LOCUS pcDNA3 5445 bp DNA circular SYN 19-MAR-1980
DEFINITION Mammalian expression vector with the CMV promoter and a neomycin-resistance marker.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.
SOURCE synthetic DNA construct.
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5445)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5445)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 5445)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
SGRef: number: 2; type: "Journal Article"
The cloned gene must include a Kozak sequence and start codon.
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
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FEATURES Location/Qualifiers
source 1..5445
/mol_type="other DNA"
/organism="synthetic DNA construct"
/vntifkey="98"
enhancer 235..614
/vntifkey="9"
/label=CMV\enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/vntifkey="30"
/label=CMV\promoter
/note="human cytomegalovirus (CMV) immediate early promoter"
promoter 863..881
/vntifkey="30"
/label=T7\promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 889..994
/vntifkey="21"
/label=MCS
/note="MCS multiple cloning site"
promoter complement(998..1016)
/vntifkey="30"
/label=SP6\promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 1042..1266
/vntifkey="25"
/label=bGH\poly(A)\signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1312..1740
/vntifkey="33"
/label=f1\ori
/note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
promoter 1754..2083
/vntifkey="30"
/label=SV40\promoter
/note="SV40 enhancer and early promoter"
CDS 2150..2941
/vntifkey="4"
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3118..3251
/vntifkey="25"
/label=SV40\poly(A)\signal
/note="SV40 polyadenylation signal"
primer_bind complement(3288..3304)
/vntifkey="28"
/label=M13\rev
/note="common sequencing primer, one of multiple similar variants"
protein_bind complement(3312..3328)
/vntifkey="31"
/label=lac\operator
/note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3336..3366)
/vntifkey="30"
/label=lac\promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3381..3402)
/vntifkey="31"
/label=CAP\binding\site
/note="CAP binding activates transcription in the presence of cAMP."
rep_origin complement(3690..4278)
/direction=LEFT
/vntifkey="33"
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
CDS complement(4452..5309)
/vntifkey="4"
/label=AmpR
/note="beta-lactamase"
promoter complement(5310..5414)
/vntifkey="30"
/label=AmpR\promoter
BASE COUNT 1255 a 1418 c 1388 g 1384 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
961 ctggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
1021 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
1081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
1141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
1201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
1261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
1321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
1381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
1441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
1501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
1561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
1621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
1681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
1741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
1801 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
1861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
1921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
1981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
2041 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
2101 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
2161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
2221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
2281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
2341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
2401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
2461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
2521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
2581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
2641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
2701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
2761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
2821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
2881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
2941 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
3001 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
3061 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
3121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
3241 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
3301 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
3361 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
3421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
3661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
3721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
3781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
3841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
3901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
3961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
4021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
4081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
4141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
4201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
4261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
4321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
4381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
4441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
4501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
4561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
4621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
4681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
4741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
4801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
4861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
4921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
4981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
5041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
5101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
5161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
5221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
5281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
5341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
5401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//