LOCUS N_pET28-N-BAP 6649 bp DNA circular 14-JUN-2022 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14437071| COMMENT VNTDBDATE|-14437071| COMMENT LSOWNER| COMMENT VNTNAME|N_pET28-N-BAP| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "N_pET28-N-BAP" 0 6649 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 1 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "AvaI" "BamHI" "EcoRI" "HindIII" "NcoI" "NdeI" "NheI" COMMENT "NotI" "SacI" "SalI" "SmaI" "XhoI" "XmaI") (CStringList "atg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 1 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6649 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 1068 0 6488 0 0 "") COMMENT #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 5866 0 6197 0 0 "") COMMENT #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5962 0 0 "") COMMENT #5=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") COMMENT #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") COMMENT #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") COMMENT #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") COMMENT #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1070 0 0 "") COMMENT #11=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6488 0 0 "") COMMENT #12=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1068 0 0 "") COMMENT #13=(CRSite (CStringList) "atg" "atg" 1 0 110 7 0 234 0 408 0 489 0 536 COMMENT 0 548 0 584 0 649 0 684 0 788 0 814 0 827 0 980 0 1057 0 1093 0 COMMENT 1118 0 1125 0 1208 0 1320 0 1367 0 1398 0 1423 0 1890 0 2055 0 2079 COMMENT 0 2143 0 2275 0 2324 0 2353 0 2405 0 2510 0 2597 0 2649 0 2675 0 COMMENT 2709 0 2738 0 2747 0 2756 0 2771 0 2793 0 2796 0 2802 0 2845 0 2849 COMMENT 0 2884 0 2907 0 2945 0 2963 0 3027 0 3161 0 3182 0 3194 0 3329 0 COMMENT 3368 0 3407 0 3481 0 3562 0 3732 0 3757 0 3828 0 3888 0 3909 0 3930 COMMENT 0 3992 0 4033 0 4069 0 4079 0 4109 0 4131 0 4197 0 4227 0 4455 0 COMMENT 4636 0 4668 0 4698 0 4766 0 4773 0 4783 0 4846 0 4885 0 4930 0 4942 COMMENT 0 5071 0 5146 0 5288 0 5293 0 5327 0 5363 0 5396 0 5539 0 5594 0 COMMENT 5621 0 5637 0 5667 0 5793 0 5852 0 5926 0 5963 0 6014 0 6040 0 6074 COMMENT 0 6128 0 6173 0 6317 0 6328 0 6334 0 6401 0 6410 0 6419 0 6460 0 0 COMMENT "") COMMENT #14=(CRSite (CStringList) "cat" "cat" 1 0 100 30 0 252 0 527 0 547 0 571 COMMENT 0 578 0 602 0 622 0 678 0 727 0 787 0 830 0 862 0 874 0 889 0 908 0 COMMENT 1009 0 1092 0 1096 0 1099 0 1139 0 1169 0 1174 0 1183 0 1186 0 1207 COMMENT 0 1231 0 1243 0 1279 0 1293 0 1303 0 1315 0 1319 0 1373 0 1422 0 COMMENT 1700 0 2142 0 2296 0 2319 0 2359 0 2404 0 2469 0 2509 0 2532 0 2568 COMMENT 0 2610 0 2674 0 2737 0 2801 0 2936 0 2959 0 3022 0 3026 0 3096 0 COMMENT 3160 0 3181 0 3292 0 3367 0 3385 0 3406 0 3457 0 3498 0 3557 0 3756 COMMENT 0 3836 0 3851 0 3872 0 3895 0 3902 0 3908 0 3929 0 4108 0 4150 0 COMMENT 4171 0 4201 0 4210 0 4304 0 4472 0 4561 0 4573 0 4597 0 4635 0 4640 COMMENT 0 4662 0 4710 0 4772 0 4821 0 4845 0 4877 0 5070 0 5447 0 5620 0 COMMENT 5962 0 6054 0 6127 0 6228 0 6370 0 6409 0 6459 0 6581 0 0 "") COMMENT #15=(CRSite (CStringList) "taa" "taa" 1 0 89 33 0 159 0 304 0 371 0 402 COMMENT 0 419 0 430 0 442 0 453 0 521 0 554 0 647 0 743 0 763 0 952 0 1046 COMMENT 0 1088 0 1114 0 1141 0 1180 0 1307 0 1331 0 1375 0 1401 0 1438 0 COMMENT 1520 0 1607 0 1715 0 1749 0 1794 0 1940 0 2178 0 2365 0 2561 0 2647 COMMENT 0 2664 0 2679 0 2720 0 2745 0 2830 0 2899 0 2961 0 3104 0 3111 0 COMMENT 3192 0 3372 0 3387 0 3479 0 3491 0 3501 0 3560 0 3740 0 3753 0 3826 COMMENT 0 4031 0 4043 0 4120 0 4160 0 4225 0 4348 0 4458 0 4548 0 4580 0 COMMENT 4984 0 5018 0 5038 0 5048 0 5054 0 5210 0 5253 0 5322 0 5352 0 5406 COMMENT 0 5484 0 5568 0 5676 0 5724 0 5976 0 6012 0 6069 0 6117 0 6204 0 COMMENT 6216 0 6305 0 6484 0 6525 0 6574 0 6583 0 6600 0 0 "") COMMENT #16=(CRSite (CStringList) "tta" "tta" 1 0 84 32 0 54 0 176 0 189 0 197 0 COMMENT 226 0 303 0 368 0 401 0 418 0 429 0 441 0 452 0 459 0 512 0 553 0 COMMENT 560 0 597 0 612 0 742 0 768 0 825 0 864 0 903 0 951 0 963 0 1159 0 COMMENT 1281 0 1296 0 1330 0 1389 0 1396 0 1414 0 1437 0 1663 0 1723 0 1760 COMMENT 0 1786 0 2004 0 2107 0 2158 0 2186 0 2291 0 2364 0 2476 0 2646 0 COMMENT 2678 0 2787 0 2810 0 2898 0 2998 0 3495 0 3500 0 3559 0 3739 0 3990 COMMENT 0 4030 0 4173 0 4224 0 4275 0 4347 0 4585 0 4696 0 4712 0 4983 0 COMMENT 5047 0 5053 0 5101 0 5107 0 5114 0 5183 0 5209 0 5246 0 5270 0 5309 COMMENT 0 5325 0 5351 0 5368 0 5375 0 5708 0 5723 0 5741 0 5835 0 6339 0 0 COMMENT "") COMMENT #17=(CRSite (CStringList) "tga" "tga" 1 0 126 66 0 223 0 232 0 260 