LOCUS pEThisSMT_Human\ 6704 bp DNA circular 2-DEC-2024 SOURCE ORGANISM COMMENT http://www.informaxinc.com http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-15735656| COMMENT VNTDBDATE|-15735656| COMMENT LSOWNER| COMMENT VNTNAME|pEThisSMT_Human Wnt3a| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pEThisSMT_Human Wnt3a" 0 6704 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 6704 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 5 715 0 1961 0 2461 0 4396 COMMENT 0 6512 0 0 "") COMMENT #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 5903 0 6561 0 0 "") COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 5101 0 5105 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 5461 0 5707 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5243 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1145 0 0 "") COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) COMMENT (CObList COMMENT #10=(CFSignal (CObList) "Ap" 4 0 0 599 1456 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "599..1456") COMMENT #11=(CFSignal (CObList) "lacI" 4 0 1 3651 4607 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3651..4607)") COMMENT #12=(CFSignal (CObList) "T7P" 21 0 0 5125 5141 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5125..5141") COMMENT #13=(CFSignal (CObList) "T7T" 21 0 0 6633 6679 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6000..6046") COMMENT #14=(CFSignal (CObList) "SMT3" 4 0 0 5249 5541 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5249..5541") COMMENT #15=(CFSignal (CObList) "6*His" 94 0 0 5228 5245 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5228..5245") COMMENT #16=(CFSignal (CObList) "Bgl-SDM" 21 0 0 5350 5350 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5350..5350") COMMENT #17=(CFSignal (CObList) "bgl-blunt" 61 0 0 5098 5105 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5098..5105") COMMENT #18=(CFSignal (CObList) "T7\300\270\267\316 sq \310\256\300\316" 21 0 0 COMMENT 5177 373 0 (CStringList) (CStringList) 1 1 1 1 "5177..373") COMMENT #19=(CFSignal (CObList) "Human Wnt3a" 21 0 0 5543 6547 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #21=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "pEThisSMT_Human Wnt3a" 2) 5 "" 0 4) COMMENT #22=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pEThisSMT_Human Wnt3a'" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Local object" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 12/02/24 11:11\277\300\300\374" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 12/02/24 11:11\277\300\300\374" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6704 bp" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #30=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 14-APR-2022" 1))) COMMENT "Standard Fields") COMMENT #33=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #37=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 599 (3 #10# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 599 End: 1456" 1) COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 599..1456" 1))) "Ap") COMMENT #41=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3651 (3 #11# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3651 End: 4607 (Complementary)" COMMENT 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3651..4607)" 1))) "lacI") COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5249 (3 #14# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5249 End: 5541" 1) COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5249..5541" 1))) "SMT3"))) COMMENT "CDS (3 total)") COMMENT #49=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #50=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5125 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5125 End: 5141" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5125..5141" 1))) "T7P") COMMENT #54=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5177 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5177 End: 373 " 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5177..373" 1))) COMMENT "T7\300\270\267\316 sq \310\256\300\316") COMMENT #58=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5350 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5350 End: 5350" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5350..5350" 1))) "Bgl-SDM") COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5543 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5543 End: 6547" 1))) COMMENT "Human Wnt3a") COMMENT #64=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6633 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6633 End: 6679" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 6000..6046" 1))) "T7T"))) COMMENT "Misc. Feature (5 total)") COMMENT #68=(CFolderPar COMMENT (CGroupPar (CParagraph 61 (7 61 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #69=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5098 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5098 End: 5105" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5098..5105" 1))) "bgl-blunt"))) COMMENT "Exon (1 total)") COMMENT #73=(CFolderPar COMMENT (CGroupPar (CParagraph 94 (7 94 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #74=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5228 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5228 End: 5245" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5228..5245" 1))) "6*His"))) COMMENT "Signal Peptide (1 total)"))) "Feature Map") COMMENT #78=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #79=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103721432 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #80=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #81=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 715 " 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 1961 " 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191) COMMENT " N3: 2461 " 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (1 #0# 4) 1 2 2 0 191) COMMENT " N4: 4396 " 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (1 #0# 5) 1 2 2 0 191) COMMENT " N5: 