LOCUS CS17-109-04-pPIC 9835 bp DNA circular 27-NOV-2017 DEFINITION pPIC9K. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12046373| COMMENT VNTDBDATE|-12046373| COMMENT LSOWNER| COMMENT VNTNAME|CS17-109-04-pPIC9K-DNase I(-ес-MF)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| FEATURES Location/Qualifiers source 1..9835 /organism="pPIC9K" /mol_type="other DNA" /vntifkey="98" misc_feature complement(1886..1906) /vntifkey="21" /label=3_AOX1_primer CDS complement(2539..5073) /vntifkey="4" /label=ORF\frame\2 gene complement(5487..6302) /gene="KanR2" /vntifkey="60" CDS complement(5487..6302) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(6467..6486) /vntifkey="21" /label=pBRrevBam_primer CDS complement(6468..7190) /vntifkey="4" /label=ORF\frame\3 misc_feature complement(7472..7494) /vntifkey="21" /label=pGEX_3_primer rep_origin complement(7891..8510) /vntifkey="33" /label=pBR322_origin gene complement(8665..9525) /gene="Ampicillin" /vntifkey="60" CDS complement(8665..9525) /vntifkey="4" /label=ORF\frame\2 promoter complement(9567..9595) /vntifkey="30" /label=AmpR_promoter misc_feature 944..1792 /vntifkey="21" /label=DNase\I(-ес-MF) BASE COUNT 2578 a 2395 c 2249 g 2613 t ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccatgaggg gcaccaggct 961 gatggggctg ctgctcgccc tcgctggtct gctgcagctg ggcttgtccc tgaagatagc 1021 agccttcaac atccgcacct ttggggagac caagatgtcc aatgctacgc tcgccagcta 1081 cattgttcgg atcgtgcgtc gttacgacat cgtcctcatc caggaggtca gagacagcca 1141 cctggtggct gtggggaagc tcctggacta tctcaaccag gatgacccaa acacctacca 1201 ctatgtggtc agtgagccgc tgggccgcaa cagctacaag gagcgctacc tctttctgtt 1261 cagacccaac aaggtgtccg tgctggacac ctaccagtac gacgacggct gcgagtcctg 1321 cgggaacgac agcttcagcc gggagcccgc tgtggtcaag ttctcatccc actccaccaa 1381 ggtcaaggaa tttgccattg ttgccctgca ctcggcccca tcggacgcag tggctgagat 1441 taattctctc tacgatgtct acctggatgt ccagcagaag tggcacttga acgatgtcat 1501 gttgatgggc gatttcaatg ctgactgcag ctacgtgacc tcctcgcagt ggtcatccat 1561 ccgcctgcgt acgagctcca ccttccagtg gctgattcct gacagtgccg acaccacggc 1621 tacgtccacg aactgcgcct atgacaggat cgtggtcgca gggtctctgc tccagagttc 1681 tgtggttcct ggctcggccg ctccctttga cttccaagct gcatacggac tgagcaatga 1741 gatggccctg gccatcagtg accattaccc ggtggaggtg acgctgacat aagcggccgc 1801 gaattaattc gccttagaca tgactgttcc tcagttcaag ttgggcactt acgagaagac 1861 cggtcttgct agattctaat caagaggatg tcagaatgcc atttgcctga gagatgcagg 1921 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt 1981 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagctgat gaatatcttg 2041 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc 2101 ttcagagtac agaagattaa gtgagaagtt cgtttgtgca agcttatcga taagctttaa 2161 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac 2221 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt 2281 atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac 2341 tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga 2401 gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact 2461 atcgactacg cgatcatggc gaccacaccc gtcctgtgga tctatcgaat ctaaatgtaa 2521 gttaaaatct ctaaataatt aaataagtcc cagtttctcc atacgaacct taacagcatt 2581 gcggtgagca tctagacctt caacagcagc cagatccatc actgcttggc caatatgttt 2641 cagtccctca ggagttacgt cttgtgaagt gatgaacttc tggaaggttg cagtgttaac 2701 tccgctgtat tgacgggcat atccgtacgt tggcaaagtg tggttggtac cggaggagta 2761 atctccacaa ctctctggag agtaggcacc aacaaacaca gatccagcgt gttgtacttg 2821 atcaacataa gaagaagcat tctcgatttg caggatcaag tgttcaggag cgtactgatt 2881 ggacatttcc aaagcctgct cgtaggttgc aaccgatagg gttgtagagt gtgcaataca 2941 cttgcgtaca atttcaaccc ttggcaactg cacagcttgg ttgtgaacag catcttcaat 3001 tctggcaagc tccttgtctg tcatatcgac agccaacaga atcacctggg aatcaatacc 3061 atgttcagct tgagacagaa ggtctgaggc aacgaaatct ggatcagcgt atttatcagc 3121 aataactaga acttcagaag gcccagcagg catgtcaata ctacacaggg ctgatgtgtc 3181 attttgaacc atcatcttgg cagcagtaac gaactggttt cctggaccaa atattttgtc 3241 acacttagga acagtttctg ttccgtaagc catagcagct actgcctggg cgcctcctgc 3301 tagcacgata cacttagcac caaccttgtg ggcaacgtag atgacttctg gggtaagggt 3361 accatccttc ttaggtggag atgcaaaaac aatttctttg caaccagcaa ctttggcagg 3421 aacacccagc atcagggaag tggaaggcag aattgcggtt ccaccaggaa tatagaggcc 3481 aactttctca ataggtcttg caaaacgaga gcagactaca ccagggcaag tctcaacttg 3541 caacgtctcc gttagttgag cttcatggaa tttcctgacg ttatctatag agagatcaat 3601 ggctctctta acgttatctg gcaattgcat aagttcctct gggaaaggag cttctaacac 3661 aggtgtcttc aaagcgactc catcaaactt ggcagttagt tctaaaaggg ctttgtcacc 3721 attttgacga acattgtcga caattggttt gactaattcc ataatctgtt ccgttttctg 3781 gataggacga cgaagggcat cttcaatttc ttgtgaggag gccttagaaa cgtcaatttt 3841 gcacaattca atacgacctt cagaagggac ttctttaggt ttggattctt ctttaggttg 3901 ttccttggtg tatcctggct tggcatctcc tttccttcta gtgaccttta gggacttcat 3961 atccaggttt ctctccacct cgtccaacgt cacaccgtac ttggcacatc taactaatgc 4021 aaaataaaat aagtcagcac attcccaggc tatatcttcc ttggatttag cttctgcaag 4081 ttcatcagct tcctccctaa ttttagcgtt caacaaaact tcgtcgtcaa ataaccgttt 4141 ggtataagaa ccttctggag cattgctctt acgatcccac aaggtggctt ccatggctct 4201 aagacccttt gattggccaa aacaggaagt gcgttccaag tgacagaaac caacacctgt 4261 ttgttcaacc acaaatttca agcagtctcc atcacaatcc aattcgatac ccagcaactt 4321 ttgagttgct ccagatgtag cacctttata ccacaaaccg tgacgacgag attggtagac 4381 tccagtttgt gtccttatag cctccggaat agactttttg gacgagtaca ccaggcccaa 4441 cgagtaatta gaagagtcag ccaccaaagt agtgaataga ccatcggggc ggtcagtagt 4501 caaagacgcc aacaaaattt cactgacagg gaactttttg acatcttcag aaagttcgta 4561 ttcagtagtc aattgccgag catcaataat ggggattata ccagaagcaa cagtggaagt 4621 cacatctacc aactttgcgg tctcagaaaa agcataaaca gttctactac cgccattagt 4681 gaaacttttc aaatcgccca gtggagaaga aaaaggcaca gcgatactag cattagcggg 4741 caaggatgca actttatcaa ccagggtcct atagataacc ctagcgcctg ggatcatcct 4801 ttggacaact ctttctgcca aatctaggtc caaaatcact tcattgatac cattattgta 4861 caacttgagc aagttgtcga tcagctcctc aaattggtcc tctgtaacgg atgactcaac 4921 ttgcacatta acttgaagct cagtcgattg agtgaacttg atcaggttgt gcagctggtc 4981 agcagcatag ggaaacacgg cttttcctac caaactcaag gaattatcaa actctgcaac 5041 acttgcgtat gcaggtagca agggaaatgt catacttgaa gtcggacagt gagtgtagtc 5101 ttgagaaatt ctgaagccgt atttttatta tcagtgagtc agtcatcagg agatcctcta 5161 cgccggacgc atcgtggccg acctgcaggg gggggggggg cgctgaggtc tgcctcgtga 5221 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag 5281 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 5341 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 5401 aaaagttcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag 5461 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc 5521 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 5581 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 5641 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 5701 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt 5761 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca 5821 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta 5881 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca 5941 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg 6001 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga 6061 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca 6121 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga 6181 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca 6241 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc 6301 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata 6361 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc 6421 cccctgcagg tcggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac 6481 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg 6541 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca 6601 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag 6661 gagtcgcata agggagagcg tcgagtatct atgattggaa gtatgggaat ggtgataccc 6721 gcattcttca gtgtcttgag gtctcctatc agattatgcc caactaaagc aaccggagga 6781 ggagatttca tggtaaattt ctctgacttt tggtcatcag tagactcgaa ctgtgagact 6841 atctcggtta tgacagcaga aatgtccttc ttggagacag taaatgaagt cccaccaata 6901 aagaaatcct tgttatcagg aacaaacttc ttgtttcgaa ctttttcggt gccttgaact 6961 ataaaatgta gagtggatat gtcgggtagg aatggagcgg gcaaatgctt accttctgga 7021 ccttcaagag gtatgtaggg tttgtagata ctgatgccaa cttcagtgac aacgttgcta 7081 tttcgttcaa accattccga atccagagaa atcaaagttg tttgtctact attgatccaa 7141 gccagtgcgg tcttgaaact gacaatagtg tgctcgtgtt ttgaggtcat ctttgtatga 7201 ataaatctag tctttgatct aaataatctt gacgagccaa ggcgataaat acccaaatct 7261 aaaactcttt taaaacgtta aaaggacaag tatgtctgcc tgtattaaac cccaaatcag 7321 ctcgtagtct gatcctcatc aacttgaggg gcactatctt gttttagaga aatttgcgga 7381 gatgcgatat cgagaaaaag gtacgctgat tttaaacgtg aaatttatct caagatctct 7441 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 7501 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 7561 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 7621 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 7681 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 7741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8101 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 9001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 9301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9661 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9721 tcgtcttcaa gaattaattc tcatgtttga cagcttatca tcgataagct gactcatgtt 9781 ggtattgtga aatagacgca gatcgggaac actgaaaaat aacagttatt attcg //