LOCUS CS17-114-02-pPIC 10498 bp DNA circular 27-DEC-2017 DEFINITION pPIC9K. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12089830| COMMENT VNTDBDATE|-12089830| COMMENT LSOWNER| COMMENT VNTNAME|CS17-114-02-pPIC9K-SS7-duplex-SAP(opti)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 1..10498 /organism="pPIC9K" /mol_type="other DNA" /vntifkey="98" misc_feature complement(2549..2569) /vntifkey="21" /label=3_AOX1_primer CDS complement(3202..5736) /vntifkey="4" /label=ORF\frame\2 gene complement(6150..6965) /gene="KanR2" /vntifkey="60" CDS complement(6150..6965) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(7130..7149) /vntifkey="21" /label=pBRrevBam_primer CDS complement(7131..7853) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(8135..8157) /vntifkey="21" /label=pGEX_3_primer rep_origin complement(8554..9173) /vntifkey="33" /label=pBR322_origin gene complement(9328..10188) /gene="Ampicillin" /vntifkey="60" CDS complement(9328..10188) /vntifkey="4" /label=ORF\frame\2 promoter complement(10230..10258) /vntifkey="30" /label=AmpR_promoter misc_feature 944..1027 /vntifkey="21" /label=SS7 misc_feature 1028..2455 /vntifkey="21" /label=SAP(opti) BASE COUNT 2844 a 2468 c 2395 g 2791 t ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccgaaacga tgactaagcc 961 aacccaagta ttagttagat ccgtcagtat attatttttc atcacattac tacatctagt 1021 cgtagctaag gcttactgga acaaggatgc acaggatgca ctggacaaac aacttggaat 1081 taaactgcgt gaaaaacagg caaaaaacgt tatatttttc ttaggagacg gaatgtccct 1141 aagtacagtg accgctgcta ggatttacaa aggtggtctg accggcaagt ttgagcgtga 1201 aaaaatttcc tgggaggagt ttgactttgc tgccctgtct aagacctaca acactgataa 1261 acaggtgaca gacagtgcag cctcagccac tgcctatcta actggtgtga agaccaacca 1321 aggcgtcatt ggtttggatg caaacacggt aagaacgaac tgctcctacc agctagatga 1381 atctctgttt acttatagta tagctcattg gttccaggag gctggccgtt caacaggagt 1441 ggtaacctca accagggtga ctcacgccac ccccgccgga acctacgctc atgtagccga 1501 tcgtgactgg gaaaacgact ctgatgtcgt tcacgaccgt gaagatcccg aaatatgtga 1561 tgacatagcc gagcagttgg tctttaggga gccaggaaag aactttaagg ttatcatggg 1621 tggaggcagg cgtggcttct ttcccgaaga agctcttgac atcgaggacg gtatccctgg 1681 tgaaagggag gacggcaaac acttaattac ggactggctg gatgacaagg cttctcaagg 1741 cgcaaccgct tcctatgtgt ggaatcgtga cgatctgcta gcagtggaca ttagaaatac 1801 ggattatctg atgggattat tctcctatac ccatttggat acagtcttga ccagagatgc 1861 tgaaatggac cctacgttgc ccgagatgac gaaagttgcc attgagatgc tgacgaaaga 1921 tgagaatggc ttttttctat tagtcgaggg cggtaggata gatcacatgc atcatgccaa 1981 ccagataagg cagtccttag ccgaaacgtt agatatggaa gaggccgtct ctatggctct 2041 aagtatgacc gaccctgaag aaactatcat attggtgaca gccgaccacg gccatacatt 2101 gaccattacc ggttacgcag ataggaatac cgacatccta gactttgctg gaatatctga 2161 tttggatgac agaagatata cgattttaga ctatggttct ggtcctggct accatatcac 2221 ggaggatgga aagaggtatg agcccacgga agaagaccta aaggatatta actttagata 2281 tgcatccgcc gcccctaaac attcagttac acatgacgga accgacgtag gcatatgggt 2341 gaacggtcca ttcgctcact tgttcactgg agtgtatgaa gagaattaca tcccacacgc 2401 tttagcatat gctgcctgcg tcggaacagg tagaacattc tgtgacgaga agtgagcggc 2461 cgcgaattaa ttcgccttag acatgactgt tcctcagttc aagttgggca cttacgagaa 2521 gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc tgagagatgc 2581 aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt ttttttgtca 2641 ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct gatgaatatc 2701 ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta tttcccactc 2761 ctcttcagag tacagaagat taagtgagaa gttcgtttgt gcaagcttat cgataagctt 2821 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct 2881 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg 2941 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt 3001 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc 3061 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc 3121 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatctatcg aatctaaatg 3181 taagttaaaa tctctaaata attaaataag tcccagtttc tccatacgaa ccttaacagc 3241 attgcggtga gcatctagac cttcaacagc agccagatcc atcactgctt ggccaatatg 3301 tttcagtccc tcaggagtta cgtcttgtga agtgatgaac ttctggaagg ttgcagtgtt 3361 aactccgctg tattgacggg catatccgta cgttggcaaa gtgtggttgg taccggagga 3421 gtaatctcca caactctctg gagagtaggc accaacaaac acagatccag cgtgttgtac 3481 ttgatcaaca taagaagaag cattctcgat ttgcaggatc aagtgttcag gagcgtactg 3541 attggacatt tccaaagcct gctcgtaggt tgcaaccgat agggttgtag agtgtgcaat 3601 acacttgcgt acaatttcaa cccttggcaa ctgcacagct tggttgtgaa cagcatcttc 3661 aattctggca agctccttgt ctgtcatatc gacagccaac agaatcacct gggaatcaat 3721 accatgttca gcttgagaca gaaggtctga ggcaacgaaa tctggatcag cgtatttatc 3781 agcaataact agaacttcag aaggcccagc aggcatgtca atactacaca gggctgatgt 3841 gtcattttga accatcatct tggcagcagt aacgaactgg tttcctggac caaatatttt 3901 gtcacactta ggaacagttt ctgttccgta agccatagca gctactgcct gggcgcctcc 3961 tgctagcacg atacacttag caccaacctt gtgggcaacg tagatgactt ctggggtaag 4021 ggtaccatcc ttcttaggtg gagatgcaaa aacaatttct ttgcaaccag caactttggc 4081 aggaacaccc agcatcaggg aagtggaagg cagaattgcg gttccaccag gaatatagag 4141 gccaactttc tcaataggtc ttgcaaaacg agagcagact acaccagggc aagtctcaac 4201 ttgcaacgtc tccgttagtt gagcttcatg gaatttcctg acgttatcta tagagagatc 4261 aatggctctc ttaacgttat ctggcaattg cataagttcc tctgggaaag gagcttctaa 4321 cacaggtgtc ttcaaagcga ctccatcaaa cttggcagtt agttctaaaa gggctttgtc 4381 accattttga cgaacattgt cgacaattgg tttgactaat tccataatct gttccgtttt 4441 ctggatagga cgacgaaggg catcttcaat ttcttgtgag gaggccttag aaacgtcaat 4501 tttgcacaat tcaatacgac cttcagaagg gacttcttta ggtttggatt cttctttagg 4561 ttgttccttg gtgtatcctg gcttggcatc tcctttcctt ctagtgacct ttagggactt 4621 catatccagg tttctctcca cctcgtccaa cgtcacaccg tacttggcac atctaactaa 4681 tgcaaaataa aataagtcag cacattccca ggctatatct tccttggatt tagcttctgc 4741 aagttcatca gcttcctccc taattttagc gttcaacaaa acttcgtcgt caaataaccg 4801 tttggtataa gaaccttctg gagcattgct cttacgatcc cacaaggtgg cttccatggc 4861 tctaagaccc tttgattggc caaaacagga agtgcgttcc aagtgacaga aaccaacacc 4921 tgtttgttca accacaaatt tcaagcagtc tccatcacaa tccaattcga tacccagcaa 4981 cttttgagtt gctccagatg tagcaccttt ataccacaaa ccgtgacgac gagattggta 5041 gactccagtt tgtgtcctta tagcctccgg aatagacttt ttggacgagt acaccaggcc 5101 caacgagtaa ttagaagagt cagccaccaa agtagtgaat agaccatcgg ggcggtcagt 5161 agtcaaagac gccaacaaaa tttcactgac agggaacttt ttgacatctt cagaaagttc 5221 gtattcagta gtcaattgcc gagcatcaat aatggggatt ataccagaag caacagtgga 5281 agtcacatct accaactttg cggtctcaga aaaagcataa acagttctac taccgccatt 5341 agtgaaactt ttcaaatcgc ccagtggaga agaaaaaggc acagcgatac tagcattagc 5401 gggcaaggat gcaactttat caaccagggt cctatagata accctagcgc ctgggatcat 5461 cctttggaca actctttctg ccaaatctag gtccaaaatc acttcattga taccattatt 5521 gtacaacttg agcaagttgt cgatcagctc ctcaaattgg tcctctgtaa cggatgactc 5581 aacttgcaca ttaacttgaa gctcagtcga ttgagtgaac ttgatcaggt tgtgcagctg 5641 gtcagcagca tagggaaaca cggcttttcc taccaaactc aaggaattat caaactctgc 5701 aacacttgcg tatgcaggta gcaagggaaa tgtcatactt gaagtcggac agtgagtgta 5761 gtcttgagaa attctgaagc cgtattttta ttatcagtga gtcagtcatc aggagatcct 5821 ctacgccgga cgcatcgtgg ccgacctgca gggggggggg gggcgctgag gtctgcctcg 5881 tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt 5941 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt 6001 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc 6061 agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc 6121 cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac 6181 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat 6241 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg 6301 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta 6361 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc 6421 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca 6481 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg 6541 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca 6601 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg 6661 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc 6721 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg 6781 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat 6841 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa 6901 tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg 6961 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat 7021 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttccccc 7081 ccccccctgc aggtcggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 7141 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 7201 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 7261 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 7321 caggagtcgc ataagggaga gcgtcgagta tctatgattg gaagtatggg aatggtgata 7381 cccgcattct tcagtgtctt gaggtctcct atcagattat gcccaactaa agcaaccgga 7441 ggaggagatt tcatggtaaa tttctctgac ttttggtcat cagtagactc gaactgtgag 7501 actatctcgg ttatgacagc agaaatgtcc ttcttggaga cagtaaatga agtcccacca 7561 ataaagaaat ccttgttatc aggaacaaac ttcttgtttc gaactttttc ggtgccttga 7621 actataaaat gtagagtgga tatgtcgggt aggaatggag cgggcaaatg cttaccttct 7681 ggaccttcaa gaggtatgta gggtttgtag atactgatgc caacttcagt gacaacgttg 7741 ctatttcgtt caaaccattc cgaatccaga gaaatcaaag ttgtttgtct actattgatc 7801 caagccagtg cggtcttgaa actgacaata gtgtgctcgt gttttgaggt catctttgta 7861 tgaataaatc tagtctttga tctaaataat cttgacgagc caaggcgata aatacccaaa 7921 tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct gcctgtatta aaccccaaat 7981 cagctcgtag tctgatcctc atcaacttga ggggcactat cttgttttag agaaatttgc 8041 ggagatgcga tatcgagaaa aaggtacgct gattttaaac gtgaaattta tctcaagatc 8101 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 8161 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 8221 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 8281 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 8341 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 8401 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8461 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8521 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8581 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8641 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8701 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8761 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8821 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8881 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8941 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9001 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9061 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9181 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9241 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 9661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 9781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 9841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc 9961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 10021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 10081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 10141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 10321 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 10381 ctttcgtctt caagaattaa ttctcatgtt tgacagctta tcatcgataa gctgactcat 10441 gttggtattg tgaaatagac gcagatcggg aacactgaaa aataacagtt attattcg //