LOCUS RC2205-07\\pET-2 6093 bp DNA circular 29-MAY-2024 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-15466357| COMMENT VNTDBDATE|-15466359| COMMENT LSOWNER| COMMENT VNTNAME|RC2205-07 pET-28 rc+smt3-SC-PPpase(native)_240529| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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1) COMMENT #158=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #120# -375390495 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 28 10 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.1976 0.611111 128 COMMENT "{\\i Xho}I (5932)" "@N (@S)" 65535 -6.60828 COMMENT 11.976 0 -10 3.12821 0.641026 #157#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #159=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -986 100) COMMENT (CObjectList) (CObjectList)) COMMENT #160=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -384684216 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList #161=(XLATFRAGMENT 5066 342 0 1 5067 1026)) COMMENT (CObList #162=(AnlzFontColorItem 5066 5500 37173247) COMMENT #163=(AnlzXLATItem 5066 6092 3 5066 6092)) 0 COMMENT (CObList #164=(AnlzFontBackColorItem 5066 6092 40304639))) (CObList) 8 COMMENT (CStringList) 142 124 (CObList))) FEATURES Location/Qualifiers CDS 5043..5059 /vntifkey="4" /label=T7\transl\en\RBS CDS 4980..4998 /vntifkey="4" /label=T7\promoter CDS 4998..5025 /vntifkey="4" /label=lacO CDS complement(3511..4602) /vntifkey="4" /label=lacI CDS complement(2511..2702) /vntifkey="4" /label=ROP CDS 2495..2517 /vntifkey="4" /label=pGEX_3_primer CDS 4877..4896 /vntifkey="4" /label=pBRrevBam_primer 3'clip complement(559..1374) /vntifkey="49" /label=Kan CDS 1480..2099 /vntifkey="4" /label=pBR322\origin misc_feature 5067..6093 /vntifkey="21" /label=SC-PPpase BASE COUNT 1508 a 1596 c 1525 g 1464 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca 841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca 961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt 1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt 1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc 1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac 1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat 2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc 2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga 2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc 2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc 2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga 2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt 2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa 2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg 2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa 3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag 3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc 3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg 3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct 3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc 3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt 3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg 3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga 3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc 3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc 3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc 3781 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc 3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat 3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg 3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac 4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc 4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc 4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc 4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg 4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc 4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc 4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac 4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc 4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata 4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc 4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc 4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt 4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg 4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag 4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc 4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt 4981 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt 5041 gtttaacttt aagaaggaga tataccatga cctacactac cagacaaatt ggtgccaaga 5101 acaccttgga atacaaagtt tatatcgaga aggatggtaa gccagtttct gccttccacg 5161 acattccctt gtacgctgac aaggaaaaca acattttcaa catggttgtt gaaattccac 5221 gttggaccaa cgccaagtta gaaatcacca aggaagaaac tttgaaccca atcatccaag 5281 acaccaagaa gggcaagttg agatttgtta gaaactgttt ccctcaccat ggttacattc 5341 acaactatgg tgctttccca caaacttggg aagacccaaa cgtaagccac ccagaaacta 5401 aggcagttgg tgacaacgat ccaattgatg tgttggaaat tggtgaaact attgcttaca 5461 ctggtcaagt caagcaggtt aaggctctag gtatcatggc tttattggat gaaggtgaga 5521 ccgattggaa agttattgcc attgatatta acgatccatt agccccaaaa ttgaacgaca 5581 ttgaggatgt tgagaaatac ttcccaggtc tattgagggc tactaacgaa tggttcagaa 5641 tttacaaaat cccagatggt aagccagaaa accaatttgc cttctccggt gaagctaaga 5701 acaagaagta cgctttggat atcatcaagg aaacacatga ctcctggaaa caattaattg 5761 ctggtaagtc ttctgacagc aagggtattg atttgaccaa tgttactttg cctgacaccc 5821 caacctactc caaggctgcc tctgatgcca tcccaccagc ttctccaaag gcagatgctc 5881 caattgacaa gtctattgac aagtggttct tcatctccgg ttctgtttaa ctcgagcacc 5941 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg 6001 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg 6061 gttttttgct gaaaggagga actatatccg gat //