LOCUS CS16-189-01-pETh 7540 bp DNA circular 27-DEC-2016 SOURCE ORGANISM COMMENT http://www.informaxinc.com http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11564125| COMMENT VNTDBDATE|-11564126| COMMENT LSOWNER| COMMENT VNTNAME|CS16-189-01-pEThis-SMT-β1-3 Galactosidase| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 599..1456 /vntifkey="4" /label=Ap CDS complement(3651..4607) /vntifkey="4" /label=lacI misc_feature 5125..5141 /vntifkey="21" /label=T7P misc_feature 7469..7515 /vntifkey="21" /label=T7T CDS 5249..5541 /vntifkey="4" /label=SMT3 sig_peptide 5228..5245 /vntifkey="94" /label=6*His misc_feature 7393..7398 /vntifkey="21" /label=BseRI misc_feature 5350..5350 /vntifkey="21" /label=Bgl-SDM exon 5098..5105 /vntifkey="61" /label=bgl-blunt misc_feature 5542..7383 /vntifkey="21" /label=KpnI misc_feature 5177..373 /vntifkey="21" /label=T7으로\sq\확인 misc_feature 5543..7378 /vntifkey="21" /label=β1-3\Galactosidase misc_feature 5528..7393 /vntifkey="21" /label=합성 BASE COUNT 1786 a 1964 c 1998 g 1792 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata 5161 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgagagg 5221 atcgcatcac catcaccatc accatggaat gtcggactca gaagtcaatc aagaagctaa 5281 gccagaggtc aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg 5341 atcttcagaa atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc 5401 gttcgctaaa agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag 5461 aattcaagct gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc 5521 tcacagagaa cagattggtg ggatgttacg gacgacgtta gcccctttag ttctggcact 5581 tgcactggct ctgccagcgg ctgctgcgac tccggagtcg tggccaactt tcggcactca 5641 agggacccag ttcgtcagag atggaaaacc ttaccagttg ttgtccggcg ctatccactt 5701 tcagcgcatc ccccgcgcat actggaagga ccgccttcaa aaggcgcgtg ctttaggatt 5761 gaatacagtc gaaacttatg tattttggaa tcttgtggag cctcagcagg gaccgtttga 5821 tttctcgggt aataacgatg tcgccgcatt cgtgaaggag gctgctgctc aaggtttaaa 5881 tgtgatatta cgtcctgggc cgtatgcatg tgccgagtgg gaagcaggcg gctatccagc 5941 ctggttattc ggcaagggta acatacgcgt gcggtcaaga gacccccgtt tcttggcagc 6001 tagccaggca tacttagacg cacttgctaa acaagtccaa cccttgctga accacaatgg 6061 tggtcctatc atagctgttc aagtcgaaaa tgagtatggc tcctatgcag atgatcatgc 6121 atacatggct gataacagag ctatgtacgt aaaagcgggc ttcgacaagg cgttattgtt 6181 tacgagtgat ggagcggata tgcttgcgaa cgggactttg ccggatacct tagcggttgt 6241 aaatttcgct ccaggggaag ctaaatctgc gtttgacaaa ttgatcaaat tccgtcctga 6301 ccaaccgcgc atggtaggag agtattgggc gggctggttt gatcactggg gaaagcccca 6361 cgcagctaca gatgcacgtc agcaggcgga ggaatttgaa tggattctgc ggcaaggaca 6421 ttctgcgaac ctttatatgt ttattggggg aacgtctttt gggtttatga acggtgctaa 6481 ctttcaaaac aaccctagcg accactacgc tccgcaaacc acttcttacg attacgacgc 6541 tatactggat gaggcaggac accctacccc taaatttgct ctgatgcgcg acgcaattgc 6601 acgggtcacc ggggtccaac ctcccgcgtt gcccgctcct ataacgacca ctacgcttcc 6661 cgccacaccc cttcgggagt ccgctagttt gtgggacaac cttcctaccc cgattgccat 6721 tgataccccc cagcctatgg aacagtttgg tcaagattat ggctacattt tgtatcgcac 6781 gaccatcacc gggccgagaa aagggccact ttacttaggc gacgtgcgcg atgtcgcccg 6841 tgtctacgta gatcagcggc cagttgggag cgtggagaga cgcctgcagc aagtttctct 6901 tgaggttgaa atcccggccg gacaacatac tcttgatgtt ctggtggaaa actccgggcg 6961 cattaattat ggcacacgca tggctgatgg ccgcgccggc cttgtggatc ctgtgttact 7021 tgactcccaa cagttaaccg gctggcaggc gtttccgtta cccatgcgca cccccgattc 7081 cataagaggc tggaccggca aagccgtaca agggcctgcc tttcatcggg gcacgctgcg 7141 tatagggact cccacggata cgtatttaga catgcgcgca ttcgggaaag gctttgcatg 7201 ggcaaacggg gttaacctgg gacgccattg gaacattggg ccccaaacgg cactttattt 7261 ccctgcgccc ttccaacgga aaggggataa tacagtggta gtttttgacc tggacgatgt 7321 cgcgactccc tcggtaagag gactgaagca gcaggtctgg ttaactccta aagaataagt 7381 accgtctaaa ctctcctcga gtgagatccg gctgctaaca aagcccgaaa ggaagctgag 7441 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 7501 ttgaggggtt ttttgctgaa aggaggaact atatccggat //