0 282 COMMENT 0 364 0 409 0 414 0 549 0 585 0 630 0 650 0 776 0 789 0 793 0 800 0 COMMENT 809 0 914 0 924 0 1028 0 1084 0 1123 0 1165 0 1268 0 1362 0 1424 0 COMMENT 1443 0 1459 0 1498 0 1813 0 1868 0 1884 0 1891 0 2032 0 2037 0 2053 COMMENT 0 2166 0 2196 0 2200 0 2205 0 2249 0 2273 0 2351 0 2395 0 2437 0 COMMENT 2447 0 2490 0 2582 0 2628 0 2661 0 2707 0 2748 0 2757 0 2791 0 2794 COMMENT 0 2797 0 2824 0 2977 0 3091 0 3234 0 3245 0 3330 0 3369 0 3432 0 COMMENT 3441 0 3482 0 3486 0 3563 0 3632 0 3648 0 3672 0 3730 0 3758 0 3855 COMMENT 0 3918 0 3966 0 3972 0 3982 0 4062 0 4129 0 4228 0 4240 0 4332 0 COMMENT 4545 0 4609 0 4614 0 4736 0 4745 0 4847 0 4883 0 4928 0 5010 0 5072 COMMENT 0 5123 0 5217 0 5238 0 5279 0 5402 0 5624 0 5661 0 5772 0 5790 0 COMMENT 5814 0 5846 0 5864 0 5873 0 5916 0 6015 0 6041 0 6057 0 6065 0 6075 COMMENT 0 6099 0 6174 0 6240 0 6326 0 6332 0 6335 0 6431 0 6461 0 6479 0 COMMENT 6511 0 6546 0 6567 0 6611 0 6626 0 0 "") COMMENT #18=(CRSite (CStringList) "tca" "tca" 1 0 109 151 0 239 0 339 0 467 0 COMMENT 530 0 546 0 570 0 580 0 601 0 606 0 615 0 663 0 729 0 756 0 771 0 COMMENT 783 0 849 0 873 0 876 0 882 0 891 0 907 0 1011 0 1023 0 1032 0 1098 COMMENT 0 1101 0 1150 0 1173 0 1185 0 1213 0 1311 0 1372 0 1421 0 1465 0 COMMENT 1484 0 1578 0 1616 0 1676 0 1774 0 2062 0 2139 0 2245 0 2311 0 2335 COMMENT 0 2403 0 2515 0 2525 0 2531 0 2534 0 2567 0 2570 0 2591 0 2609 0 COMMENT 2703 0 2736 0 2775 0 2871 0 2875 0 2881 0 3025 0 3037 0 3070 0 3095 COMMENT 0 3141 0 3159 0 3291 0 3384 0 3405 0 3450 0 3515 0 3625 0 3658 0 COMMENT 3894 0 3898 0 4107 0 4143 0 4152 0 4209 0 4231 0 4408 0 4462 0 4525 COMMENT 0 4594 0 4600 0 4634 0 4844 0 4993 0 5172 0 5220 0 5391 0 5432 0 COMMENT 5449 0 5464 0 5474 0 5508 0 5520 0 5838 0 5860 0 5886 0 5987 0 6109 COMMENT 0 6126 0 6227 0 6233 0 6243 0 6293 0 6361 0 6381 0 0 "") COMMENT #19=(CRSite (CStringList) "tag" "tag" 1 0 40 22 0 90 0 177 0 190 0 227 0 COMMENT 244 0 263 0 307 0 561 0 680 0 1160 0 1254 0 1342 0 1475 0 1649 0 COMMENT 1654 0 1660 0 1664 0 1688 0 1783 0 2007 0 2361 0 2910 0 2926 0 3130 COMMENT 0 3402 0 4174 0 4221 0 4713 0 4728 0 4731 0 4897 0 4954 0 4999 0 COMMENT 5032 0 5247 0 5360 0 5942 0 6230 0 6578 0 0 "") COMMENT #20=(CRSite (CStringList) "cta" "cta" 1 0 49 72 0 89 0 158 0 345 0 355 0 COMMENT 394 0 504 0 520 0 739 0 1045 0 1197 0 1341 0 1552 0 1585 0 1648 0 COMMENT 1697 0 1714 0 1857 0 1876 0 1888 0 2077 0 2384 0 2390 0 3103 0 3129 COMMENT 0 3357 0 3478 0 3490 0 3787 0 3958 0 4042 0 4300 0 4631 0 4996 0 COMMENT 5031 0 5084 0 5394 0 5427 0 5481 0 5576 0 5640 0 5941 0 6203 0 6304 COMMENT 0 6453 0 6524 0 6577 0 6599 0 6638 0 0 "")) COMMENT (CObList COMMENT #21=(CFSignal (CObList) "T7 promoter" 21 0 0 4984 5002 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4984..5002") COMMENT #22=(CFSignal (CObList) "T7 terminator" 21 0 1 6521 6649 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(6525..6653)") COMMENT #23=(CFSignal (CObList) "ORF frame 2" 4 0 1 560 1375 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(560..1375)") COMMENT #24=(CFSignal (CObList) "pBR322_origin" 4 0 0 1481 2100 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1481..2100") COMMENT #25=(CFSignal (CObList) "ORF frame 1" 4 0 1 4347 4847 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4347..4847)") COMMENT #26=(CFSignal (CObList) "BAP" 21 0 0 5074 6486 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5075..6490")) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CObList #27=(CORF "atg" "tga" 2 1 2518 2739 1836212584 0 0 0 30) COMMENT #28=(CORF "atg" "taa" 2 0 2738 3103 1868770914 0 0 0 30) COMMENT #29=(CORF "atg" "tag" 2 1 3106 3459 43554400 0 0 0 30) COMMENT #30=(CORF "atg" "taa" 2 0 3329 3478 1818321769 0 0 0 30) COMMENT #31=(CORF "atg" "taa" 1 1 563 1375 43554064 0 0 0 30) COMMENT #32=(CORF "atg" "taa" 1 1 2813 3028 1818321769 0 0 0 30) COMMENT #33=(CORF "atg" "tga" 1 0 3481 3729 1818321769 0 0 0 30) COMMENT #34=(CORF "atg" "tga" 1 1 3518 4474 1702060386 0 0 0 30) COMMENT #35=(CORF "atg" "tag" 1 0 4636 4896 43555072 0 0 0 30) COMMENT #36=(CORF "atg" "tga" 3 1 2142 2321 0 0 0 0 30) COMMENT #37=(CORF "atg" "tga" 3 1 2406 2612 43555072 0 0 0 30) COMMENT #38=(CORF "atg" "taa" 3 1 2901 3098 43554568 0 0 0 30) COMMENT #39=(CORF "atg" "taa" 3 1 3498 3758 43554176 0 0 0 30) COMMENT #40=(CORF "atg" "taa" 3 1 4350 4847 0 0 0 0 30) COMMENT #41=(CORF "atg" "tga" 3 0 4773 5009 1868770914 0 0 0 30) COMMENT #42=(CORF "atg" "taa" 1 0 5071 6483 1868770914 0 0 0 30) COMMENT #43=(CORF "atg" "tag" 2 1 4045 4212 0 0 0 0 30) COMMENT #44=(CORF "atg" "tga" 2 1 5467 5622 1868770914 0 0 0 30) COMMENT #45=(CORF "atg" "tag" 2 0 6074 6229 1818321769 0 0 0 30) COMMENT #46=(CORF "atg" "tga" 3 1 5889 6056 1818321769 0 0 0 30)) COMMENT (CTextView 0 COMMENT #47=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #48=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "N_pET28-N-BAP" 2) COMMENT 5 "" 0 4) COMMENT #49=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'N_pET28-N-BAP'" 1) COMMENT #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 06/14/22 04:06\277\300\310\304" 1) COMMENT #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 06/14/22 04:06\277\300\310\304" 1) COMMENT #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6649 bp" 1) COMMENT #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #57=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 6-APR-2022" 1))) COMMENT "Standard Fields") COMMENT #60=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #61=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #62=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #63=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #64=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 560 (3 #23# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 560 End: 1375 (Complementary)" COMMENT 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(560..1375)" 1))) COMMENT "ORF frame 2") COMMENT #68=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1481 (3 #24# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1481 End: 2100" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1481..2100" 1))) "pBR322_origin") COMMENT #72=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4347 (3 #25# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4347 End: 4847 (Complementary)" COMMENT 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4347..4847)" 1))) COMMENT "ORF frame 1"))) "CDS (3 total)") COMMENT #76=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #77=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4984 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4984 End: 5002" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4984..5002" 1))) "T7 promoter") COMMENT #81=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5074 (3 #26# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5074 End: 6486" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5075..6490" 1))) "BAP") COMMENT #85=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6521 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6521 End: 6649 (Complementary)" COMMENT 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(6525..6653)" 1))) COMMENT "T7 terminator"))) "Misc. Feature (3 total)"))) COMMENT "Feature Map") COMMENT #89=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #90=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43546080 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #91=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #92=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 1068 " 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 6488 " 1))))) "AvaI: 2 sites") 1 5 COMMENT "CYCGRG" "GRGCYC") COMMENT #94=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545504 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #95=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545216 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #96=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 5866 " 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 6197 " 1))))) "EcoRI: 2 sites") 1 COMMENT 5 "GAATTC" "CTTAAG") COMMENT #99=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545248 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" COMMENT "TTCGAA") COMMENT #100=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545600 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #101=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #102=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 5962 " 1))))) "NcoI: 1 site") 1 COMMENT 5 "CCATGG" "GGTACC") COMMENT #103=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545728 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NdeI: 0 sites") 2 4 "CATATG" COMMENT "GTATAC") COMMENT #104=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43545664 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" COMMENT "CGATCG") COMMENT #105=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43524320 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" COMMENT "CGCCGGCG") COMMENT #106=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43524608 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SacI: 0 sites") 5 1 "GAGCTC" COMMENT "CTCGAG") COMMENT #107=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43523936 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SalI: 0 sites") 1 5 "GTCGAC" COMMENT "CAGCTG") COMMENT #108=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43523968 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #109=(CGroupPar COMMENT (CParagraph 0 (10 #10# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (1 #10# 1) 1 2 2 0 191) COMMENT " N1: 1070 " 1))))) "SmaI: 1 site") 3 COMMENT 3 "CCCGGG" "GGGCCC") COMMENT #111=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43524512 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #112=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #113=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT " N1: 6488 " 1))))) "XhoI: 1 site") 1 COMMENT 5 "CTCGAG" "GAGCTC") COMMENT #114=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43524640 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #115=(CGroupPar COMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #116=(CLinePar COMMENT (CParagraph 0 (1 #12# 1) 1 2 2 0 191) COMMENT " N1: 1068 " 1))))) "XmaI: 1 site") 1 COMMENT 5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map") COMMENT #117=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #118=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #119=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #120=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554944 (4 #33# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #122=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3481 - 3729" COMMENT 1))) "3481(d1) (83 codons)") COMMENT #123=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554888 (4 #35# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #125=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4636 - 4896" COMMENT 1))) "4636(d1) (87 codons)") COMMENT #126=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555056 (4 #42# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5071 - 6483" COMMENT 1))) "5071(d1) (471 codons)"))) COMMENT "Phase #1 (3 frames)") COMMENT #129=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #130=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554160 (4 #28# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2738 - 3103" COMMENT 1))) "2738(d2) (122 codons)") COMMENT #133=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554328 (4 #30# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #134=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #135=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3329 - 3478" COMMENT 1))) "3329(d2) (50 codons)") COMMENT #136=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555336 (4 #45# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #137=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6074 - 6229" COMMENT 1))) "6074(d2) (52 codons)"))) COMMENT "Phase #2 (3 frames)") COMMENT #139=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #140=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554384 (4 #41# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #141=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #142=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4773 - 5009" COMMENT 1))) "4773(d3) (79 codons)"))) COMMENT "Phase #3 (1 frame)"))) "Direct strand") COMMENT #143=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #144=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 -1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #145=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554776 (4 #31# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #147=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 563 - 1375" COMMENT 1))) "563(c1) (271 codons)") COMMENT #148=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554664 (4 #32# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #149=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #150=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2813 - 3028" COMMENT 1))) "2813(c1) (72 codons)") COMMENT #151=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555224 (4 #34# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #152=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #153=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3518 - 4474" COMMENT 1))) "3518(c1) (319 codons)"))) COMMENT "Phase #1 (3 frames)") COMMENT #154=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 -2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #155=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555112 (4 #27# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #156=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #157=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2518 - 2739" COMMENT 1))) "2518(c2) (74 codons)") COMMENT #158=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555672 (4 #29# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #159=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #160=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3106 - 3459" COMMENT 1))) "3106(c2) (118 codons)") COMMENT #161=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554104 (4 #43# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #162=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #163=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4045 - 4212" COMMENT 1))) "4045(c2) (56 codons)") COMMENT #164=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554496 (4 #44# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #165=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #166=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5467 - 5622" COMMENT 1))) "5467(c2) (52 codons)"))) COMMENT "Phase #2 (4 frames)") COMMENT #167=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 -3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #168=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554720 (4 #36# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #169=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #170=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2142 - 2321" COMMENT 1))) "2142(c3) (60 codons)") COMMENT #171=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554048 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #172=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #173=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2406 - 2612" COMMENT 1))) "2406(c3) (69 codons)") COMMENT #174=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555728 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #175=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #176=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2901 - 3098" COMMENT 1))) "2901(c3) (66 codons)") COMMENT #177=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43554552 (4 #39# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #178=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #179=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3498 - 3758" COMMENT 1))) "3498(c3) (87 codons)") COMMENT #180=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555616 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #181=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #182=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4350 - 4847" COMMENT 1))) "4350(c3) (166 codons)") COMMENT #183=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 43555392 (4 #46# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #184=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #185=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5889 - 6056" COMMENT 1))) "5889(c3) (56 codons)"))) COMMENT "Phase #3 (6 frames)"))) COMMENT "Complementary strand"))) "Open Reading Frames")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #186=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #187=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #188=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #189=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #190=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #191=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #192=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #193=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #194=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #195=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #196=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #197=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #198=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #199=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #200=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #201=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #202=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #203=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #204=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #205=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #206=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #207=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #208=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #209=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #210=