LOCUS CS17-114-01-pPIC 10555 bp DNA circular 29-JAN-2018 DEFINITION pPIC9K. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12089824| COMMENT VNTDBDATE|-12137327| COMMENT LSOWNER| COMMENT VNTNAME|CS17-114-01-pPIC9K-OriSS-SAP(opti)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 1..10555 /organism="pPIC9K" /mol_type="other DNA" /vntifkey="98" misc_feature complement(2606..2626) /vntifkey="21" /label=3_AOX1_primer CDS complement(3259..5793) /vntifkey="4" /label=ORF\frame\2 gene complement(6207..7022) /gene="KanR2" /vntifkey="60" CDS complement(6207..7022) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(7187..7206) /vntifkey="21" /label=pBRrevBam_primer CDS complement(7188..7910) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(8192..8214) /vntifkey="21" /label=pGEX_3_primer rep_origin complement(8611..9230) /vntifkey="33" /label=pBR322_origin gene complement(9385..10245) /gene="Ampicillin" /vntifkey="60" CDS complement(9385..10245) /vntifkey="4" /label=ORF\frame\2 promoter complement(10287..10315) /vntifkey="30" /label=AmpR_promoter misc_feature 944..2512 /vntifkey="21" /label=OriSS-SAP(opti) misc_feature 929..1748 /vntifkey="21" /label=A-gBlock\اص¼؛ misc_feature 1734..2527 /vntifkey="21" /label=B-gBlock\اص¼؛ BASE COUNT 2857 a 2482 c 2416 g 2800 t ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccatgagag ctttctattg 961 tctattcggt ttattactaa cgctttcact acagggtgaa gccacccgtc aaccctcaag 1021 ttatgacgga gatgagtatc acgagaggta tccaggacag tcaaatccca ttacggagga 1081 ggacaaggct tactggaaca aggatgcaca ggatgcactg gacaaacaac ttggaattaa 1141 actgcgtgaa aaacaggcaa aaaacgttat atttttctta ggagacggaa tgtccctaag 1201 tacagtgacc gctgctagga tttacaaagg tggtctgacc ggcaagtttg agcgtgaaaa 1261 aatttcctgg gaggagtttg actttgctgc cctgtctaag acctacaaca ctgataaaca 1321 ggtgacagac agtgcagcct cagccactgc ctatctaact ggtgtgaaga ccaaccaagg 1381 cgtcattggt ttggatgcaa acacggtaag aacgaactgc tcctaccagc tagatgaatc 1441 tctgtttact tatagtatag ctcattggtt ccaggaggct ggccgttcaa caggagtggt 1501 aacctcaacc agggtgactc acgccacccc cgccggaacc tacgctcatg tagccgatcg 1561 tgactgggaa aacgactctg atgtcgttca cgaccgtgaa gatcccgaaa tatgtgatga 1621 catagccgag cagttggtct ttagggagcc aggaaagaac tttaaggtta tcatgggtgg 1681 aggcaggcgt ggcttctttc ccgaagaagc tcttgacatc gaggacggta tccctggtga 1741 aagggaggac ggcaaacact taattacgga ctggctggat gacaaggctt ctcaaggcgc 1801 aaccgcttcc tatgtgtgga atcgtgacga tctgctagca gtggacatta gaaatacgga 1861 ttatctgatg ggattattct cctataccca tttggataca gtcttgacca gagatgctga 1921 aatggaccct acgttgcccg agatgacgaa agttgccatt gagatgctga cgaaagatga 1981 gaatggcttt tttctattag tcgagggcgg taggatagat cacatgcatc atgccaacca 2041 gataaggcag tccttagccg aaacgttaga tatggaagag gccgtctcta tggctctaag 2101 tatgaccgac cctgaagaaa ctatcatatt ggtgacagcc gaccacggcc atacattgac 2161 cattaccggt tacgcagata ggaataccga catcctagac tttgctggaa tatctgattt 2221 ggatgacaga agatatacga ttttagacta tggttctggt cctggctacc atatcacgga 2281 ggatggaaag aggtatgagc ccacggaaga agacctaaag gatattaact ttagatatgc 2341 atccgccgcc cctaaacatt cagttacaca tgacggaacc gacgtaggca tatgggtgaa 2401 cggtccattc gctcacttgt tcactggagt gtatgaagag aattacatcc cacacgcttt 2461 agcatatgct gcctgcgtcg gaacaggtag aacattctgt gacgagaagt gagcggccgc 2521 gaattaattc gccttagaca tgactgttcc tcagttcaag ttgggcactt acgagaagac 2581 cggtcttgct agattctaat caagaggatg tcagaatgcc atttgcctga gagatgcagg 2641 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt 2701 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagctgat gaatatcttg 2761 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc 2821 ttcagagtac agaagattaa gtgagaagtt cgtttgtgca agcttatcga taagctttaa 2881 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac 2941 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt 3001 atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac 3061 tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga 3121 gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact 3181 atcgactacg cgatcatggc gaccacaccc gtcctgtgga tctatcgaat ctaaatgtaa 3241 gttaaaatct ctaaataatt aaataagtcc cagtttctcc atacgaacct taacagcatt 3301 gcggtgagca tctagacctt caacagcagc cagatccatc actgcttggc caatatgttt 3361 cagtccctca ggagttacgt cttgtgaagt gatgaacttc tggaaggttg cagtgttaac 3421 tccgctgtat tgacgggcat atccgtacgt tggcaaagtg tggttggtac cggaggagta 3481 atctccacaa ctctctggag agtaggcacc aacaaacaca gatccagcgt gttgtacttg 3541 atcaacataa gaagaagcat tctcgatttg caggatcaag tgttcaggag cgtactgatt 3601 ggacatttcc aaagcctgct cgtaggttgc aaccgatagg gttgtagagt gtgcaataca 3661 cttgcgtaca atttcaaccc ttggcaactg cacagcttgg ttgtgaacag catcttcaat 3721 tctggcaagc tccttgtctg tcatatcgac agccaacaga atcacctggg aatcaatacc 3781 atgttcagct tgagacagaa ggtctgaggc aacgaaatct ggatcagcgt atttatcagc 3841 aataactaga acttcagaag gcccagcagg catgtcaata ctacacaggg ctgatgtgtc 3901 attttgaacc atcatcttgg cagcagtaac gaactggttt cctggaccaa atattttgtc 3961 acacttagga acagtttctg ttccgtaagc catagcagct actgcctggg cgcctcctgc 4021 tagcacgata cacttagcac caaccttgtg ggcaacgtag atgacttctg gggtaagggt 4081 accatccttc ttaggtggag atgcaaaaac aatttctttg caaccagcaa ctttggcagg 4141 aacacccagc atcagggaag tggaaggcag aattgcggtt ccaccaggaa tatagaggcc 4201 aactttctca ataggtcttg caaaacgaga gcagactaca ccagggcaag tctcaacttg 4261 caacgtctcc gttagttgag cttcatggaa tttcctgacg ttatctatag agagatcaat 4321 ggctctctta acgttatctg gcaattgcat aagttcctct gggaaaggag cttctaacac 4381 aggtgtcttc aaagcgactc catcaaactt ggcagttagt tctaaaaggg ctttgtcacc 4441 attttgacga acattgtcga caattggttt gactaattcc ataatctgtt ccgttttctg 4501 gataggacga cgaagggcat cttcaatttc ttgtgaggag gccttagaaa cgtcaatttt 4561 gcacaattca atacgacctt cagaagggac ttctttaggt ttggattctt ctttaggttg 4621 ttccttggtg tatcctggct tggcatctcc tttccttcta gtgaccttta gggacttcat 4681 atccaggttt ctctccacct cgtccaacgt cacaccgtac ttggcacatc taactaatgc 4741 aaaataaaat aagtcagcac attcccaggc tatatcttcc ttggatttag cttctgcaag 4801 ttcatcagct tcctccctaa ttttagcgtt caacaaaact tcgtcgtcaa ataaccgttt 4861 ggtataagaa ccttctggag cattgctctt acgatcccac aaggtggctt ccatggctct 4921 aagacccttt gattggccaa aacaggaagt gcgttccaag tgacagaaac caacacctgt 4981 ttgttcaacc acaaatttca agcagtctcc atcacaatcc aattcgatac ccagcaactt 5041 ttgagttgct ccagatgtag cacctttata ccacaaaccg tgacgacgag attggtagac 5101 tccagtttgt gtccttatag cctccggaat agactttttg gacgagtaca ccaggcccaa 5161 cgagtaatta gaagagtcag ccaccaaagt agtgaataga ccatcggggc ggtcagtagt 5221 caaagacgcc aacaaaattt cactgacagg gaactttttg acatcttcag aaagttcgta 5281 ttcagtagtc aattgccgag catcaataat ggggattata ccagaagcaa cagtggaagt 5341 cacatctacc aactttgcgg tctcagaaaa agcataaaca gttctactac cgccattagt 5401 gaaacttttc aaatcgccca gtggagaaga aaaaggcaca gcgatactag cattagcggg 5461 caaggatgca actttatcaa ccagggtcct atagataacc ctagcgcctg ggatcatcct 5521 ttggacaact ctttctgcca aatctaggtc caaaatcact tcattgatac cattattgta 5581 caacttgagc aagttgtcga tcagctcctc aaattggtcc tctgtaacgg atgactcaac 5641 ttgcacatta acttgaagct cagtcgattg agtgaacttg atcaggttgt gcagctggtc 5701 agcagcatag ggaaacacgg cttttcctac caaactcaag gaattatcaa actctgcaac 5761 acttgcgtat gcaggtagca agggaaatgt catacttgaa gtcggacagt gagtgtagtc 5821 ttgagaaatt ctgaagccgt atttttatta tcagtgagtc agtcatcagg agatcctcta 5881 cgccggacgc atcgtggccg acctgcaggg gggggggggg cgctgaggtc tgcctcgtga 5941 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag 6001 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 6061 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 6121 aaaagttcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag 6181 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc 6241 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 6301 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 6361 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 6421 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt 6481 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca 6541 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta 6601 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca 6661 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg 6721 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga 6781 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca 6841 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga 6901 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca 6961 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc 7021 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata 7081 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc 7141 cccctgcagg tcggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac 7201 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg 7261 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca 7321 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag 7381 gagtcgcata agggagagcg tcgagtatct atgattggaa gtatgggaat ggtgataccc 7441 gcattcttca gtgtcttgag gtctcctatc agattatgcc caactaaagc aaccggagga 7501 ggagatttca tggtaaattt ctctgacttt tggtcatcag tagactcgaa ctgtgagact 7561 atctcggtta tgacagcaga aatgtccttc ttggagacag taaatgaagt cccaccaata 7621 aagaaatcct tgttatcagg aacaaacttc ttgtttcgaa ctttttcggt gccttgaact 7681 ataaaatgta gagtggatat gtcgggtagg aatggagcgg gcaaatgctt accttctgga 7741 ccttcaagag gtatgtaggg tttgtagata ctgatgccaa cttcagtgac aacgttgcta 7801 tttcgttcaa accattccga atccagagaa atcaaagttg tttgtctact attgatccaa 7861 gccagtgcgg tcttgaaact gacaatagtg tgctcgtgtt ttgaggtcat ctttgtatga 7921 ataaatctag tctttgatct aaataatctt gacgagccaa ggcgataaat acccaaatct 7981 aaaactcttt taaaacgtta aaaggacaag tatgtctgcc tgtattaaac cccaaatcag 8041 ctcgtagtct gatcctcatc aacttgaggg gcactatctt gttttagaga aatttgcgga 8101 gatgcgatat cgagaaaaag gtacgctgat tttaaacgtg aaatttatct caagatctct 8161 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 8221 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 8281 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 8341 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 8401 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8821 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 9061 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 10381 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 10441 tcgtcttcaa gaattaattc tcatgtttga cagcttatca tcgataagct gactcatgtt 10501 ggtattgtga aatagacgca gatcgggaac actgaaaaat aacagttatt attcg //