LOCUS CS16-087-07-pET2 9339 bp DNA circular 31-OCT-2016 DEFINITION Complementary copy of pET21a. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11481898| COMMENT VNTDBDATE|-11481898| COMMENT LSOWNER| COMMENT VNTNAME|CS16-087-07-pET21a-O-Glycosidsase| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers promoter 529..557 /vntifkey="30" /label=AmpR_promoter CDS 599..1459 /vntifkey="4" /label=ORF\frame\2 gene 599..1459 /gene="Ampicillin" /vntifkey="60" rep_origin 1614..2233 /vntifkey="33" /label=pBR322_origin misc_feature 2632..2654 /vntifkey="21" /label=pGEX_3_primer misc_feature complement(2648..2839) /vntifkey="21" /label=ROP gene complement(3314..3589) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3648..4607) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(3648..4739) /vntifkey="21" /label=lacI CDS complement(4480..4980) /vntifkey="4" /label=ORF\frame\2 misc_feature 5014..5033 /vntifkey="21" /label=pBRrevBam_primer gene complement(4822..5085) /gene="tet (300 - 563)" /vntifkey="60" promoter 5117..5135 /vntifkey="30" /label=T7_promoter misc_feature 5135..5162 /vntifkey="21" /label=lacO misc_feature 5180..5196 /vntifkey="21" /label=T7_transl_en_RBS misc_feature 5204..5204 /vntifkey="21" /label=Xpress_fwd_primer misc_feature complement(9253..9271) /vntifkey="21" /label=T7_Terminal_primer terminator 9211..9339 /vntifkey="43" /label=T7_terminator source complement(1..9339) /organism="pET-21 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 5205..9176 /vntifkey="21" /label=O-Glycosidsase BASE COUNT 2536 a 2225 c 2430 g 2148 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc 5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa cacggtaaaa 5221 tcaagagatt ctccaccctg acccttttag catctgcaac catattggtc cccctgtcta 5281 ccagcgcgga agaaacaacg aatagctcga ccgagacctc aagtagcatg gtagagccaa 5341 cggcaactga agaaaagtta tggcaatccg attttccagg gggtaaaacg ggagagtggc 5401 aggatgttat aggcaagacc aaccgggagc tggcgggcga atcattggcc atctcgcgtg 5461 atgcggccgc cggaaacaac gcagtgtctc tgaatttaga ttcaccaaaa cttgctgacg 5521 gggaggtcga gactaagttc aaatacaccg ctggaagtgg ccgtacgggg gtaattattc 5581 gggggaatac aaaggattca tgggtattcg taggatataa tgccaatggg aaatggctgg 5641 ttgagagtcc gaatagttgg aatgatagta taagcggccc aacgttgaac gaagatacaa 5701 attacttatt gaaagtgcgt tatgtagggg agaaaattac catttggtta aataccacgc 5761 ttatctacga aggggagccg gtattagcga acggagacaa aatacctacc gaggcgggtc 5821 atgttggtgt gcggttatgg tatgataaga aaatcgttaa ttacgactat ttcaagaacg 5881 gtcctgtaga tagcatccca gagattgtac cggaggtgac ccaaatcgca ccagtaaaag 5941 tattcacaaa gattggtgtg gcccctaaat tacctaagca agtaaaagtt acgtataata 6001 cggggaaaga ggcgaacgaa gcagtacgtt ggaatgaaat cgatcctgac gcatataagg 6061 aaccgggaac ctttgaggtc gatggtactt tagaaaatac taacataaag gcgaaagcat 6121 cgattgtcgt ggcgaaggat aacgaggcgg aaaagggaga taaaatatca agcgccgact 6181 taaccgctgt tgtcgatccg cagttcccgc ggattattcg ttacgaggac ccgcagagta 6241 atcaggtgat cttcaatgga caacacgaga aaatcgatca ggtaatgatt gatggaaaag 6301 cgtataaggc taccgctgag aagcaaaaat ctgaagcgaa ccaagccgtc tataacgttg 6361 cggtgccaga gataggtctt cgttttacga ctacccttac cgtatcagaa ggccaagagc 6421 ttgcaatgaa actttctgac atacgggagg aagggacgaa aatacataca ataagcatac 6481 ctaaccaggg acttattagt gtcaacagca ccgatgaggg ggcgactttt gcaggcgtcg 6541 ttatgaatac tgggacgaat