LOCUS pET21a-YeiC 6309 bp DNA circular 10-JAN-2022 DEFINITION Complementary copy of pET-21a. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14213910| COMMENT VNTDBDATE|-14213910| COMMENT LSOWNER| COMMENT VNTNAME|pET21a-YeiC| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList COMMENT #197=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 1145 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #198=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97211568 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" COMMENT "GGGCCC") COMMENT #199=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97222096 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #200=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #201=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT " N1: 6148 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC") COMMENT #202=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 97211664 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" COMMENT "GGGCCC"))) "Restriction/Methylation Map") COMMENT #203=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #204=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #205=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #206=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392888 (4 #53# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #207=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #208=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4906 - 5142" COMMENT 1))) "4906(d1) (79 codons)"))) COMMENT "Phase #1 (1 frame)") COMMENT #209=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #210=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42611360 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #211=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #212=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 599 - 1456" COMMENT 1))) "599(d2) (286 codons)") COMMENT #213=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42390832 (4 #43# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #214=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #215=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3614 - 3862" COMMENT 1))) "3614(d2) (83 codons)") COMMENT #216=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 41985176 (4 #45# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #217=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #218=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4769 - 5029" COMMENT 1))) "4769(d2) (87 codons)"))) COMMENT "Phase #2 (3 frames)") COMMENT #219=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 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(CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #236=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1066 - 1329" COMMENT 1))) "1066(c1) (88 codons)") COMMENT #237=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392384 (4 #48# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #238=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #239=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2275 - 2454" COMMENT 1))) "2275(c1) (60 codons)") COMMENT #240=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42393616 (4 #49# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #241=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #242=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2539 - 2745" COMMENT 1))) "2539(c1) (69 codons)") COMMENT #243=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392664 (4 #50# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #244=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #245=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3034 - 3231" COMMENT 1))) "3034(c1) (66 codons)") COMMENT #246=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392104 (4 #51# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #247=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #248=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3631 - 3891" COMMENT 1))) "3631(c1) (87 codons)") COMMENT #249=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42393448 (4 #52# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #250=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" 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COMMENT (CGroupPar COMMENT (CParagraph 42392216 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #267=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #268=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2651 - 2872" COMMENT 1))) "2651(c3) (74 codons)") COMMENT #269=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392944 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #270=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #271=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3239 - 3592" COMMENT 1))) "3239(c3) (118 codons)") COMMENT #272=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 42392328 (4 #54# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #273=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #274=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4178 - 4345" COMMENT 1))) "4178(c3) (56 codons)"))) COMMENT "Phase #3 (3 frames)"))) COMMENT "Complementary strand"))) "Open Reading Frames")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #275=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #276=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #277=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #278=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #279=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #280=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #281=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #282=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #283=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #284=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #285=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #286=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #287=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 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"Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (4396)" "@N (@S)" 5 -18.5604 COMMENT -5.22699 0 -10 5.44485 0.949103 #413#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #415=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -533 100) COMMENT (CObjectList) (CObjectList)) COMMENT #416=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -809 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 0 COMMENT (CObList #417=(AnlzFontBackColorItem 5201 5203 42572451) COMMENT #418=(AnlzFontBackColorItem 6146 6151 46186688) COMMENT #419=(AnlzFontBackColorItem 5207 6145 40304639) COMMENT #420=(AnlzFontBackColorItem 5204 5206 40304639))) (CObList) 56036096 COMMENT (CStringList) 56523752 1125777886 (CObList))) FEATURES Location/Qualifiers promoter 529..557 /vntifkey="30" /label=AmpR_promoter CDS 599..1459 /vntifkey="4" /label=ORF\frame\2 gene 599..1459 /gene="Ampicillin" /vntifkey="60" rep_origin 1614..2233 /vntifkey="33" /label=pBR322_origin misc_feature 2632..2654 /vntifkey="21" /label=pGEX_3_primer misc_feature complement(2648..2839) /vntifkey="21" /label=ROP gene complement(3314..3589) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3648..4607) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(3648..4739) /vntifkey="21" /label=lacI CDS complement(4480..4980) /vntifkey="4" /label=ORF\frame\2 misc_feature 5014..5033 /vntifkey="21" /label=pBRrevBam_primer gene complement(4822..5085) /gene="tet (300 - 563)" /vntifkey="60" promoter 5117..5135 /vntifkey="30" /label=T7_promoter misc_feature 5135..5162 /vntifkey="21" /label=lacO misc_feature 5180..5196 /vntifkey="21" /label=T7_transl_en_RBS misc_feature 5204..6146 /vntifkey="21" /label=Xpress_fwd_primer misc_feature 5205..6146 /vntifkey="21" /label=T7_leader misc_feature complement(6223..6241) /vntifkey="21" /label=T7_Terminal_primer terminator 6181..6309 /vntifkey="43" /label=T7_terminator source complement(1..6309) /organism="pET-21 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 5208..6146 /vntifkey="21" /label=YeiC BASE COUNT 1504 a 1616 c 1669 g 1520 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcgc gaaaaggatt 5221 atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat gaatcattaa 5281 attatgcgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta gggcgcaata 5341 ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc gtaggcagtg 5401 atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggcgtttat gtcgataaat 5461 gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat aataccggtg 5521 aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct gaatatctcg 5581 cacagcacgg tgaatttatt cagagggcaa aggtcattgt cgcggactgt aatatcagtg 5641 aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt gtcgatccgg 5701 tttcggcatg gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac actctcaagc 5761 caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt gaagatgtgg 5821 caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg agcatgggcg 5881 gcgacggcgt ttattacagc gatatcagcg gtgaaagtgg ctggtctgcg ccgatcaaaa 5941 ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt 6001 gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg 6061 cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt atatcgttag 6121 tggagaacgc agaatgtctg aattaactcg agcaccacca ccaccaccac tgagatccgg 6181 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag 6241 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta 6301 tatccggat //