6512 " 1))))) "ApaLI: 5 sites") 1 COMMENT 5 "GTGCAC" "CACGTG") COMMENT #86=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103721656 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #87=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #88=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 5903 " 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT " N2: 6561 " 1))))) "AvaI: 2 sites") 1 5 COMMENT "CYCGRG" "GRGCYC") COMMENT #90=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103721784 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #91=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103723160 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #92=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 5101 " 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 5105 " 1))))) "ClaI: 2 sites") 2 4 COMMENT "ATCGAT" "TAGCTA") COMMENT #95=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103722584 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #96=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 5461 " 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (1 #4# 2) 1 2 2 0 191) COMMENT " N2: 5707 " 1))))) "EcoRI: 2 sites") 1 COMMENT 5 "GAATTC" "CTTAAG") COMMENT #99=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103722616 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" COMMENT "TTCGAA") COMMENT #100=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103723288 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #101=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #102=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 5243 " 1))))) "NcoI: 1 site") 1 COMMENT 5 "CCATGG" "GGTACC") COMMENT #103=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103722648 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #104=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #105=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 1145 " 1))))) "PstI: 1 site") 5 COMMENT 1 "CTGCAG" "GACGTC") COMMENT #106=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103722392 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" COMMENT "GGGCCC") COMMENT #107=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 103722552 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" COMMENT "GGGCCC"))) "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #108=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #109=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #110=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #111=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #112=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #113=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #114=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #115=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #116=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #117=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #118=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #119=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #120=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #121=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #122=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #123=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #124=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #125=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #126=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #127=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #128=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #129=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #130=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #131=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #132=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #133=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #134=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #135=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #136=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #137=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #138=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #139=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #140=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #141=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #142=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #143=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #144=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #145=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #146=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #147=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) COMMENT 1 1 0 1 1 COMMENT (mapper: 18.4931 -24.9619 40.0378 40.0378 0.01 10 -1 6704 6704 1 0 0) COMMENT #148=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1300 100) COMMENT (CObjectList COMMENT #149=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 1684625264 100) COMMENT (CObjectList COMMENT #150=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 104036628 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27512 6.27312 1 0.0214037) 0.0935738) COMMENT #151=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #111# 312855558 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pEThisSMT Human Wnt3a" "@N" 1 0 COMMENT 0.871165 0 -10 13.9868 1.3737 Nil) COMMENT #152=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #110# 86 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "6704 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.42177 0.974079 Nil)) (CObjectList)) COMMENT #153=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1367 100) COMMENT (CObjectList COMMENT #154=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -669 100) COMMENT (CObjectList COMMENT #155=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #112# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 4.91075 5.71361 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #156=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 1633903476 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Ap" "@N" 1 COMMENT 10.8835 6.96932 0 -10 1.19887 1.09896 #155#) COMMENT #157=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #112# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 1.96224 2.85774 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #158=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 1734435683 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "lacI" "@N" 1 COMMENT -9.52936 -8.71165 0 -10 1.47361 1.09896 #157#) COMMENT #159=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #112# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 1.08826 1.36243 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #160=(CLabel (CWidget 0 (0 0) 1 2 0 0 #109# 37 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "SMT3" "@N" 1 COMMENT -13.1116 8.71165 0 -10 2.29783 1.09896 #159#)) COMMENT (CObjectList)) COMMENT #161=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil 539587699 100) COMMENT (CObjectList COMMENT #162=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #117# 46990144 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 1.47847 1.9 COMMENT 0.082322 1) COMMENT #163=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 12034128 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T7P" "@N" 1 COMMENT -12.8907 1.74233 0 -10 1.62347 1.09896 #162#) COMMENT #164=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #117# 46961664 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 0.0673731 COMMENT 1.9 0.082322 1) COMMENT #165=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 1633903476 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T7T" "@N" 1 COMMENT -2.91355 22.6503 0 -10 1.54854 1.09896 #164#) COMMENT #166=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #117# 103417264 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 1.26792 1.9 COMMENT 0.082322 1) COMMENT #167=(CLabel (CWidget 0 (0 0) 1 2 0 0 #109# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Bgl-SDM" "@N" 1 COMMENT -13.7965 6.96932 0 -10 3.54665 1.09896 #166#) COMMENT #168=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #113# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 5.92416 COMMENT 1.42981 1 0.082322) 0.8 1.8 0) COMMENT #169=(CLabel (CWidget 0 (0 0) 1 2 0 0 #109# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T7\300\270\267\316 sq \310\256\300\316" "@N" COMMENT 1 -10.3624 17.4233 0 -10 5.49481 1.09896 COMMENT #168#) COMMENT #170=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #113# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 0.146911 COMMENT 1.08733 1 0.082322) 0.8 1.8 0) COMMENT #171=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 1667458915 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Human Wnt3a" COMMENT "@N" 1 -11.1287 15.681 0 -10 5.59471 1.09896 COMMENT #170#)) (CObjectList)) COMMENT #172=(CGroupWidget COMMENT (CWidget 61 (7 61 0) 1 2 0 0 Nil -530 100) COMMENT (CObjectList COMMENT #173=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #117# 47551560 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 1.50373 1.9 COMMENT 0.082322 1) COMMENT #174=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #109# 1734566753 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "bgl-blunt" "@N" COMMENT 1 -14.1109 -3.48466 0 -10 3.47172 1.09896 COMMENT #173#)) (CObjectList)) COMMENT #175=(CGroupWidget COMMENT (CWidget 94 (7 94 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList COMMENT #176=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #130# 47597024 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 1.38208 1.9 COMMENT 0.082322 1) COMMENT #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #109# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "6*His" "@N" 1 COMMENT -13.0889 3.48466 0 -10 2.2229 1.09896 #176#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #178=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1018 100) COMMENT (CObjectList COMMENT #179=(CGroupWidget COMMENT (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -1157 100) COMMENT (CObjectList COMMENT #180=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #116# 47394872 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.36805 1.9 COMMENT 0.082322 1) COMMENT #181=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #118# 103518936 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Nco}I (5243)" "@N (@S)" 5 -14.1949 COMMENT 5.22699 0 -10 4.99528 0.974079 #180#)) COMMENT (CObjectList)) COMMENT #182=(CGroupWidget COMMENT (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -1297 100) COMMENT (CObjectList COMMENT #183=(CScratch COMMENT (CWidget 1 (1 #7# 1) 1 2 0 0 #116# 46960384 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 5.2027 1.9 COMMENT 0.082322 1) COMMENT #184=(CLabel (CWidget 0 (0 0) 1 2 0 0 #118# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (1145)" "@N (@S)" 5 12.9431 COMMENT 5.22699 0 -10 4.49575 0.974079 #183#)) COMMENT (CObjectList)) COMMENT #185=(CGroupWidget COMMENT (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -599 100) COMMENT (CObjectList COMMENT #186=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #116# 103418416 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.750462 1.9 COMMENT 0.082322 1) COMMENT #187=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# 6776679 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (5903)" "@N (@S)" 5 -11.3138 COMMENT 13.9386 0 -10 4.99528 0.974079 #186#) COMMENT #188=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #116# 47551496 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.134746 1.9 COMMENT 0.082322 1) COMMENT #189=(CLabel (CWidget 0 (0 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (6561)" "@N (@S)" 5 -4.95591 COMMENT 20.908 0 -10 4.92035 0.974079 #188#)) COMMENT (CObjectList)) COMMENT #190=(CGroupWidget COMMENT (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -1369 100) COMMENT (CObjectList COMMENT #191=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #116# 103419808 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.50092 1.9 COMMENT 0.082322 1) COMMENT #192=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# -2073674702 COMMENT 