LOCUS RC2204-09\pET28a 6162 bp DNA linear 3-JUN-2024 DEFINITION Complementary copy of 210617 pET-28. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-15473809| COMMENT VNTDBDATE|-15473809| COMMENT LSOWNER| COMMENT VNTNAME|RC2204-09 pET28a-LacUV5-EGFP (ctrl)| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "RC2204-09 pET28a-LacUV5-EGFP (ctrl)" 1 6162 COMMENT (CDBMol 1 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "NcoI" "NdeI" "XhoI" "BglII") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6162 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5147 0 0 "") COMMENT #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 5084 0 0 "") COMMENT #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 6001 0 0 "") COMMENT #3=(CRSite (CStringList) "BglII" "agatct" 2 0 1 4965 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "ORF frame 2" 4 0 1 560 1375 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(560..1375)") COMMENT #5=(CFSignal (CObList) "pBR322_origin" 33 0 0 1481 2100 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1481..2100") COMMENT #6=(CFSignal (CObList) "pGEX_3_primer" 21 0 0 2499 2521 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2499..2521") COMMENT #7=(CFSignal (CObList) "ROP" 21 0 1 2515 2706 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2515..2706)") COMMENT #8=(CFSignal (CObList) "ORF frame 2" 4 0 1 3515 4474 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3515..4474)") COMMENT #9=(CFSignal (CObList) "lacI" 21 0 1 3515 4606 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3515..4606)") COMMENT #10=(CFSignal (CObList) "ORF frame 1" 4 0 1 4347 4847 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4347..4847)") COMMENT #11=(CFSignal (CObList) "pBRrevBam_primer" 21 0 0 4881 4900 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4881..4900") COMMENT #12=(CFSignal (CObList) "Xpress_fwd_primer" 21 0 0 5927 5941 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5930..5944") COMMENT #13=(CFSignal (CObList) "T7_leader" 21 0 0 5927 5956 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5930..5959") COMMENT #14=(CFSignal (CObList) "T7_Terminal_primer" 21 0 1 6076 6094 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(6079..6097)") COMMENT #15=(CFSignal (CObList) "T7_terminator" 43 0 0 6034 6162 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6037..6165") COMMENT #16=(CFSignal (CObList) "LacUV5" 21 0 0 4970 5926 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4970..5929")) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #18=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "RC2204-09 pET28a-LacUV5-EGFP (ctrl)" 2) 5 "" 0 4) COMMENT #19=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'RC2204-09 pET28a-LacUV5-EGFP (ctrl)'" 1) COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Complementary copy of 210617 pET-28." 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 06/03/24 03:04\277\300\310\304" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 06/03/24 03:04\277\300\310\304" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6162 bp" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Linear" 1))) "General Description") COMMENT #28=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 2-JUN-2022" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: KanR2; tet (576 - 851); tet (300 - 563)" COMMENT 1))) "Standard Fields") COMMENT #32=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #36=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 560 (3 #4# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #37=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 560 End: 1375 (Complementary)" COMMENT 1) COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(560..1375)" 1))) COMMENT "ORF frame 2") COMMENT #40=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3515 (3 #8# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3515 End: 4474 (Complementary)" COMMENT 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3515..4474)" 1))) COMMENT "ORF frame 2") COMMENT #44=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4347 (3 #10# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4347 End: 4847 (Complementary)" COMMENT 1) COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4347..4847)" 1))) COMMENT "ORF frame 1"))) "CDS (3 total)") COMMENT #48=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2499 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2499 End: 2521" 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2499..2521" 1))) "pGEX_3_primer") COMMENT #53=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2515 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2515 End: 2706 (Complementary)" COMMENT 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2515..2706)" 1))) "ROP") COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3515 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3515 End: 4606 (Complementary)" COMMENT 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3515..4606)" 1))) "lacI") COMMENT #61=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4881 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4881 End: 4900" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4881..4900" 1))) "pBRrevBam_primer") COMMENT #65=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4970 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4970 End: 5926" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4970..5929" 1))) "LacUV5") COMMENT #69=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5927 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5927 End: 5941" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5930..5944" 1))) "Xpress_fwd_primer") COMMENT #73=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5927 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5927 End: 5956" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5930..5959" 1))) "T7_leader") COMMENT #77=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6076 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6076 End: 6094 (Complementary)" COMMENT 