LOCUS RC2205-06\pET-28 7058 bp DNA linear 13-JUN-2022 DEFINITION Complementary copy of 210617 pET-28. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-14387996| COMMENT VNTDBDATE|-14435526| COMMENT LSOWNER| COMMENT VNTNAME|RC2205-06 pET-28 rc+smt3-poly(A) polymerase(SMT)| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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End: 6852" 1) COMMENT #123=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5131..6857" 1))) "T7_leader") COMMENT #125=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5425 (3 #29# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #126=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5425 End: 6822" 1) COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5430..6827" 1))) COMMENT "poly(A) polymerase") COMMENT #129=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6972 (3 #27# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #130=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6972 End: 6990 (Complementary)" COMMENT 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(6977..6995)" 1))) COMMENT "T7_Terminal_primer"))) COMMENT "Misc. Feature (10 total)") COMMENT #133=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #134=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4984 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #135=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4984 End: 5002" 1) COMMENT #136=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #137=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4984..5002" 1))) "T7_promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #138=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #139=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1481 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #140=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1481 End: 2100" 1) COMMENT #141=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #142=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1481..2100" 1))) "pBR322_origin"))) COMMENT "Replication Origin (1 total)") COMMENT #143=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #144=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6930 (3 #28# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #145=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6930 End: 7058" 1) COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #147=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 6935..7063" 1))) "T7_terminator"))) COMMENT "Terminator (1 total)"))) "Feature Map") COMMENT #148=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #149=(CFolderPar COMMENT (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #150=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 560 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #151=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 560 End: 1375 (Complementary)" COMMENT 1) COMMENT #152=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #153=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(560..1375)" 1) COMMENT #154=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #155=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"KanR2\"" 1))) "KanR2") COMMENT #156=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3181 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #157=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3181 End: 3456 (Complementary)" COMMENT 1) COMMENT #158=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #159=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3181..3456)" 1) COMMENT #160=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #161=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (576 - 851)\"" 1))) COMMENT "tet (576 - 851)") COMMENT #162=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4689 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #163=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4689 End: 4952 (Complementary)" COMMENT 1) COMMENT #164=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #165=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4689..4952)" 1) COMMENT #166=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #167=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"tet (300 - 563)\"" 1))) COMMENT "tet (300 - 563)"))) "Gene (3 total)") COMMENT #168=(CFolderPar COMMENT (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #169=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #170=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 7058 (Complementary)" COMMENT 1) COMMENT #171=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #172=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1..7063)" 1) COMMENT #173=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #174=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /organism=\"pET-28 a (+)\"" 1) COMMENT #175=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /mol_type=\"other DNA\"" 1))) COMMENT "Source_1"))) "Source (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #176=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #177=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 20843488 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #178=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #179=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 6397 " 1) COMMENT #180=(CLinePar COMMENT (CParagraph 0 (1 #3# 2) 1 2 2 0 191) COMMENT " N2: 6816 " 1))))) "KpnI: 2 sites") COMMENT 5 1 "GGTACC" "CCATGG") COMMENT #181=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 20843712 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #182=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #183=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 5070 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #184=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 20843456 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #185=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #186=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 5130 " 1))))) "NdeI: 1 site") COMMENT 2 4 "CATATG" "GTATAC") COMMENT #187=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 20842976 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #188=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #189=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 6897 " 1))))) "XhoI: 1 site") COMMENT 1 5 "CTCGAG" "GAGCTC"))) COMMENT "Restriction/Methylation Map") COMMENT #190=(CFolderPar COMMENT (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #191=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #192=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #193=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372064 (4 #40# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #194=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #195=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3256 - 3729" COMMENT 1))) "3256(d1) (158 codons)") COMMENT #196=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373072 (4 #43# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #197=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #198=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4438 - 4608" COMMENT 1))) "4438(d1) (57 codons)") COMMENT #199=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371728 (4 #44# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #200=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #201=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4636 - 4896" COMMENT 1))) "4636(d1) (87 codons)") COMMENT #202=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372232 (4 #50# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #203=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #204=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5071 - 6819" COMMENT 1))) "5071(d1) (583 codons)"))) COMMENT "Phase #1 (4 frames)") COMMENT #205=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #206=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94374192 (4 #30# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #207=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tag" COMMENT 1) COMMENT #208=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 47 - 226" 1))) COMMENT "47(d2) (60 codons)") COMMENT #209=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371616 (4 #32# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #210=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #211=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2738 - 3103" COMMENT 1))) "2738(d2) (122 codons)") COMMENT #212=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371952 (4 #35# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #213=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #214=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3329 - 3478" COMMENT 1))) "3329(d2) (50 codons)") COMMENT #215=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373016 (4 #52# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #216=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #217=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4262 - 4579" COMMENT 1))) "4262(d2) (106 codons)"))) COMMENT "Phase #2 (4 frames)") COMMENT #218=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #219=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373128 (4 #45# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #220=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tag" COMMENT 1) COMMENT #221=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1080 - 1253" COMMENT 1))) "1080(d3) (58 codons)") COMMENT #222=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372120 (4 #49# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #223=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #224=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4773 - 5009" COMMENT 1))) "4773(d3) (79 codons)"))) COMMENT "Phase #3 (2 frames)"))) "Direct strand") COMMENT #225=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #226=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (14 -1 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #227=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94374640 (4 #36# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #228=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #229=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 1581 - 1832" COMMENT 1))) "1581(c1) (84 codons)") COMMENT #230=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372848 (4 #37# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #231=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #232=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2142 - 2321" COMMENT 1))) "2142(c1) (60 codons)") COMMENT #233=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372792 (4 #38# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #234=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #235=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2406 - 2612" COMMENT 1))) "2406(c1) (69 codons)") COMMENT #236=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372400 (4 #39# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #237=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #238=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2901 - 3098" COMMENT 1))) "2901(c1) (66 codons)") COMMENT #239=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371000 (4 #41# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #240=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #241=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3498 - 3758" COMMENT 1))) "3498(c1) (87 codons)") COMMENT #242=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372288 (4 #42# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #243=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #244=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4350 - 4940" COMMENT 1))) "4350(c1) (197 codons)") COMMENT #245=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373520 (4 #58# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #246=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #247=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5406 - 5687" COMMENT 1))) "5406(c1) (94 codons)") COMMENT #248=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373352 (4 #59# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #249=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #250=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 5754 - 5915" COMMENT 1))) "5754(c1) (54 codons)") COMMENT #251=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371056 (4 #60# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #252=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #253=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6627 - 6896" COMMENT 1))) "6627(c1) (90 codons)"))) COMMENT "Phase #1 (9 frames)") COMMENT #254=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2 (14 -2 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #255=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372904 (4 #31# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #256=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: taa" COMMENT 1) COMMENT #257=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 563 - 1375" COMMENT 1))) "563(c2) (271 codons)") COMMENT #258=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372456 (4 #33# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #259=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: taa" COMMENT 1) COMMENT #260=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2813 - 3073" COMMENT 1))) "2813(c2) (87 codons)") COMMENT #261=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94374696 (4 #34# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #262=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #263=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3098 - 3421" COMMENT 1))) "3098(c2) (108 codons)") COMMENT #264=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372680 (4 #51# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #265=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #266=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3518 - 4606" COMMENT 1))) "3518(c2) (363 codons)") COMMENT #267=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94373688 (4 #53# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #268=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #269=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 4847 - 4996" COMMENT 1))) "4847(c2) (50 codons)") COMMENT #270=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94372624 (4 #54# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #271=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tga" COMMENT 1) COMMENT #272=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 6308 - 6589" COMMENT 1))) "6308(c2) (94 codons)"))) COMMENT "Phase #2 (6 frames)") COMMENT #273=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3 (14 -3 0) 1 1 2 0 178) COMMENT (CObjectList COMMENT #274=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94374472 (4 #46# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #275=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: gtg Stop codon: tga" COMMENT 1) COMMENT #276=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 2518 - 2874" COMMENT 1))) "2518(c3) (119 codons)") COMMENT #277=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 94371224 (4 #47# 0) 1 2 COMMENT 3 0 192) COMMENT (CObjectList COMMENT #278=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) COMMENT "Start codon: atg Stop codon: tag" COMMENT 1) COMMENT #279=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 4 0 COMMENT 180) "Region: 3106 - 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#452=(AnlzFontBackColorItem 5424 5424 40304639))) (CObList) 46028640 COMMENT (CStringList) 94689512 1386832007 (CObList))) FEATURES Location/Qualifiers CDS complement(560..1375) /vntifkey="4" /label=ORF\frame\2 gene complement(560..1375) /gene="KanR2" /vntifkey="60" rep_origin 1481..2100 /vntifkey="33" /label=pBR322_origin misc_feature 2499..2521 /vntifkey="21" /label=pGEX_3_primer misc_feature complement(2515..2706) /vntifkey="21" /label=ROP gene complement(3181..3456) /gene="tet (576 - 851)" /vntifkey="60" CDS complement(3515..4474) /vntifkey="4" /label=ORF\frame\2 misc_feature complement(3515..4606) /vntifkey="21" /label=lacI CDS complement(4347..4847) /vntifkey="4" /label=ORF\frame\1 misc_feature 4881..4900 /vntifkey="21" /label=pBRrevBam_primer gene complement(4689..4952) /gene="tet (300 - 563)" /vntifkey="60" promoter 4984..5002 /vntifkey="30" /label=T7_promoter misc_feature 5002..5029 /vntifkey="21" /label=lacO misc_feature 5047..5063 /vntifkey="21" /label=T7_transl_en_RBS misc_feature 5130..6837 /vntifkey="21" /label=Xpress_fwd_primer misc_feature 5131..6852 /vntifkey="21" /label=T7_leader misc_feature complement(6972..6990) /vntifkey="21" /label=T7_Terminal_primer terminator 6930..7058 /vntifkey="43" /label=T7_terminator source complement(1..7058) /organism="pET-28 a (+)" /mol_type="other DNA" /vntifkey="98" misc_feature 5425..6822 /vntifkey="21" /label=poly(A)\polymerase BASE COUNT 1688 a 1890 c 1853 g 1627 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac 5101 agcagcggcc tggtgccgcg cggcagccat atgtcggact cagaagtcaa tcaagaagct 5161 aagccagagg tcaagccaga agtcaagcct gagactcaca tcaatttaaa ggtgtccgat 5221 ggatcttcag aaatcttctt caagatcaaa aagaccactc ctttaagaag gctgatggaa 5281 gcgttcgcta aaagacaggg taaggaaatg gactccttaa gattcttgta cgacggtatt 5341 agaattcaag ctgatcagac ccctgaagat ttggacatgg aggataacga tattattgag 5401 gctcacagag aacagattgg tgggatgttt acccgagtcg ctaatttttg ccgcaaggtg 5461 ctaagccgcg aggaaagcga ggctgaacag gcagtcgccc gtccacaggt gacggtgatc 5521 ccgcgtgagc agcatgctat ttcccgcaaa gatatcagtg aaaatgccct gaaggtaatg 5581 tacaggctca ataaagcggg atacgaagcc tggctggttg gcggcggcgt gcgcgacctg 5641 ttacttggca aaaagccgaa agattttgac gtaaccacta acgccacgcc tgagcaggtg 5701 cgcaaactgt tccgtaactg ccgcctggtg ggtcgccgtt tccgtctggc tcatgtaatg 5761 tttggcccgg agattatcga agttgcgacc ttccgtggac accacgaagg taacgtcagc 5821 gaccgcacga cctcccaacg cgggcaaaac ggcatgttgc tgcgcgacaa cattttcggc 5881 tccatcgaag aagacgccca gcgccgcgat ttcactatca acagcctgta ttacagcgta 5941 gcggatttta ccgtccgtga ttacgttggc ggcatgaagg atctgaagga cggcgttatc 6001 cgtctgattg gtaacccgga aacgcgctac cgtgaagatc cggtacgtat gctgcgcgcg 6061 gtacgttttg ccgccaaatt gggtatgcgc atcagcccgg aaaccgcaga accgatccct 6121 cgcctcgcta ccctgctgaa cgatatccca ccggcacgcc tgtttgaaga atcgcttaaa 6181 ctgctacaag cgggctacgg ttacgaaacc tataagctgt tgtgtgaata tcatctgttc 6241 cagccgctgt tcccgaccat tacccgctac ttcacggaaa atggcgacag cccgatggag 6301 cggatcattg aacaggtgct gaagaatacc gatacgcgta tccataacga tatgcgcgtg 6361 aacccggcgt tcctgtttgc cgccatgttc tggtacccac tgctggagac ggcacagaag 6421 atcgcccagg aaagcggcct gacctatcac gacgctttcg cgctggcgat gaacgacgtg 6481 ctggacgaag cctgccgttc actggcaatc ccgaaacgtc tgacgacatt aacccgcgat 6541 atctggcagt tgcagttgcg tatgtcccgt cgtcagggta aacgcgcatg gaaactgctg 6601 gagcatccta agttccgtgc ggcttatgac ctgttggcct tgcgagctga agttgagcgt 6661 aacgctgaac tgcagcgtct ggtgaaatgg tggggtgagt tccaggtttc cgcgccacca 6721 gaccaaaaag ggatgctcaa cgagctggat gaagaaccgt caccgcgtcg tcgtactcgt 6781 cgtccacgca aacgcgcacc acgtcgtgag ggtaccgcat gagctagcat gactggtgga 6841 cagcaaatgg gtcgcggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga 6901 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt 6961 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 7021 gaggggtttt ttgctgaaag gaggaactat atccggat //