gcaaataatg ggaataaaaa cggcgacact atccaagacc 6601 ttaccacgac aagtcaagag gagacgaaaa aatatatgta tggattttta aacacggcga 6661 actacgcagc cagcttctgg acaaacgctt atggcgatgg aagcgtagat gggagcgaca 6721 acaatcgtat tcataagcaa accaaggaag ccgcgacggg tttcgtaacc acattatcga 6781 gcggagcctg gacctaccgt ccctttgacg caccagagga ctacacaaca ggtgaaacac 6841 cagaggtgaa ggtgaaattc agcaaggatt cgaacgacga taaccgtgtt gattggcagg 6901 acgcagcgat tgggttcaga tccatcatga acaaccccat gggcgccgaa aaggtcccag 6961 aattggtaaa tcaacgcatt ccctttaact ttgcctcgca ggcgacaaat ccgttccttg 7021 tcacgcttga tgaatcgaag cggatctata acttaacaga tggtttgggt cagatgaatt 7081 tgttgaaggg atatcagaat gagggtcacg attctgctca cccggattat ggagctatag 7141 gacagcgccc aggaggagag caagcattga atcaacttat tgacgagggt cacaagctga 7201 acgctgtgtt cggcgtccac atcaatgaca ctgagtctta ccccgaagcg aaggggttca 7261 atgaagagct ggtcgatcct actaaacgcg gttgggattg gttggacccc agttatttta 7321 ttaagcagcg ccctgacact ttgtcaggac gtcgctatga gcggttcaaa gaactgaaac 7381 agaaggcccc caatttagat tatatatatg tggatgtctg gggaaaccaa ggcgagtcgg 7441 gatgggcctc acgtcaactg agcaaagaaa tcaacagtct ggggtggttt actaccaatg 7501 aatttccgaa tgccctggag tatgattcgg tctggaatca ttggtccgcg gagaaggatt 7561 atggcggcac tactactaag ggctttaata gtacaattgt ccggtttatc cgtaatcatc 7621 agaaagacac atggataatc tcagacaatc cattattggg gggtgctgag tttgaagcgt 7681 acgaaggttg ggttggtaaa acgaatttta atacataccg gcagaagaca ttcgctataa 7741 atgttccaac gaagtttctt cagcactacc aaattacgaa ctgggagacc acaaccgcgg 7801 cggacggtca aatatatggg acaataaaat tagctaatgg agcggagaag gttacggtta 7861 cacaggcaga tgcgaattca ccgcgttcta taaccttgaa tgaaactgag gttttgaaag 7921 gcgatgcgta tttacttcca tggaacgtta atggtcagga taaattatat cactggaacc 7981 ccaagggtgg cacatcaacc tggtctttgg acaaaaaaat gcaaggtaaa acgaatctgc 8041 atctgtatga actgactgac caaggcagaa ttgataaagg tgcgatagca acaaccaata 8101 atcaagttac gatccaagct gaggcgaaca ccccatacgt aatcgcggaa ccggatagta 8161 tcgaaccaat gacgttcggt acggggactc cctttaagga tccaggattt aatgaggcaa 8221 acacgttaaa aaacaactgg aaagtattcc gcggtgatgg cgaagttaaa aaggatgcta 8281 acggggatta cgttttttcc tccgaaaagg agcgcacaga aataaagcag gatataaatc 8341 tgccaaaacc cggcaagtat tcactttacc ttaataccga gacacacgac cgcaaagcga 8401 cggtcaccgt taaaattgga gggaagaagt atactagaac cgtcaataac tctgtggcac 8461 agaattatat acaggcagat atcaaccaca cgagtcgtaa aaatccacaa tatatgcaga 8521 atatgagaat agattttgag attcctgata atgccaaaaa aggatcagtg acactggccg 8581 tagataaagg aaactcagtg acgaagttcg acgacttgcg tatagtagag cgccagacgg 8641 acattatgaa tcccgataaa cagactgtaa ttaaacagga ttttgaagat acacaagcag 8701 tagggttata tccctttgtc aaaggcagtg ctggtggtgt tgaggacccc cggatacatt 8761 tgtctgaaag aaacgaacca tacactcaat acggctggaa tggtaacttg gtaagtgatg 8821 tattagaggg taattggagc ttgaaagctc acaaacaagg agcaggactt atgttacaaa 8881 ctataccaca aaacatcaag ttcgagccga acaagaaata tacagtccag tttgactacc 8941 agacggatgg ggaaaacgta ttcaccgcgg ggaccatcaa tggggaattg aaaaataaca 9001 acgattttaa acccgtcggt gaactgacat ctactgctgc tgatgggcag acgaagcatt 9061 acgaggcgga aattataggc gatgcctctg ggaatactac ctttgggata tttacgacag 9121 gcgccgacaa ggattttatt atggacaact tcacggtcac agttgagagc aagaagctcg 9181 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt 9241 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct 9301 tgaggggttt tttgctgaaa ggaggaacta tatccggat //