LOCUS Exported 5438 bp DNA circular SYN 19-MAR-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . SOURCE synthetic DNA construct. ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5438) AUTHORS . TITLE Direct Submission COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT LSOWNER| COMMENT VNTNAME|Exported| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 189..211 /vntifkey="32" /label=RBS_1 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 219..251 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /vntifkey="4" /label=T7\tag\(gene\10\leader) /note="promotes efficient translation in E. coli" CDS 300..317 /codon_start=1 /product="6xHis affinity tag" /vntifkey="4" /label=6xHis terminator 384..431 /vntifkey="43" /label=T7\terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 468..923 /direction=RIGHT /vntifkey="33" /label=f1\ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 949..1053 /gene="bla" /vntifkey="30" /label=AmpR\promoter CDS 1054..1914 /codon_start=1 /gene="bla" /product="beta-lactamase" /vntifkey="4" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 2085..2673 /direction=RIGHT /vntifkey="33" /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 2859..2998 /vntifkey="21" /label=bom /note="basis of mobility region from pBR322" CDS complement(3100..3291) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /vntifkey="4" /label=rop CDS complement(4100..5182) /codon_start=1 /gene="lacI" /product="lac repressor" /vntifkey="4" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5183..5260) /gene="lacI" /vntifkey="30" /label=lacI\promoter BASE COUNT 1267 a 1440 c 1446 g 1285 t ORIGIN 1 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc 61 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat 121 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta 181 gaaataattt tgtttaactt taagaaggag atatacatat ggctagcatg actggtggac 241 agcaaatggg tcgggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc 301 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg 361 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga 421 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag 481 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 541 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 601 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 661 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 721 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 781 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 841 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 901 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 961 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1021 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 1081 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 1141 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 1201 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 1261 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 1321 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 1381 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 1441 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 1501 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 1561 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc 1621 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 1681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 1741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 1801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 1861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 1921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 1981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 2041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 2221 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 2281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 2341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2701 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 2761 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 2821 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 2881 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg 2941 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc 3001 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 3061 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 3121 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca 3181 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg 3241 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc 3301 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct 3361 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca 3421 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt 3481 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg 3541 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc 3601 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt 3661 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg 3721 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat 3781 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc 3841 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg 3901 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa 3961 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac 4021 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact 4081 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 4141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg 4201 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag 4261 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg 4321 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga 4381 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct 4441 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt 4501 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat 4561 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg 4621 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc 4681 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa 4741 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca 4801 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt 4861 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat 4921 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg 4981 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa 5041 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc 5101 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat 5161 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc 5221 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc 5281 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 5341 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 5401 gccaccatac ccacgccgaa acaagcgctc atgagccc //