100) (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Cla}I (5101)" "@N (@S)" 5 -14.0102 COMMENT -1.74233 0 -10 4.49575 0.974079 #191#) COMMENT #193=(CScratch COMMENT (CWidget 2 (1 #3# 2) 1 2 0 0 #116# 103418560 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.49718 1.9 COMMENT 0.082322 1) COMMENT #194=(CLabel (CWidget 0 (0 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Cla}I (5105)" "@N (@S)" 5 -14.0665 0 0 COMMENT -10 4.57068 0.974079 #193#)) (CObjectList)) COMMENT #195=(CGroupWidget COMMENT (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -1088 100) COMMENT (CObjectList COMMENT #196=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #116# 47787992 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.16406 1.9 COMMENT 0.082322 1) COMMENT #197=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# 611 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Eco}RI (5461)" "@N (@S)" 5 -14.0779 COMMENT 10.454 0 -10 5.31997 0.974079 #196#) COMMENT #198=(CScratch COMMENT (CWidget 2 (1 #4# 2) 1 2 0 0 #116# 47596016 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.933866 1.9 COMMENT 0.082322 1) COMMENT #199=(CLabel (CWidget 0 (0 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Eco}RI (5707)" "@N (@S)" 5 -12.8676 COMMENT 12.1963 0 -10 5.3949 0.974079 #198#)) COMMENT (CObjectList)) COMMENT #200=(CGroupWidget COMMENT (CWidget 5 (10 #0# 0) 1 2 0 0 Nil 539587699 100) COMMENT (CObjectList COMMENT #201=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #116# 46990824 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 5.60507 1.9 COMMENT 0.082322 1) COMMENT #202=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# 1953785703 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (715)" "@N (@S)" 5 10.9145 COMMENT 8.71165 0 -10 4.84542 0.974079 #201#) COMMENT #203=(CScratch COMMENT (CWidget 2 (1 #0# 2) 1 2 0 0 #116# 22063536 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.43914 1.9 COMMENT 0.082322 1) COMMENT #204=(CLabel (CWidget 0 (0 0) 1 2 0 0 #108# 17 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (1961)" "@N (@S)" 5 14.042 COMMENT -3.48466 0 -10 5.34495 0.974079 #203#) COMMENT #205=(CScratch COMMENT (CWidget 3 (1 #0# 3) 1 2 0 0 #116# 103416832 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 3.97127 1.9 COMMENT 0.082322 1) COMMENT #206=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# 2082602632 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (2461)" "@N (@S)" 5 11.8193 COMMENT -8.71165 0 -10 5.3949 0.974079 #205#) COMMENT #207=(CScratch COMMENT (CWidget 4 (1 #0# 4) 1 2 0 0 #116# 47596632 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.16062 1.9 COMMENT 0.082322 1) COMMENT #208=(CLabel (CWidget 0 (0 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (4396)" "@N (@S)" 5 -12.8321 COMMENT -6.96932 0 -10 5.46983 0.974079 #207#) COMMENT #209=(CScratch COMMENT (CWidget 5 (1 #0# 5) 1 2 0 0 #116# 103417072 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.180597 1.9 COMMENT 0.082322 1) COMMENT #210=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #108# 2082602632 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (6512)" "@N (@S)" 5 -5.72379 COMMENT 19.1656 0 -10 5.3949 0.974079 #209#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #211=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 538976288 100) COMMENT (CObjectList) (CObjectList)) COMMENT #212=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 541412933 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #213=(AnlzFontColorItem 5542 6546 37173247)) 1 COMMENT (CObList #214=(AnlzFontBackColorItem 5248 5540 40304639))) (CObList) COMMENT 46458424 (CStringList) 93007240 1847395532 (CObList))) FEATURES Location/Qualifiers CDS 599..1456 /vntifkey="4" /label=Ap CDS complement(3651..4607) /vntifkey="4" /label=lacI misc_feature 5125..5141 /vntifkey="21" /label=T7P misc_feature 6633..6679 /vntifkey="21" /label=T7T CDS 5249..5541 /vntifkey="4" /label=SMT3 sig_peptide 5228..5245 /vntifkey="94" /label=6*His misc_feature 5350..5350 /vntifkey="21" /label=Bgl-SDM exon 5098..5105 /vntifkey="61" /label=bgl-blunt misc_feature 5177..373 /vntifkey="21" /label=T7À¸·Î\sq\È®ÀÎ misc_feature 5543..6547 /vntifkey="21" /label=Human\Wnt3a BASE COUNT 1582 a 1726 c 1792 g 1604 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata 5161 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgagagg 5221 atcgcatcac catcaccatc accatggaat gtcggactca gaagtcaatc aagaagctaa 5281 gccagaggtc aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg 5341 atcttcagaa atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc 5401 gttcgctaaa agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag 5461 aattcaagct gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc 5521 tcacagagaa cagattggtg gcagttaccc gatctggtgg tccttggctg ttggtccgca 5581 atattctagc ttaggttccc agcccattct gtgtgcgtca attccaggct tagtgccgaa 5641 gcaacttcgt ttctgccgta attatgttga gattatgccc agcgtcgcag aaggaatcaa 5701 gattggaatt caagaatgtc agcatcagtt ccgtggtcgc cgctggaatt gtaccaccgt 5761 ccatgattca ctggctattt ttggtccagt gcttgacaaa gcgacgcgcg aatcggcttt 5821 cgttcacgct atcgcctctg ccggagtggc gttcgcggtc acgcgtagct gcgccgaggg 5881 cacggcggca atttgtgggt gctcgagccg tcatcaaggt tcacctggta aggggtggaa 5941 atggggaggt tgttcggagg atatcgaatt tgggggcatg gtctcgcgcg aatttgctga 6001 cgcacgcgaa aaccgcccgg acgcgcgtag tgccatgaat cgtcataata atgaagcggg 6061 gcgtcaggcg atcgcgtccc atatgcatct gaaatgcaag tgccacggat taagcggaag 6121 ctgcgaggta aagacttgct ggtggtcaca acctgacttt cgcgctattg gcgactttct 6181 gaaagataag tacgattcag catcagaaat ggttgtcgaa aagcaccgtg agtcacgcgg 6241 atgggttgaa acgttgcgcc cacgttatac ttatttcaaa gttcccaccg aacgtgactt 6301 agtatattac gaggcctctc caaacttttg tgaacccaac cctgagacag ggtcatttgg 6361 aacacgtgat cgcacgtgta acgtgagctc acacggaatc gacgggtgcg acttactgtg 6421 ttgtggccgc ggtcataatg cccgtgcaga gcgccgtcgt gagaagtgtc gctgcgtctt 6481 ccattggtgc tgttatgtca gctgtcagga gtgcacgcgc gtttacgacg tccacacgtg 6541 taaatgagtc taaactctcc tcgagtgaga tccggctgct aacaaagccc gaaaggaagc 6601 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg 6661 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat //