LOCUS CS17-114-04-pPIC 10516 bp DNA circular 27-DEC-2017 DEFINITION pPIC9K. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12089834| COMMENT VNTDBDATE|-12089834| COMMENT LSOWNER| COMMENT VNTNAME|CS17-114-04-pPIC9K-SS7-duplex-SAP(opti)-6XHIS| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 1..10516 /organism="pPIC9K" /mol_type="other DNA" /vntifkey="98" misc_feature complement(2567..2587) /vntifkey="21" /label=3_AOX1_primer CDS complement(3220..5754) /vntifkey="4" /label=ORF\frame\2 gene complement(6168..6983) /gene="KanR2" /vntifkey="60" CDS complement(6168..6983) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(7148..7167) /vntifkey="21" /label=pBRrevBam_primer CDS complement(7149..7871) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(8153..8175) /vntifkey="21" /label=pGEX_3_primer rep_origin complement(8572..9191) /vntifkey="33" /label=pBR322_origin gene complement(9346..10206) /gene="Ampicillin" /vntifkey="60" CDS complement(9346..10206) /vntifkey="4" /label=ORF\frame\2 promoter complement(10248..10276) /vntifkey="30" /label=AmpR_promoter misc_feature 944..1027 /vntifkey="21" /label=SS7 misc_feature 1028..2473 /vntifkey="21" /label=SAP(opti) misc_feature 2453..2470 /vntifkey="21" /label=6XHIS BASE COUNT 2850 a 2480 c 2395 g 2791 t ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccgaaacga tgactaagcc 961 aacccaagta ttagttagat ccgtcagtat attatttttc atcacattac tacatctagt 1021 cgtagctaag gcttactgga acaaggatgc acaggatgca ctggacaaac aacttggaat 1081 taaactgcgt gaaaaacagg caaaaaacgt tatatttttc ttaggagacg gaatgtccct 1141 aagtacagtg accgctgcta ggatttacaa aggtggtctg accggcaagt ttgagcgtga 1201 aaaaatttcc tgggaggagt ttgactttgc tgccctgtct aagacctaca acactgataa 1261 acaggtgaca gacagtgcag cctcagccac tgcctatcta actggtgtga agaccaacca 1321 aggcgtcatt ggtttggatg caaacacggt aagaacgaac tgctcctacc agctagatga 1381 atctctgttt acttatagta tagctcattg gttccaggag gctggccgtt caacaggagt 1441 ggtaacctca accagggtga ctcacgccac ccccgccgga acctacgctc atgtagccga 1501 tcgtgactgg gaaaacgact ctgatgtcgt tcacgaccgt gaagatcccg aaatatgtga 1561 tgacatagcc gagcagttgg tctttaggga gccaggaaag aactttaagg ttatcatggg 1621 tggaggcagg cgtggcttct ttcccgaaga agctcttgac atcgaggacg gtatccctgg 1681 tgaaagggag gacggcaaac acttaattac ggactggctg gatgacaagg cttctcaagg 1741 cgcaaccgct tcctatgtgt ggaatcgtga cgatctgcta gcagtggaca ttagaaatac 1801 ggattatctg atgggattat tctcctatac ccatttggat acagtcttga ccagagatgc 1861 tgaaatggac cctacgttgc ccgagatgac gaaagttgcc attgagatgc tgacgaaaga 1921 tgagaatggc ttttttctat tagtcgaggg cggtaggata gatcacatgc atcatgccaa 1981 ccagataagg cagtccttag ccgaaacgtt agatatggaa gaggccgtct ctatggctct 2041 aagtatgacc gaccctgaag aaactatcat attggtgaca gccgaccacg gccatacatt 2101 gaccattacc ggttacgcag ataggaatac cgacatccta gactttgctg gaatatctga 2161 tttggatgac agaagatata cgattttaga ctatggttct ggtcctggct accatatcac 2221 ggaggatgga aagaggtatg agcccacgga agaagaccta aaggatatta actttagata 2281 tgcatccgcc gcccctaaac attcagttac acatgacgga accgacgtag gcatatgggt 2341 gaacggtcca ttcgctcact tgttcactgg agtgtatgaa gagaattaca tcccacacgc 2401 tttagcatat gctgcctgcg tcggaacagg tagaacattc tgtgacgaga agcaccacca 2461 ccaccaccac tgagcggccg cgaattaatt cgccttagac atgactgttc ctcagttcaa 2521 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc 2581 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt 2641 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc 2701 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt 2761 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagaagt tcgtttgtgc 2821 aagcttatcg ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag 2881 gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat 2941 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat 3001 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt 3061 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc 3121 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg 3181 atctatcgaa tctaaatgta agttaaaatc tctaaataat taaataagtc ccagtttctc 3241 catacgaacc ttaacagcat tgcggtgagc atctagacct tcaacagcag ccagatccat 3301 cactgcttgg ccaatatgtt tcagtccctc aggagttacg tcttgtgaag tgatgaactt 3361 ctggaaggtt gcagtgttaa ctccgctgta ttgacgggca tatccgtacg ttggcaaagt 3421 gtggttggta ccggaggagt aatctccaca actctctgga gagtaggcac caacaaacac 3481 agatccagcg tgttgtactt gatcaacata agaagaagca ttctcgattt gcaggatcaa 3541 gtgttcagga gcgtactgat tggacatttc caaagcctgc tcgtaggttg caaccgatag 3601 ggttgtagag tgtgcaatac acttgcgtac aatttcaacc cttggcaact gcacagcttg 3661 gttgtgaaca gcatcttcaa ttctggcaag ctccttgtct gtcatatcga cagccaacag 3721 aatcacctgg gaatcaatac catgttcagc ttgagacaga aggtctgagg caacgaaatc 3781 tggatcagcg tatttatcag caataactag aacttcagaa ggcccagcag gcatgtcaat 3841 actacacagg gctgatgtgt cattttgaac catcatcttg gcagcagtaa cgaactggtt 3901 tcctggacca aatattttgt cacacttagg aacagtttct gttccgtaag ccatagcagc 3961 tactgcctgg gcgcctcctg ctagcacgat acacttagca ccaaccttgt gggcaacgta 4021 gatgacttct ggggtaaggg taccatcctt cttaggtgga gatgcaaaaa caatttcttt 4081 gcaaccagca actttggcag gaacacccag catcagggaa gtggaaggca gaattgcggt 4141 tccaccagga atatagaggc caactttctc aataggtctt gcaaaacgag agcagactac 4201 accagggcaa gtctcaactt gcaacgtctc cgttagttga gcttcatgga atttcctgac 4261 gttatctata gagagatcaa tggctctctt aacgttatct ggcaattgca taagttcctc 4321 tgggaaagga gcttctaaca caggtgtctt caaagcgact ccatcaaact tggcagttag 4381 ttctaaaagg gctttgtcac cattttgacg aacattgtcg acaattggtt tgactaattc 4441 cataatctgt tccgttttct ggataggacg acgaagggca tcttcaattt cttgtgagga 4501 ggccttagaa acgtcaattt tgcacaattc aatacgacct tcagaaggga cttctttagg 4561 tttggattct tctttaggtt gttccttggt gtatcctggc ttggcatctc ctttccttct 4621 agtgaccttt agggacttca tatccaggtt tctctccacc tcgtccaacg tcacaccgta 4681 cttggcacat ctaactaatg caaaataaaa taagtcagca cattcccagg ctatatcttc 4741 cttggattta gcttctgcaa gttcatcagc ttcctcccta attttagcgt tcaacaaaac 4801 ttcgtcgtca aataaccgtt tggtataaga accttctgga gcattgctct tacgatccca 4861 caaggtggct tccatggctc taagaccctt tgattggcca aaacaggaag tgcgttccaa 4921 gtgacagaaa ccaacacctg tttgttcaac cacaaatttc aagcagtctc catcacaatc 4981 caattcgata cccagcaact tttgagttgc tccagatgta gcacctttat accacaaacc 5041 gtgacgacga gattggtaga ctccagtttg tgtccttata gcctccggaa tagacttttt 5101 ggacgagtac accaggccca acgagtaatt agaagagtca gccaccaaag tagtgaatag 5161 accatcgggg cggtcagtag tcaaagacgc caacaaaatt tcactgacag ggaacttttt 5221 gacatcttca gaaagttcgt attcagtagt caattgccga gcatcaataa tggggattat 5281 accagaagca acagtggaag tcacatctac caactttgcg gtctcagaaa aagcataaac 5341 agttctacta ccgccattag tgaaactttt caaatcgccc agtggagaag aaaaaggcac 5401 agcgatacta gcattagcgg gcaaggatgc aactttatca accagggtcc tatagataac 5461 cctagcgcct gggatcatcc tttggacaac tctttctgcc aaatctaggt ccaaaatcac 5521 ttcattgata ccattattgt acaacttgag caagttgtcg atcagctcct caaattggtc 5581 ctctgtaacg gatgactcaa cttgcacatt aacttgaagc tcagtcgatt gagtgaactt 5641 gatcaggttg tgcagctggt cagcagcata gggaaacacg gcttttccta ccaaactcaa 5701 ggaattatca aactctgcaa cacttgcgta tgcaggtagc aagggaaatg tcatacttga 5761 agtcggacag tgagtgtagt cttgagaaat tctgaagccg tatttttatt atcagtgagt 5821 cagtcatcag gagatcctct acgccggacg catcgtggcc gacctgcagg gggggggggg 5881 gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc 5941 atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca 6001 gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt 6061 gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa 6121 gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca 6181 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga 6241 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga 6301 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc 6361 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag 6421 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg 6481 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga 6541 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc 6601 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc 6661 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg 6721 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc 6781 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca 6841 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc 6901 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac 6961 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt 7021 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac 7081 acaacgtggc tttccccccc ccccctgcag gtcggcatca ccggcgccac aggtgcggtt 7141 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 7201 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 7261 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta 7321 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagtatc tatgattgga 7381 agtatgggaa tggtgatacc cgcattcttc agtgtcttga ggtctcctat cagattatgc 7441 ccaactaaag caaccggagg aggagatttc atggtaaatt tctctgactt ttggtcatca 7501 gtagactcga actgtgagac tatctcggtt atgacagcag aaatgtcctt cttggagaca 7561 gtaaatgaag tcccaccaat aaagaaatcc ttgttatcag gaacaaactt cttgtttcga 7621 actttttcgg tgccttgaac tataaaatgt agagtggata tgtcgggtag gaatggagcg 7681 ggcaaatgct taccttctgg accttcaaga ggtatgtagg gtttgtagat actgatgcca 7741 acttcagtga caacgttgct atttcgttca aaccattccg aatccagaga aatcaaagtt 7801 gtttgtctac tattgatcca agccagtgcg gtcttgaaac tgacaatagt gtgctcgtgt 7861 tttgaggtca tctttgtatg aataaatcta gtctttgatc taaataatct tgacgagcca 7921 aggcgataaa tacccaaatc taaaactctt ttaaaacgtt aaaaggacaa gtatgtctgc 7981 ctgtattaaa ccccaaatca gctcgtagtc tgatcctcat caacttgagg ggcactatct 8041 tgttttagag aaatttgcgg agatgcgata tcgagaaaaa ggtacgctga ttttaaacgt 8101 gaaatttatc tcaagatctc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 8161 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8221 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 8281 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 8341 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 8401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8761 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 9001 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 9061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 9121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 9181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 9241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 9301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9661 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9961 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 10021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 10081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 10141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 10201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 10261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 10321 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10381 taggcgtatc acgaggccct ttcgtcttca agaattaatt ctcatgtttg acagcttatc 10441 atcgataagc tgactcatgt tggtattgtg aaatagacgc agatcgggaa cactgaaaaa 10501 taacagttat tattcg //