LOCUS RC2205-02\pET-28 5824 bp DNA linear 13-JUN-2022 DEFINITION Complementary copy of 210617 pET-28. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14396367| COMMENT VNTDBDATE|-14435557| COMMENT LSOWNER| COMMENT VNTNAME|RC2205-02 pET-28 rc+smt3-E-PPase(His)| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Feature (9 total)") COMMENT #124=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #125=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4984 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #126=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4984 End: 5002" 1) COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4984..5002" 1))) "T7_promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #129=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #130=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1481 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1481 End: 2100" 1) COMMENT #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #133=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1481..2100" 1))) "pBR322_origin"))) COMMENT "Replication Origin (1 total)") COMMENT #134=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #135=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5696 (3 #27# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #136=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5696 End: 5824" 1) COMMENT #137=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5696..5824" 1))) "T7_terminator"))) COMMENT "Terminator (1 total)"))) "Feature Map") COMMENT #139=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #140=(CFolderPar COMMENT (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #141=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 560 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #142=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 560 End: 1375 (Complementary)" COMMENT 1) COMMENT #143=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #144=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(560..1375)" 1) COMMENT #145=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"KanR2\"" 1))) "KanR2") COMMENT #147=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3181 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #148=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3181 End: 3456 (Complementary)" COMMENT 1) COMMENT #149=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #150=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3181..3456)" 1) COMMENT #151=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #152=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (576 - 851)\"" 1))) COMMENT "tet (576 - 851)") COMMENT #153=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4689 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #154=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4689 End: 4952 (Complementary)" COMMENT 1) COMMENT #155=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #156=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4689..4952)" 1) COMMENT #157=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #158=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (300 - 563)\"" 1))) COMMENT "tet (300 - 563)"))) "Gene (3 total)") COMMENT #159=(CFolderPar COMMENT (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #160=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #161=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 5824 (Complementary)" COMMENT 1) COMMENT #162=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #163=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1..5824)" 1) COMMENT #164=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #165=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /organism=\"pET-28 a (+)\"" 1) COMMENT #166=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /mol_type=\"other DNA\"" 1))) COMMENT "Source_1"))) "Source (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #167=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #168=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 95529056 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" COMMENT "CCATGG") COMMENT #169=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 95528640 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #170=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #171=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 5070 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #172=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 95528704 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #173=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #174=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 5130 " 1))))) "NdeI: 1 site") COMMENT 2 4 "CATATG" "GTATAC") COMMENT #175=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 95528512 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #176=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #177=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 5663 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC"))) COMMENT "Restriction/Methylation Map") COMMENT #178=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #179=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #180=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #181=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054872 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #182=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #183=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3256 - 3729" COMMENT 1))) "3256(d1) (158 codons)") COMMENT #184=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053640 (4 #39# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #185=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #186=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4438 - 4608" COMMENT 1))) "4438(d1) (57 codons)") COMMENT #187=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053080 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #188=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #189=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4636 - 4896" COMMENT 1))) "4636(d1) (87 codons)") COMMENT #190=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054480 (4 #50# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #191=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #192=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5071 - 5658" COMMENT 1))) "5071(d1) (196 codons)"))) COMMENT "Phase #1 (4 frames)") COMMENT #193=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #194=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054368 (4 #29# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #195=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tag" COMMENT 1) COMMENT #196=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 47 - 226" 1))) COMMENT "47(d2) (60 codons)") COMMENT #197=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054928 (4 #31# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #198=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #199=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2738 - 3103" COMMENT 1))) "2738(d2) (122 codons)") COMMENT #200=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054984 (4 #33# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #201=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #202=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3329 - 3478" COMMENT 1))) "3329(d2) (50 codons)") COMMENT #203=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053304 (4 #52# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #204=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #205=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4262 - 4579" COMMENT 1))) "4262(d2) (106 codons)") COMMENT #206=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054312 (4 #56# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #207=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #208=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5603 - 5752" COMMENT 1))) "5603(d2) (50 codons)"))) COMMENT "Phase #2 (5 frames)") COMMENT #209=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #210=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053584 (4 #41# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #211=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tag" COMMENT 1) COMMENT #212=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1080 - 1253" COMMENT 1))) "1080(d3) (58 codons)") COMMENT #213=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054200 (4 #48# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #214=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #215=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4773 - 5009" COMMENT 1))) "4773(d3) (79 codons)"))) COMMENT "Phase #3 (2 frames)"))) "Direct strand") COMMENT #216=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #217=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 -1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #218=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053416 (4 #34# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #219=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #220=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 563 - 1375" COMMENT 1))) "563(c1) (271 codons)") COMMENT #221=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054144 (4 #35# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #222=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #223=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2813 - 3073" COMMENT 1))) "2813(c1) (87 codons)") COMMENT #224=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054704 (4 #36# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #225=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #226=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3098 - 3421" COMMENT 1))) "3098(c1) (108 codons)") COMMENT #227=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053864 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #228=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #229=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3518 - 4606" COMMENT 1))) "3518(c1) (363 codons)") COMMENT #230=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054760 (4 #49# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #231=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #232=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4847 - 5137" COMMENT 1))) "4847(c1) (97 codons)"))) COMMENT "Phase #1 (5 frames)") COMMENT #233=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 -2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #234=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053528 (4 #30# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #235=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #236=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2518 - 2874" COMMENT 1))) "2518(c2) (119 codons)") COMMENT #237=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053472 (4 #32# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #238=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #239=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3106 - 3459" COMMENT 1))) "3106(c2) (118 codons)") COMMENT #240=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053808 (4 #51# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #241=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #242=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4045 - 4212" COMMENT 1))) "4045(c2) (56 codons)") COMMENT #243=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47053752 (4 #53# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #244=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #245=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5137 - 5313" COMMENT 1))) "5137(c2) (59 codons)") COMMENT #246=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 47054648 (4 #54# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #247=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #248=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5320 - 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COMMENT #403=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -694 100) COMMENT (CObjectList) (CObjectList)) COMMENT #404=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -443 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList #405=(XLATFRAGMENT 5130 176 0 1 5131 530)) COMMENT (CObList #406=(AnlzFontColorItem 5115 5129 37173247) COMMENT #407=(AnlzXLATItem 5130 5660 1 5130 5660) COMMENT #408=(AnlzFontColorItem 5661 5675 37173247) COMMENT #409=(AnlzFontColorItem 5145 5645 33554432) COMMENT #410=(AnlzFontColorItem 5130 5144 37173247) COMMENT #411=(AnlzFontColorItem 5646 5660 37173247)) 1 COMMENT (CObList #412=(AnlzFontBackColorItem 5127 5129 46186688) COMMENT #413=(AnlzFontBackColorItem 5661 5666 46186688) COMMENT #414=(AnlzFontBackColorItem 5130 5660 40304639))) (CObList) 46028640 COMMENT (CStringList) 94689512 1386832007 (CObList))) FEATURES Location/Qualifiers CDS complement(560..1375) /vntifkey="4" /label=ORF\frame\2 gene complement(560..1375) /gene="KanR2" /vntifkey="60" rep_origin 1481..2100 /vntifkey="33" /label=pBR322_origin misc_feature 2499..2521 /vntifkey="21" /label=pGEX_3_primer misc_feature complement(2515..2706) /vntifkey="21" /label=ROP gene complement(3181..3456) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3515..4474) /vntifkey="4" /label=ORF\frame\2 misc_feature complement(3515..4606) /vntifkey="21" /label=lacI CDS complement(4347..4847) /vntifkey="4" /label=ORF\frame\1 misc_feature 4881..4900 /vntifkey="21" /label=pBRrevBam_primer gene complement(4689..4952) /gene="tet (300 - 563)" /vntifkey="60" promoter 4984..5002 /vntifkey="30" /label=T7_promoter misc_feature 5002..5029 /vntifkey="21" /label=lacO misc_feature 5047..5063 /vntifkey="21" /label=T7_transl_en_RBS misc_feature 5130..5130 /vntifkey="21" /label=Xpress_fwd_primer misc_feature complement(5738..5756) /vntifkey="21" /label=T7_Terminal_primer terminator 5696..5824 /vntifkey="43" /label=T7_terminator source complement(1..5824) /organism="pET-28 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 5131..5661 /vntifkey="21" /label=E-PPase BASE COUNT 1399 a 1537 c 1504 g 1384 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5101 agcagcggcc tggtgccgcg cggcagccat atgtcacttc tgaatgttcc ggcaggaaag 5161 gacttgccgg aagacattta cgtggtaatc gaaattcccg caaacgcgga cccgattaaa 5221 tatgagatcg acaaagaaag cggtgccctt tttgttgatc gctttatgag tacggcaatg 5281 ttttatcctt gcaattatgg ttatattaat cacaccttat cgttggacgg agatcccgtc 5341 gatgtgttgg tgccaacacc ctaccctttg caaccgggat ctgttatccg ctgtcgcccg 5401 gtaggggttt tgaaaatgac cgatgaggca ggggaagacg ctaaacttgt cgccgtcccc 5461 cattccaagt tgagcaagga atacgaccac attaaggacg taaatgatct tccggaatta 5521 ttgaaggcac aaatcgcgca cttctttgag cattataaag accttgagaa ggggaaatgg 5581 gtaaaggttg aaggctggga gaatgctgag gcggcaaaag ccgaaatcgt ggcgtccttc 5641 gagcgtgcga aaaacaaata actcgagcac caccaccacc accactgaga tccggctgct 5701 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa 5761 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc 5821 ggat //