353024 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #141=(CGroupPar COMMENT (CParagraph 0 (10 #10# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #142=(CLinePar COMMENT (CParagraph 0 (1 #10# 1) 1 2 2 0 191) COMMENT " N1: 5203 " 1))))) "NdeI: 1 site") COMMENT 2 4 "CATATG" "GTATAC") COMMENT #143=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100352864 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #144=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #145=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 1144 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #146=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100352960 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" COMMENT "GGGCCC") COMMENT #147=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100364032 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" COMMENT "GAGCTC") COMMENT #148=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 100352992 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" COMMENT "GGGCCC"))) "Restriction/Methylation Map") COMMENT #149=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #150=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #151=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #152=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244016 (4 #31# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #153=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #154=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 598 - 1455" COMMENT 1))) "598(d1) (286 codons)") COMMENT #155=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244912 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #156=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #157=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3613 - 3861" COMMENT 1))) "3613(d1) (83 codons)") COMMENT #158=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245024 (4 #43# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #159=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #160=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4768 - 5028" COMMENT 1))) "4768(d1) (87 codons)") COMMENT #161=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245416 (4 #48# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #162=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #163=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5257 - 6687" COMMENT 1))) "5257(d1) (477 codons)"))) COMMENT "Phase #1 (4 frames)") COMMENT #164=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #165=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244184 (4 #32# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #166=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #167=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2870 - 3235" COMMENT 1))) "2870(d2) (122 codons)") COMMENT #168=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245136 (4 #34# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #169=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #170=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3461 - 3610" COMMENT 1))) "3461(d2) (50 codons)") COMMENT #171=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244800 (4 #51# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #172=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #173=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6260 - 6415" COMMENT 1))) "6260(d2) (52 codons)"))) COMMENT "Phase #2 (3 frames)") COMMENT #174=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #175=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244464 (4 #47# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #176=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #177=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4905 - 5141" COMMENT 1))) "4905(d3) (79 codons)"))) COMMENT "Phase #3 (1 frame)"))) "Direct strand") COMMENT #178=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #179=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 -1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #180=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245080 (4 #35# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #181=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #182=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 888 - 1055" COMMENT 1))) "888(c1) (56 codons)") COMMENT #183=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244688 (4 #36# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #184=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #185=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1065 - 1328" COMMENT 1))) "1065(c1) (88 codons)") COMMENT #186=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244632 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #187=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #188=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2274 - 2453" COMMENT 1))) "2274(c1) (60 codons)") COMMENT #189=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47243904 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #190=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #191=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2538 - 2744" COMMENT 1))) "2538(c1) (69 codons)") COMMENT #192=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244520 (4 #39# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #193=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #194=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3033 - 3230" COMMENT 1))) "3033(c1) (66 codons)") COMMENT #195=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245192 (4 #41# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #196=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #197=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3630 - 3890" COMMENT 1))) "3630(c1) (87 codons)") COMMENT #198=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244240 (4 #42# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #199=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #200=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4482 - 4979" COMMENT 1))) "4482(c1) (166 codons)") COMMENT #201=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244128 (4 #52# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #202=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #203=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6075 - 6242" COMMENT 1))) "6075(c1) (56 codons)"))) COMMENT "Phase #1 (8 frames)") COMMENT #204=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 -2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #205=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244072 (4 #33# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #206=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #207=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2945 - 3160" COMMENT 1))) "2945(c2) (72 codons)") COMMENT #208=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47243792 (4 #49# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #209=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #210=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3650 - 4606" COMMENT 1))) "3650(c2) (319 codons)") COMMENT #211=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244296 (4 #50# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #212=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #213=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4979 - 5230" COMMENT 1))) "4979(c2) (84 codons)"))) COMMENT "Phase #2 (3 frames)") COMMENT #214=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 -3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #215=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47244576 (4 #44# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #216=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #217=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2650 - 2871" COMMENT 1))) "2650(c3) (74 codons)") COMMENT #218=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47243848 (4 #45# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #219=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #220=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3238 - 3591" COMMENT 1))) "3238(c3) (118 codons)") COMMENT #221=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47243736 (4 #46# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #222=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #223=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4177 - 4344" COMMENT 1))) "4177(c3) (56 codons)") COMMENT #224=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47245472 (4 #53# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #225=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #226=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5653 - 5808" COMMENT 1))) "5653(c3) (52 codons)"))) COMMENT "Phase #3 (4 frames)"))) COMMENT "Complementary strand"))) "Open Reading Frames")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #227=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #228=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #229=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #230=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #231=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #232=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #233=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #234=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #235=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #236=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #237=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #238=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #239=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #240=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #241=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #242=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #243=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #244=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #245=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #246=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #247=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #248=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #249=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #250=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #251=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #252=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #253=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #254=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #255=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #256=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #257=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #258=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #259=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #260=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #261=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #262=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #263=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #264=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #265=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 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#364=(AnlzFontBackColorItem 5215 5232 43633766) COMMENT #365=(AnlzFontBackColorItem 5250 5255 42572451) COMMENT #366=(AnlzFontBackColorItem 5259 6668 40304639) COMMENT #367=(AnlzFontBackColorItem 5256 5258 40304639) COMMENT #368=(AnlzFontBackColorItem 6669 6686 43633766))) (CObList) 1277193313 COMMENT (CStringList) 0 1852270931 (CObList))) FEATURES Location/Qualifiers CDS 598..1455 /vntifkey="4" /label=Amp CDS complement(3650..4738) /vntifkey="4" /label=lacI misc_feature 11..466 /vntifkey="21" /label=f1\origin misc_feature 5216..5233 /vntifkey="21" /label=N-6*His misc_feature 5116..5132 /vntifkey="21" /label=T7\promoter misc_feature 5135..5159 /vntifkey="21" /label=lac\operator misc_feature 6755..6801 /vntifkey="21" /label=T7\terminal RBS 5190..5195 /vntifkey="32" /label=rbs TATA_signal 5197..5200 /vntifkey="41" /label=TATA misc_feature 6670..6687 /vntifkey="21" /label=C-6*His misc_feature 5257..6669 /vntifkey="21" /label=BAP BASE COUNT 1638 a 1805 c 1818 g 1566 t ORIGIN 1 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 61 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 421 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt 481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt 2461 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 2521 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 2581 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 2641 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 2701 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 2761 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 2821 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 2881 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 2941 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 3001 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 3061 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 3121 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga 3181 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 3241 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac 3301 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg 3361 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc 3421 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg 3481 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat 3541 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga 3601 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt 3661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3721 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct 3781 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc 3841 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt 3901 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa 3961 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga 4021 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt 4081 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac 4141 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca 4201 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt 4261 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt 4321 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt 4381 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg 4441 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg 4501 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg 4561 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt 4621 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat 4681 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc 4741 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga 4801 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt 4861 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg 4921 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg 4981 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca 5041 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc 5101 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc 5161 ctctagaaat aattttgttt aactttaaga aggagatata catatgagag gatcgcatca 5221 ccatcaccat cacggatctc tggtgccacg cggatcatga aacaaagcac tattgcactg 5281 gcactcttac cgttactgtt tacccctgtg acaaaagccc ggacaccaga aatgcctgtt 5341 ctggaaaacc gggctgctca gggcgatatt actgcacccg gcggtgctcg ccgtttaacg 5401 ggtgatcaga ctgccgctct gcgtgattct cttagcgata aacctgcaaa aaatattatt 5461 ttgctgattg gcgatgggat gggggactcg gaaattactg ccgcacgtaa ttatgccgaa 5521 ggtgcgggcg gcttttttaa aggtatagat gccttaccgc ttaccgggca atacactcac 5581 tatgcgctga ataaaaaaac cggcaaaccg gactacgtca ccgactcggc tgcatcagca 5641 accgcctggt caaccggtgt caaaacctat aacggcgcgc tgggcgtcga tattcacgaa 5701 aaagatcacc caacgattct ggaaatggca aaagccgcag gtctggcgac cggtaacgtt 5761 tctaccgcag agttgcagga tgccacgccc gctgcgctgg tggcacatgt gacctcgcgc 5821 aaatgctacg gtccgagcgc gaccagtgaa aaatgtccgg gtaacgctct ggaaaaaggc 5881 ggaaaaggat cgattaccga acagctgctt aacgctcgtg ccgacgttac gcttggcggc 5941 ggcgcaaaaa cctttgctga aacggcaacc gctggtgaat ggcagggaaa aacgctgcgt 6001 gaacaggcac aggcgcgtgg ttatcagttg gtgagcgatg ctgcctcact gaattcggtg 6061 acggaagcga atcagcaaaa acccctgctt ggcctgtttg ctgacggcaa tatgccagtg 6121 cgctggctag gaccgaaagc aacgtaccat ggcaatatcg ataagcccgc agtcacctgt 6181 acgccaaatc cgcaacgtaa tgacagtgta ccaaccctgg cgcagatgac cgacaaagcc 6241 attgaattgt tgagtaaaaa tgagaaaggc tttttcctgc aagttgaagg tgcgtcaatc 6301 gataaacagg atcatgctgc gaatccttgt gggcaaattg gcgagacggt cgatctcgat 6361 gaagccgtac aacgggcgct ggaattcgct aaaaaggagg gtaacacgct ggtcatagtc 6421 accgctgatc acgcccacgc cagccagatt gttgcgccgg ataccaaagc tccgggcctc 6481 acccaggcgc taaataccaa agatggcgca gtgatggtga tgagttacgg gaactccgaa 6541 gaggattcac aagaacatac cggcagtcag ttgcgtattg cggcgtatgg cccgcatgcc 6601 gccaatgttg ttggactgac cgaccagacc gatctcttct acaccatgaa agccgctctg 6661 gggctgaaac accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa 6721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa 6781 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggata //