LOCUS pEThis-SMT-Cas9- 9811 bp DNA circular 6-NOV-2014 SOURCE ORGANISM COMMENT http://www.informaxinc.com http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|713878959| COMMENT VNTDBDATE|713878959| COMMENT LSOWNER| COMMENT VNTNAME|pEThis-SMT-Cas9-D24E| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 599..1456 /vntifkey="4" /label=Ap CDS complement(3651..4607) /vntifkey="4" /label=lacI misc_feature 5125..5141 /vntifkey="21" /label=T7P misc_feature 9740..9786 /vntifkey="21" /label=T7T CDS 5249..5541 /vntifkey="4" /label=SMT3 sig_peptide 5228..5245 /vntifkey="94" /label=6*His misc_feature 9664..9669 /vntifkey="21" /label=BseRI misc_feature 5350..5350 /vntifkey="21" /label=Bgl-SDM exon 5098..5105 /vntifkey="61" /label=bgl-blunt misc_feature 5543..9649 /vntifkey="21" /label=Cas9-D24E misc_feature 5614..5614 /vntifkey="21" /label=Muta BASE COUNT 2822 a 2101 c 2327 g 2561 t ORIGIN 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata 5161 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgagagg 5221 atcgcatcac catcaccatc accatggaat gtcggactca gaagtcaatc aagaagctaa 5281 gccagaggtc aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg 5341 atcttcagaa atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc 5401 gttcgctaaa agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag 5461 aattcaagct gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc 5521 tcacagagaa cagattggtg ggatggataa gaaatactca ataggcttag atatcggcac 5581 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa 5641 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt 5701 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac 5761 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt 5821 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca 5881 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc 5941 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt 6001 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga 6061 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa 6121 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc 6181 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa 6241 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa 6301 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga 6361 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc 6421 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac 6481 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac 6541 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga 6601 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta 6661 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact 6721 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca 6781 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt 6841 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg 6901 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat 6961 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga 7021 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt 7081 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg 7141 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt 7201 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga 7261 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac 7321 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga 7381 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga 7441 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg 7501 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa 7561 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt 7621 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt 7681 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat 7741 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg 7801 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg 7861 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag 7921 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct 7981 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt 8041 aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga 8101 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga 8161 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac 8221 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa 8281 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca 8341 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt 8401 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta 8461 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt 8521 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta 8581 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac 8641 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc 8701 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt 8761 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa 8821 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa 8881 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg 8941 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa 9001 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc 9061 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga 9121 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat 9181 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt 9241 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga 9301 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat 9361 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc 9421 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt 9481 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg 9541 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac 9601 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgag taccgtctaa 9661 actctcctcg agtgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct 9721 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt 9781 tttttgctga aaggaggaac tatatccgga t //