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(6079..6097)" 1))) COMMENT "T7_Terminal_primer"))) COMMENT "Misc. Feature (8 total)") COMMENT #81=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #82=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1481 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1481 End: 2100" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1481..2100" 1))) "pBR322_origin"))) COMMENT "Replication Origin (1 total)") COMMENT #86=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #87=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6034 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6034 End: 6162" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 6037..6165" 1))) "T7_terminator"))) COMMENT "Terminator (1 total)"))) "Feature Map") COMMENT #91=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #92=(CFolderPar COMMENT (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #93=(CFolderPar COMMENT (CGroupPar (CParagraph 560 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 560 End: 1375 (Complementary)" COMMENT 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(560..1375)" 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"KanR2\"" 1))) "KanR2") COMMENT #99=(CFolderPar COMMENT (CGroupPar (CParagraph 3181 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3181 End: 3456 (Complementary)" COMMENT 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3181..3456)" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (576 - 851)\"" 1))) COMMENT "tet (576 - 851)") COMMENT #105=(CFolderPar COMMENT (CGroupPar (CParagraph 4689 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4689 End: 4952 (Complementary)" COMMENT 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4689..4952)" 1) COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (300 - 563)\"" 1))) COMMENT "tet (300 - 563)"))) "Gene (3 total)") COMMENT #111=(CFolderPar COMMENT (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #112=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #113=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 6162 (Complementary)" COMMENT 1) COMMENT #114=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1..6162)" 1) COMMENT #116=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /organism=\"pET-28 a (+)\"" 1) COMMENT #118=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /mol_type=\"other DNA\"" 1))) COMMENT "Source_1"))) "Source (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #119=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #120=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43123840 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #121=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #122=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 4965 " 1))))) "BglII: 1 site") COMMENT 1 5 "AGATCT" "TCTAGA") COMMENT #123=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43137088 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #124=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #125=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 5147 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #126=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43137248 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #127=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #128=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 5084 " 1))))) "NdeI: 1 site") COMMENT 2 4 "CATATG" "GTATAC") COMMENT #129=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 43137312 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #130=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #131=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 6001 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #132=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #133=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #134=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #135=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #136=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #137=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #138=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #139=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #140=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #141=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #142=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #143=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #144=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #145=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #146=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #147=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #148=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #149=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #150=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #151=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #152=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #153=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #154=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #155=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #156=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #157=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #158=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #159=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #160=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #161=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #162=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #163=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #164=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #165=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #166=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #167=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #168=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #169=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #170=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #171=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -6162) 1 0 1 0 0 COMMENT (mapper: 16.2759 -18.1362 39 39 0.01 10 14 6162 6162 1 0 1) COMMENT #172=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #173=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #174=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #138# 90544092 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.2752 6.2732 1 0.0214037) 0.101804) COMMENT #175=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #135# 1953784679 100) COMMENT (LOGPEN 1634165603 1734566241 1734833255) 1734829923 COMMENT (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 2.53336 0.833333 0 COMMENT "RC2204-09 pET28a-LacUV5-EGFP (ctrl)" "@N" 1 0 0.5 0 COMMENT -6.206 14.6923 0.974359 Nil) COMMENT #176=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #134# 1634885968 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 2.53336 0.555556 0 "6162 bp" "@L bp" 16 0 -0.8 0 COMMENT -7.47268 2 0.615385 Nil)) (CObjectList)) COMMENT #177=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 14473084 100) COMMENT (CObjectList COMMENT #178=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil 2081903016 100) COMMENT (CObjectList COMMENT #179=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #136# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 4.87338 5.7041 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #180=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1953784167 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "ORF frame 2" COMMENT "@N" 1 0 0 -12.3346 1.97573 4.28205 0.820513 COMMENT #179#) COMMENT #181=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #136# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 1.71846 2.69578 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #182=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 959651897 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "ORF frame 2" COMMENT "@N" 1 0 0 6.47637 15.9092 4.28205 0.820513 COMMENT #181#) COMMENT #183=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #136# COMMENT 1667321128 100) (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 0.835356 1.33873 COMMENT 1.84877 1 0.082322) 0.8 1.8 1) COMMENT #184=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1667331171 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "ORF frame 1" COMMENT "@N" 1 0 0 9.29677 14.6425 4.28205 0.820513 COMMENT #183#)) (CObjectList)) COMMENT #185=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil 14473084 100) COMMENT (CObjectList COMMENT #186=(CScratch COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #141# 46380192 COMMENT 100) (LOGPEN 0 6 13395507) 10 1 3.73011 1.9 COMMENT 0.082322 1) COMMENT #187=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1952539508 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "pGEX 3 primer" COMMENT "@N" 1 0 0 -5.99453 4.50909 5.02564 0.820513 COMMENT #186#) COMMENT #188=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #137# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.51836 COMMENT 3.71382 1 0.082322) 0.8 1.8 1) COMMENT #189=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1952932195 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "ROP" "@N" 1 0 0 COMMENT -3.74618 5.77577 1.5641 0.820513 #188#) COMMENT #190=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #137# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 1.58408 COMMENT 2.69578 1 0.082322) 0.8 1.8 1) COMMENT #191=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1634165603 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "lacI" "@N" 1 0 COMMENT 0 5.27251 1.97573 1.30769 0.820513 #190#) COMMENT #192=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #141# 0 100) COMMENT (LOGPEN 0 6 13395507) 10 1 1.30513 1.9 COMMENT 0.082322 1) COMMENT #193=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1734828385 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 COMMENT "pBRrevBam primer" "@N" 1 0 0 11.7905 13.3758 COMMENT 6.5641 0.820513 #192#) COMMENT #194=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #141# 0 100) COMMENT (LOGPEN 0 6 13395507) 10 1 0.240258 1.9 COMMENT 0.082322 1) COMMENT #195=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 33023 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 COMMENT "Xpress fwd primer" "@N" 1 0 0 16.5717 COMMENT 5.77577 6.35897 0.820513 #194#) COMMENT #196=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #141# 46197432 COMMENT 100) (LOGPEN 0 6 13395507) 10 1 0.240258 1.9 COMMENT 0.082322 1) COMMENT #197=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1667327860 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "T7 leader" "@N" COMMENT 1 0 0 14.9563 7.04245 3.15385 0.820513 #196#) COMMENT #198=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #141# 46379800 COMMENT 100) (LOGPEN 0 6 13395507) 10 1 0.0885698 COMMENT 1.9 0.082322 1) COMMENT #199=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1734571105 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 COMMENT "T7 Terminal primer" "@N" 1 0 0 17.2691 COMMENT 3.24241 6.35897 0.820513 #198#) COMMENT #200=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #137# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.240258 COMMENT 1.21453 1 0.082322) 0.8 1.8 0) COMMENT #201=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1633772387 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "LacUV5" "@N" 1 COMMENT 0 0 12.4727 8.30913 2.69231 0.820513 #200#)) COMMENT (CObjectList)) COMMENT #202=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil 1948262459 100) COMMENT (CObjectList COMMENT #203=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #136# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 4.13529 4.76648 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #204=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1953784180 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "pBR322 origin" COMMENT "@N" 1 0 0 -9.00174 3.24241 4.82051 0.820513 COMMENT #203#)) (CObjectList)) COMMENT #205=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil 1948262459 100) COMMENT (CObjectList COMMENT #206=(CLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #141# 808722480 COMMENT 100) (LOGPEN 0 6 13395507) 10 0.835356 0 COMMENT 0.131328) COMMENT #207=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #133# 1953784161 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 2.53336 0.666667 0 "T7 terminator" COMMENT "@N" 1 0 0 16.4258 1.97573 4.48718 0.820513 COMMENT #206#)) (CObjectList))) (CObjectList)) COMMENT #208=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #209=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 1634297714 100) COMMENT (CObjectList COMMENT #210=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #140# 46102808 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.03433 1.9 COMMENT 0.082322 1) COMMENT #211=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #142# 1633773415 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Nco}I (5147)" "@N (@S)" 5 0 0 11.1896 COMMENT 9.57581 2.94872 0.615385 #210#)) COMMENT (CObjectList)) COMMENT #212=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #213=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #140# 46103832 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.09847 1.9 COMMENT 0.082322 1) COMMENT #214=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #142# 1635018599 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Nde}I (5084)" "@N (@S)" 5 0 0 11.0357 COMMENT 10.8425 3.20513 0.615385 #213#)) COMMENT (CObjectList)) COMMENT #215=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 2081903016 100) COMMENT (CObjectList COMMENT #216=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #140# 46105240 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.164923 1.9 COMMENT 0.082322 1) COMMENT #217=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #142# 1668573027 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Xho}I (6001)" "@N (@S)" 5 0 0 15.2899 COMMENT 4.50909 3.12821 0.615385 #216#)) COMMENT (CObjectList)) COMMENT #218=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil 1948262459 100) COMMENT (CObjectList COMMENT #219=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #140# 46104216 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.21962 1.9 COMMENT 0.082322 1) COMMENT #220=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #142# 10297 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 27 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 2.53336 0.555556 128 COMMENT "{\\i Bgl}II (4965)" "@N (@S)" 5 0 0 10.4786 COMMENT 12.1092 3.20513 0.615385 #219#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #221=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 2 100) COMMENT (CObjectList) (CObjectList)) COMMENT #222=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 43301264 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList #223=(XLATFRAGMENT 5084 279 0 1 5085 839)) COMMENT (CObList #224=(AnlzFontColorItem 4969 5129 33554432) COMMENT #225=(AnlzFontColorItem 5939 6025 33554432) COMMENT #226=(AnlzFontColorItem 5162 5908 33554432) COMMENT #227=(AnlzFontColorItem 5130 5131 33554432) COMMENT #228=(AnlzFontColorItem 5909 5938 37173247) COMMENT #229=(AnlzFontColorItem 5132 5161 37173247) COMMENT #230=(AnlzXLATItem 5084 5923 3 5084 5923)) 1 COMMENT (CObList #231=(AnlzFontBackColorItem 4963 4968 46186688) COMMENT #232=(AnlzFontBackColorItem 4969 5144 40304639) COMMENT #233=(AnlzFontBackColorItem 5924 5925 40304639) COMMENT #234=(AnlzFontBackColorItem 5147 5923 50318387) COMMENT #235=(AnlzFontBackColorItem 5145 5146 46186688))) (CObList) 90056192 COMMENT (CStringList) 95712104 3980107682 (CObList))) FEATURES Location/Qualifiers CDS complement(560..1375) /vntifkey="4" /label=ORF\frame\2 gene complement(560..1375) /gene="KanR2" /vntifkey="60" rep_origin 1481..2100 /vntifkey="33" /label=pBR322_origin misc_feature 2499..2521 /vntifkey="21" /label=pGEX_3_primer misc_feature complement(2515..2706) /vntifkey="21" /label=ROP gene complement(3181..3456) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3515..4474) /vntifkey="4" /label=ORF\frame\2 misc_feature complement(3515..4606) /vntifkey="21" /label=lacI CDS complement(4347..4847) /vntifkey="4" /label=ORF\frame\1 misc_feature 4881..4900 /vntifkey="21" /label=pBRrevBam_primer gene complement(4689..4952) /gene="tet (300 - 563)" /vntifkey="60" misc_feature 5927..5941 /vntifkey="21" /label=Xpress_fwd_primer misc_feature 5927..5956 /vntifkey="21" /label=T7_leader misc_feature complement(6076..6094) /vntifkey="21" /label=T7_Terminal_primer terminator 6034..6162 /vntifkey="43" /label=T7_terminator source complement(1..6162) /organism="pET-28 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 4970..5926 /vntifkey="21" /label=LacUV5 BASE COUNT 1460 a 1709 c 1606 g 1387 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctt cactcattag 4981 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggaat tgtgagcgga 5041 taacaatttc acacaggaaa cagctttaag aaggagatat acatatgggc agcagccatc 5101 atcatcatca tcacagcagc ggcgaaaacc tgtacttcca gagcgccatg ggccatcatc 5161 atcatcatca tcatcatcat cacagcagcg gccatatcga aggtcgtgtg agcaagggcg 5221 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 5281 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 5341 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 5401 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 5461 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 5521 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 5581 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 5641 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaacc 5701 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 5761 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 5821 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 5881 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagcgcta gcatgactgg 5941 tggacagcaa atgggtcgcg gatccgaatt cgagctccgt cgacaagctt gcggccgcac 6001 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg 6061 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg 6121 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at //