LOCUS CS17-087-02M-pLy 7442 bp DNA circular 26-SEP-2017 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11940004| COMMENT VNTDBDATE|-11957080| COMMENT LSOWNER| COMMENT VNTNAME|CS17-087-02M-pLys-M.BsmA I| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers misc_marker complement(2081..2737) /vntifkey="22" /label=Cm rep_origin 3099..4011 /vntifkey="33" /label=p15A\ori primer 4289..4308 /vntifkey="27" /label=pLysS-S-1F misc_feature complement(213..232) /vntifkey="21" /label=pLysS-S-2R primer complement(209..229) /vntifkey="27" /label=pLys-sq2121R misc_feature 4421..7441 /vntifkey="21" /label=M.BsmA\I BASE COUNT 2004 a 1773 c 1828 g 1837 t ORIGIN 1 tcgagttaat tgaactcact aaagggagac cacagcggtt tccctttgtt cgcattggag 61 gtcaaataat gcgcaagtct tataaacaat tctataaggc tccgaggagg catatccaag 121 tgtgggaggc agccaatggg ccggatcctc tacgccggac gcatcgtggc cggcatcacc 181 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 241 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 301 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 361 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 421 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 481 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 541 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 601 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 661 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 721 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 781 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 841 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 901 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 961 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 1021 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 1081 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 1141 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 1201 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 1261 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 1321 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 1381 gtgtttcgta aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac 1441 agagagtgga accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg 1501 gcctcatttc ttattctgag ttacaacagt ccgcaccgct gtccggtagc tccttccggt 1561 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg 1621 taggacaggt gccggcagcg cccaacagtc ccccggccac ggggcctgcc accataccca 1681 cgccgaaaca agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc 1741 taccctgtgg aacacctaca tctgtattaa cgaagcgcta accgttttta tcaggctctg 1801 ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa 1861 ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt 1921 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc 1981 gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac 2041 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat 2101 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat 2161 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 2221 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 2281 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 2341 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 2401 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 2461 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga 2521 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 2581 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 2641 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 2701 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 2761 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 2821 aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg 2881 tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt 2941 attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt 3001 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac 3061 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg 3121 cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa 3181 ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac 3241 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg 3301 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag 3361 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag 3421 tggtggcgaa acccgacagg actataaaga taccaggcgt ttcccctggc ggctccctcg 3481 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt 3541 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt 3601 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag 3661 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga 3721 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga 3781 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga 3841 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa 3901 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt 3961 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg 4021 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac 4081 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca 4141 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata 4201 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga 4261 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag 4321 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg 4381 tcctgtggat ccggagctca gatctgaagg agatatacat atgaatgggg gagcgtgcgt 4441 taaggagaac accgagataa atatagacat caaaaaagct gccctttggg atacgataag 4501 aaacaaaagt cagtttcttg agacgcaaat ggacccatta gagcgcaaac gtaccggtag 4561 ctatttcact gcgctggaat taacagacgt aatgatgcaa gagcttgtgt catatatatt 4621 aaaaagtgac aaggacataa cggaattgaa atttctggag ccatgcgtcg ggacgggcaa 4681 ctttgtcttt tcgtatctga aggagattag caaattgcag cttcacaagg agcaaataga 4741 aacattgatt aacaacatct acgttgcaga catcaaccaa acggcacttt tagagtataa 4801 gaagttatta tcgaaatttg ctaaacttta cttcgacatt gatctttctg aagagtattt 4861 caatagtcat attggaagtg ctcttcttat cgatgttgcg gctgaacagc cagaatatat 4921 caagatcaca gatgtgttcc ctgacgaagt agtgaaggag ggcttcgata tagttgtgac 4981 taatccgcca tataaaaacc tgaaagctga aaaggggcag tactctaacg atctggagta 5041 cgaaattgat agagcacggt atgccgaaat aaaaaaaatg gtaaagcgga tatttaatta 5101 cagtacagac ggggttttaa acctgtataa gttattcgtg gaggaaatca tagacaaata 5161 cgcaaacccc aacggttttg tatctcttct tattccgagc agcattttga ccgataagac 5221 ttgtacaaaa cttcggactc acatgttagt cgacagcaac atccttagca tcaagatgat 5281 taacgaaggc tcaggctata tagatgccca acaggccctg tcagcgatat taattcagaa 5341 aggtaaaaga accgagagca ttaaggtcac caaagattac agtaataacc ccaaccagat 5401 tacggacatt aacatggaag acatcttgaa cgagaatact ggcaacgcca tctttgcgat 5461 caataatcac gaatatttta tacttaaaca attgcgcaaa tttcctgtcg ttaaggacct 5521 tgatttcata ataaatttac ggggtgagtt ggatcttaca gctaataagg acagcatcgt 5581 gaacatcgat acgggatatc cgcttctgcg cggccggaac ataggttatt atgaaatatt 5641 ggacacatgc agtggagaat ttgtttcgaa agactttatt gaaaactcca agaaatctcg 5701 ttatattaaa gaaaagcgga tagtgtgcca acaagtcgtt aacatgaaaa aagagcgtcg 5761 tgttacgttc gcgcttgtgg aagaaaatta tgtcctgggg aactcatgta atttcataag 5821 cgtcatggac aatgactata acatcgactt gtatgcaata ctgggtttat ttaacacttc 5881 tattatcaat tggctgttca agcttacatc ttccaacaac catgtcaata attacgagat 5941 tgactgcttc ccggttccta ttgggagtcc ctatttgaat aagatatcga acttagtaaa 6001 aaaatatctg tcaaataagg actcgtcact gctggagaag atcgaggaat atgcatatat 6061 agcctacggt atccgtgaag cgaaggaaga caatgaagac aaggacgata tagcaaattt 6121 aaaggaaaca aacgacatca ttaagaagta ttacagtgca ataaagcacg ttcttccgtc 6181 gattacgctt gaggacagcg tatcgatctt agaggggcaa tccagcatag agtcattcat 6241 attgcaatcg ggggtcgagc tggacaaata tacccgcaac attgtgttgg gaatcacgga 6301 caaatatatg aagataaaga agggcgagat attgaaccac acaacgttta aattgtctga 6361 cctggatttg gaaatgatcc gttccgtgcc tccgggcggc aactggaagg acattccgat 6421 agaaactgta aaaaaattta aacggttaat gagaataacc gaaactggcg gcagaaccac 6481 attgtacggg cggatcgact atgataagcc gagttacacg attacaacat attttaaccg 6541 tccgggcaat ggcacttatg ttcatccggt acatgaccgt gtcttgtcag tacgcgaggc 6601 tgcacgcttc cagtgtttta aggacgacta ttatttttac ggtaataaaa ctcagatgct 6661 gaaacaggta ggtaacgcgg tcccgacaat tttagcgtat caaatagcta agaaaattgt 6721 cgacaaaacc gggtgccgca aaagcatcga cttgttctgt ggggcggggg gccttacagc 6781 tggattcaag gaggcaggaa tccaaagcgt cctttgtaat gatatcgaag agagcgcctg 6841 tatcacactt aagattaaca atccagaaat taaagttttg tgtggggaca tatcacaaca 6901 tgagaccaag gagcacatcg tgaatgttgc cataaatgaa gatgttgata ttatatgcgg 6961 cggcccccct tgtcagggct ttagcatggc tggccttcgc ctgactgatg accccagaaa 7021 ccagctgttt aaagaattta tagagattgt atccagagtc aaaccaaagg tcatagtatt 7081 cgaaaatgtc gaagggatct tgagcttcca atcaggtaag gtatatcgcg cgatcttgga 7141 gatgttcagc gagattggat actttactga gggaagaacc ctgatgtcga gtgactatgc 7201 ggtgccccag aaacggaaaa gagtctttat tatttgtacg agagatgata tggatgtgaa 7261 accagctgat ttgtttccaa cccccatcac cgaggagcct gaatgccaaa tcacggcacg 7321 ggacaccatc aaagacttag agaatataca gtgcgacgag aaggcatgct atgtgaaggt 7381 agaacatgaa tctgatatct tgaaggtttt caagggcaaa atgacgtatc aggaaattta 7441 cc //