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #211=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #212=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #213=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #214=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #215=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #216=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #217=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #218=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #219=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #220=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #221=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #222=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #223=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #224=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #225=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -5369) 1 1 0 1 1 COMMENT (mapper: 23.8082 -23.2286 40.0378 40.0378 0.01 10 -1 6649 6649 1 0 0) COMMENT #226=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 541412933 100) COMMENT (CObjectList COMMENT #227=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 538976288 100) COMMENT (CObjectList COMMENT #228=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #192# 52681140 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27513 6.27313 1 0.0214037) 0.0943478) COMMENT #229=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #189# 2 100) COMMENT 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COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Eco}RI (6197)" "@N (@S)" 5 -10.0828 COMMENT 15.681 0 -10 5.31997 0.949103 #296#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #298=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1161 100) COMMENT (CObjectList) (CObjectList)) COMMENT #299=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -922 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 0 COMMENT (CObList #300=(AnlzFontBackColorItem 5068 5072 42572451) COMMENT #301=(AnlzFontBackColorItem 6486 6491 46186688) COMMENT #302=(AnlzFontBackColorItem 5074 6485 40304639) COMMENT #303=(AnlzFontBackColorItem 5073 5073 40304639))) (CObList) 2081903016 COMMENT (CStringList) 2081903016 0 (CObList))) FEATURES Location/Qualifiers misc_feature 4984..5002 /vntifkey="21" /label=T7\promoter misc_feature complement(6521..6649) /vntifkey="21" /label=T7\terminator CDS complement(560..1375) /vntifkey="4" /label=ORF\frame\2 CDS 1481..2100 /vntifkey="4" /label=pBR322_origin CDS complement(4347..4847) /vntifkey="4" /label=ORF\frame\1 misc_feature 5074..6486 /vntifkey="21" /label=BAP BASE COUNT 1612 a 1772 c 1735 g 1530 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgaaacaaa gcactattgc actggcactc 5101 ttaccgttac tgtttacccc tgtgacaaaa gcccggacac cagaaatgcc tgttctggaa 5161 aaccgggctg ctcagggcga tattactgca cccggcggtg ctcgccgttt aacgggtgat 5221 cagactgccg ctctgcgtga ttctcttagc gataaacctg caaaaaatat tattttgctg 5281 attggcgatg ggatggggga ctcggaaatt actgccgcac gtaattatgc cgaaggtgcg 5341 ggcggctttt ttaaaggtat agatgcctta ccgcttaccg ggcaatacac tcactatgcg 5401 ctgaataaaa aaaccggcaa accggactac gtcaccgact cggctgcatc agcaaccgcc 5461 tggtcaaccg gtgtcaaaac ctataacggc gcgctgggcg tcgatattca cgaaaaagat 5521 cacccaacga ttctggaaat ggcaaaagcc gcaggtctgg cgaccggtaa cgtttctacc 5581 gcagagttgc aggatgccac gcccgctgcg ctggtggcac atgtgacctc gcgcaaatgc 5641 tacggtccga gcgcgaccag tgaaaaatgt ccgggtaacg ctctggaaaa aggcggaaaa 5701 ggatcgatta ccgaacagct gcttaacgct cgtgccgacg ttacgcttgg cggcggcgca 5761 aaaacctttg ctgaaacggc aaccgctggt gaatggcagg gaaaaacgct gcgtgaacag 5821 gcacaggcgc gtggttatca gttggtgagc gatgctgcct cactgaattc ggtgacggaa 5881 gcgaatcagc aaaaacccct gcttggcctg tttgctgacg gcaatatgcc agtgcgctgg 5941 ctaggaccga aagcaacgta ccatggcaat atcgataagc ccgcagtcac ctgtacgcca 6001 aatccgcaac gtaatgacag tgtaccaacc ctggcgcaga tgaccgacaa agccattgaa 6061 ttgttgagta aaaatgagaa aggctttttc ctgcaagttg aaggtgcgtc aatcgataaa 6121 caggatcatg ctgcgaatcc ttgtgggcaa attggcgaga cggtcgatct cgatgaagcc 6181 gtacaacggg cgctggaatt cgctaaaaag gagggtaaca cgctggtcat agtcaccgct 6241 gatcacgccc acgccagcca gattgttgcg ccggatacca aagctccggg cctcacccag 6301 gcgctaaata ccaaagatgg cgcagtgatg gtgatgagtt acgggaactc cgaagaggat 6361 tcacaagaac ataccggcag tcagttgcgt attgcggcgt atggcccgca tgccgccaat 6421 gttgttggac tgaccgacca gaccgatctc ttctacacca tgaaagccgc tctggggctg 6481 aaataactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag 6541 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct 6601 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat //