LOCUS Exported 5014 bp DNA circular SYN 19-MAR-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pcDNA3; 1/Zeo(+). SOURCE synthetic DNA construct. ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5014) AUTHORS 11111111 TITLE Direct Submission JOURNAL Exported Sep 10, 2025 from SnapGene 6.0.2 https://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT LSOWNER| COMMENT VNTNAME|Exported| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/vntifkey="30" /label=CMV\promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /vntifkey="30" /label=T7\promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1028..1252 /vntifkey="25" /label=bGH\poly(A)\signal /note="bovine growth hormone polyadenylation signal" rep_origin 1298..1726 /direction=RIGHT /vntifkey="33" /label=f1\ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1740..2069 /vntifkey="30" /label=SV40\promoter /note="SV40 enhancer and early promoter" rep_origin 1920..2055 /vntifkey="33" /label=SV40\ori /note="SV40 origin of replication" promoter 2117..2164 /vntifkey="30" /label=EM7\promoter /note="synthetic bacterial promoter" CDS 2183..2557 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /vntifkey="4" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" polyA_signal 2687..2808 /vntifkey="25" /label=SV40\poly(A)\signal /note="SV40 polyadenylation signal" primer_bind complement(2857..2873) /vntifkey="28" /label=M13\rev /note="common sequencing primer, one of multiple similar variants" protein_bind 2881..2897 /bound_moiety="lac repressor encoded by lacI" /vntifkey="31" /label=lac\operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2905..2935) /vntifkey="30" /label=lac\promoter /note="promoter for the E. coli lac operon" protein_bind 2950..2971 /bound_moiety="E. coli catabolite activator protein" /vntifkey="31" /label=CAP\binding\site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3259..3847) /direction=LEFT /vntifkey="33" /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4018..4878) /codon_start=1 /gene="bla" /product="beta-lactamase" /vntifkey="4" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(4879..4983) /gene="bla" /vntifkey="30" /label=AmpR\promoter BASE COUNT 1167 a 1298 c 1277 g 1272 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2101 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 2161 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 2221 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 2281 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 2341 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 2401 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 2461 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 2521 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 2581 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 2641 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 2701 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 2761 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 2821 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 2881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 2941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 3481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 3721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 4021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 4201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4861 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4921 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4981 gcgcacattt ccccgaaaag tgccacctga cgtc //