McCoy's 5A, 15% Non-heat inactivated FBS, 1% Abs,
0.4X MEM vitamins (100X), 1X MEM non-essential amino acids (100X), 0.5X essential amino acids (50X), 1 mM sodium pyruvate, 2 mM L-Glutamine (LM005-01, 1.5 mM L-Glu)  
Just before use,
0.05 mM β-ME, 50 ng/ml hIL-7