LOCUS CS18-129-02-pcDN 6833 bp DNA circular 6-SEP-2024 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-12562165| COMMENT VNTDBDATE|-15610741| COMMENT LSOWNER| COMMENT VNTNAME|CS18-129-02-pcDNA-MCS| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Location/Qualifiers misc_feature 5655..6512 /vntifkey="21" /label=AmpR misc_feature 430..633 /vntifkey="21" /label=CMV\promoter misc_feature 770..1558 /vntifkey="21" /label=hR-Spondin\1\(21-263)CHO\optimization BASE COUNT 1547 a 1814 c 1762 g 1710 t ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tggatccgaa ttcaagcttg cggccgcaca tgcggctcgg 781 cctctgcgtt gtagctttgg tccttagctg gacacatctt actatctcca gtagagggat 841 aaaagggaag agacaacggc ggatatctgc tgaaggttct caagcttgtg ccaaaggttg 901 cgagttgtgt tccgaagtga atggttgtct caagtgctcc cctaagttgt ttatcctgtt 961 ggagcgaaat gatattcgac aagtaggggt ctgccttcct tcctgtcctc caggttactt 1021 cgacgcccga aatcccgaca tgaataaatg tataaaatgt aagatcgagc attgtgaagc 1081 ctgtttttcc cataactttt gtaccaaatg taaagaaggt ttgtacctgc ataaaggtag 1141 atgctacccc gcatgtcctg agggctcctc tgctgcaaac ggaactatgg aatgctcaag 1201 ccctgctcaa tgcgaaatgt cagagtggtc accttggggc ccttgtagca agaagcagca 1261 gctctgtggt tttcggcggg ggagtgaaga aagaacacgg agggtacttc acgcccccgt 1321 tggggaccac gccgcttgtt cagatacaaa ggagacaaga aggtgcacag ttcgacgggt 1381 tccctgccct gaagggcaaa aacgacgcaa aggcggacag ggacgaaggg aaaatgccaa 1441 cagaaatctg gccagaaaag agagtaagga ggctggggcc gggtcccgcc gccgcaaagg 1501 tcaacagcag caacagcaac agggtacagt tggtccattg acatccgctg gccccgcctc 1561 gagaagggtt cgatccctac cggttagtaa tgagtttgat atctcgacaa tcaacctctg 1621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 1681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 1741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 1801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 1861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 1921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 1981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 2041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 2101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 2161 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg 2221 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 2281 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 2341 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 2401 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 2461 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc 2521 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2581 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 2641 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta 2701 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 2761 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 2821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 2881 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 2941 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 3001 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 3061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 3241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 3301 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 3361 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 3421 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 3481 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 3541 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 3601 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 3661 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 3721 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 3781 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 3841 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 3901 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 3961 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 4021 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 4081 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 4141 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca 4201 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4261 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 4441 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 4501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 4681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 5101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 5341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 6001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 6061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 6661 gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 6721 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 6781 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg //