LOCUS pET28a-Cas9-His- 9346 bp DNA circular 6-NOV-2014 DEFINITION Complementary copy of CS822-01-pet28a-Cas9. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|713878361| COMMENT VNTDBDATE|713878519| COMMENT LSOWNER| COMMENT VNTNAME|pET28a-Cas9-His-D24E| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#170=(XLATFRAGMENT 5070 1366 0 1 5071 4100) COMMENT #171=(XLATFRAGMENT 9171 12 0 1 9172 38)) COMMENT (CObList #172=(AnlzXLATItem 5070 9170 1 5070 9173) COMMENT #173=(AnlzXLATItem 9171 9209 1 9171 9209)) 1 (CObList)) (CObList) COMMENT 83842144 (CStringList) 84034424 1917439865 (CObList))) FEATURES Location/Qualifiers misc_feature 9190..9207 /vntifkey="21" /label=his-tag misc_feature 5071..9210 /vntifkey="21" /label=Cas9-D24E misc_feature 5142..5142 /vntifkey="21" /label=Muta. BASE COUNT 2695 a 2025 c 2175 g 2451 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atggataaga aatactcaat aggcttagat 5101 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa 5161 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct 5221 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga 5281 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg 5341 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac 5401 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag 5461 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat 5521 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt 5581 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa 5641 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg 5701 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc 5761 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct 5821 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact 5881 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt 5941 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact 6001 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa 6061 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc 6121 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa 6181 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg 6241 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt 6301 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat 6361 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat 6421 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa 6481 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca 6541 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa 6601 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt 6661 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat 6721 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa 6781 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca 6841 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa 6901 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag 6961 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag 7021 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt 7081 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat 7141 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa 7201 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc 7261 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta 7321 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact 7381 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa 7441 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag 7501 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt 7561 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat 7621 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt 7681 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag 7741 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa 7801 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat 7861 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt 7921 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc 7981 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat 8041 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat 8101 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc 8161 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt 8221 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga 8281 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc 8341 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt 8401 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa 8461 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa 8521 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa 8581 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt 8641 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt 8701 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc 8761 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa 8821 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt 8881 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt 8941 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt 9001 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca 9061 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa 9121 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgacgcggcc 9181 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa 9241 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa 9301 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat //